| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,070,246:1 | +GA | 100% | intergenic (+46/+96) | aroP‑I → / ← PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,070,246 | 1 | . | G | 93.8% | 144.6 / 3.3 | 48 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (21/23); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,070,246 | 2 | . | A | 93.8% | 138.2 / 2.9 | 48 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base A (21/24); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAAC > NC_002947/1070100‑1070384 || tGACGCCGCCGATTCGCATCTCGGTAATGCTGCTCCAGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑Ct‑‑gt > 8:82194/1‑147 (MQ=255) cATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCTTTAGGCTTTCATCGC‑‑CT‑‑GTGAGATCGAGCGCcgcccg > 7:105164/1‑149 (MQ=38) cATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCcgcgcg > 8:283698/1‑149 (MQ=255) cATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCcgcgcg > 7:161374/1‑149 (MQ=255) aTCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCgcgg < 5:45578/149‑1 (MQ=255) tcGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGACAAGTGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCgcgggcg > 7:285662/1‑149 (MQ=39) ggTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCgcggg < 6:111776/145‑1 (MQ=255) aaTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGGTGTCAAGCTGAAGAAGTCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTATGTTTTCGGCGC‑‑CTGCGA‑‑GATCGAGCGCCGCCCGCGCGGCGCt < 3:23640/149‑1 (MQ=38) aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCTTTAGGCTTTCATCGC‑‑CT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCt < 8:105164/148‑1 (MQ=37) aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCt < 6:36774/148‑1 (MQ=255) aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGACAAGGGCTGATCGGGTTTGTTCGTAAGGCGTATGACGTTAGTTTTTCGGCGCCTCT‑‑GA‑‑GATCGAGCGACGCGCGAGCGGCGATc > 8:149258/1‑149 (MQ=255) cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAt > 5:178654/1‑149 (MQ=255) cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTGTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAt < 5:246607/149‑1 (MQ=255) ctCCCGGCATGGATCGCTGGGCTGTGGGTGGCGTTCAACCCGAAGCAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtct < 7:199806/147‑1 (MQ=37) aTCCCGGCATGGATCGCTGTGCTGTGGGCGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtct < 3:70960/148‑1 (MQ=255) cccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtctga > 1:211422/1‑146 (MQ=255) cgggCATGGATCGCTGTGGTGTGGGTGGCTTTCAAGCTGATGAAGGCTCGCCAGGCAAAGGGCTGATCGGGTTTGTTCGTAAGGGTTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTTAGATCGCGCGCCGCGCGGGCGGCGCTCGATCTcac < 2:160729/146‑1 (MQ=38) aTCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATAGAGCGCCGCGCGGGCGGCGCTCGATCTAACAGGCGATGa > 2:155032/1‑148 (MQ=39) tCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGaaa < 1:327706/149‑1 (MQ=255) ctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAg < 6:178654/149‑1 (MQ=255) ctgtgGGTGGCGTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGGAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCCCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTaaa < 7:82194/148‑1 (MQ=38) ggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCAt < 2:67970/148‑1 (MQ=255) gtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGc > 2:235560/1‑149 (MQ=255) aaGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGAATGCGTCTggcgg > 7:141598/1‑149 (MQ=255) aGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTGCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggcggc < 2:317725/149‑1 (MQ=255) gCTGAAGCAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTACGGGGTTTGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGAGCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc < 7:283698/149‑1 (MQ=38) aagaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGa > 1:127315/1‑149 (MQ=255) cTCGCCAGGCCAACGGCTGCTCCGCGTAGGCCGTACGGGGTATGCCGTTAGGTTTTCGGCGCCTCT‑‑GA‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTc < 3:197147/149‑1 (MQ=25) tCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTcc > 2:95970/1‑149 (MQ=255) gCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAcgc > 3:281211/1‑142 (MQ=255) gCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAcgc < 4:281211/142‑1 (MQ=255) cAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTTGCGGCCCTGACGCGTTCCCCTTACATg > 7:173192/1‑149 (MQ=38) cTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTc < 8:141598/149‑1 (MQ=255) aTCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAt > 7:128239/1‑149 (MQ=255) tttGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGcc < 1:235560/149‑1 (MQ=255) gTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCt > 6:12220/1‑89 (MQ=21) gTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCt < 5:12220/89‑1 (MQ=21) gTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGcc > 7:85348/1‑148 (MQ=255) gggTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACATGCGATGAAAGCGTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgca > 1:184821/1‑148 (MQ=38) ccGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgt < 8:128239/149‑1 (MQ=255) cGTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg > 1:87417/1‑149 (MQ=255) gTTAGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtgg > 3:283717/1‑149 (MQ=255) aGGTTTTCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggtg < 5:45563/148‑1 (MQ=255) ggTTTTCGGCGC‑‑CT‑‑GTGAGAGCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTa < 8:64352/149‑1 (MQ=255) ttCGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAg < 8:85348/148‑1 (MQ=255) cGGCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCaa > 6:94827/1‑149 (MQ=255) ggCGC‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgc < 1:125323/113‑1 (MQ=255) gcgc‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAac < 5:217617/148‑1 (MQ=255) gcgc‑‑CT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTACCCTTCCATGCCCCTCAATTCTGCCTCCGCCAAGCACGCAAAGTGGTGTACACCAac > 6:217617/1‑148 (MQ=38) || TGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGC‑‑CT‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAAC > NC_002947/1070100‑1070384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |