| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,420,602:1 | +T | 12.7% | intergenic (+85/+22) | ctpH → / ← PP_t62 | methyl‑accepting chemotaxis protein CtpH/tRNA‑Pro |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,420,602 | 1 | . | T | 12.7% | 185.3 / 24.5 | 55 | intergenic (+85/+22) | ctpH/PP_t62 | methyl‑accepting chemotaxis protein CtpH/tRNA‑Pro |
| Reads supporting (aligned to +/- strand): ref base . (24/24); new base T (3/4); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
GGGGCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCTTGTTG > NC_002947/2420454‑2420748 | ggggCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATg < 2:143027/149‑1 (MQ=255) cgcCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCt < 4:109628/149‑1 (MQ=255) ccTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACGTG‑GCGGGCttt < 6:185367/149‑1 (MQ=255) ccTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCttt > 6:65221/1‑149 (MQ=255) ccTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCttt < 1:50137/149‑1 (MQ=255) gCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtgt > 5:170528/1‑148 (MQ=255) cATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtgt < 6:278932/147‑1 (MQ=255) cATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGa < 5:65221/149‑1 (MQ=255) tCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGAttt < 2:309256/149‑1 (MQ=255) aGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGga < 7:218870/149‑1 (MQ=255) aGTTGGGCGAAGTCACATCCCTCCTGAGTGCCTGACTCGGTCTGGAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCAGATG‑GCGGGCTTTGTGAGATTGTTTTGTTCGga < 4:309661/149‑1 (MQ=255) gCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAgg < 3:141115/132‑1 (MQ=255) gCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAgg > 4:141115/1‑132 (MQ=255) tCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGAGTc > 7:184416/1‑149 (MQ=255) cacaTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTc > 3:104876/1‑148 (MQ=255) aCCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCg > 3:128449/1‑148 (MQ=255) aCCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCg > 8:172543/1‑148 (MQ=255) ccTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCTCACACAACACAAAGCCCGCACATG‑GCGTGCTTTGTGAC‑AGGATTTGGTCGGAGAGACAGGATTCGAAc < 5:111805/149‑1 (MQ=255) cTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACa < 7:82659/139‑1 (MQ=255) cTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACa > 8:82659/1‑139 (MQ=255) ccGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtgt > 6:227216/1‑112 (MQ=255) ccGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGGGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtgt < 5:227216/112‑1 (MQ=255) cGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCACAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGc > 8:175301/1‑149 (MQ=255) gTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTtc < 8:71676/149‑1 (MQ=255) tCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCTTtgtg > 7:40430/1‑96 (MQ=38) tCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCTTtgtg < 8:40430/96‑1 (MQ=38) tCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACg > 4:232031/1‑148 (MQ=255) gCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGAc > 7:13266/1‑149 (MQ=255) gAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACgcgc > 3:188868/1‑149 (MQ=255) ttGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGAcg < 3:223188/143‑1 (MQ=255) ttGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGAcg > 4:223188/1‑143 (MQ=255) cAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCt < 8:52288/148‑1 (MQ=255) tGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTg < 4:188868/149‑1 (MQ=255) ccATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTgcg < 3:257867/149‑1 (MQ=255) ccATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTgc < 4:128449/148‑1 (MQ=255) gCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGTGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCt < 1:80259/134‑1 (MQ=255) gCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCt > 2:80259/1‑134 (MQ=255) gCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGa < 6:170528/149‑1 (MQ=255) cgcgCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAAc < 7:172543/149‑1 (MQ=255) aCGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTa > 7:194501/1‑149 (MQ=255) gTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACt < 7:175301/149‑1 (MQ=255) tttCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATc > 3:113685/1‑149 (MQ=255) cacaacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGtttt < 3:304498/149‑1 (MQ=255) acaactcaaTGCCCGCACATG‑GCTGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGTACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTAGCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGtttt < 2:34715/148‑1 (MQ=255) acaacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttttt > 3:154260/1‑149 (MQ=255) acaacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttttt > 3:149360/1‑149 (MQ=255) acaacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGtttt > 1:34715/1‑148 (MQ=255) aacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGc > 6:293763/1‑149 (MQ=255) cacaaAGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTc > 8:245541/1‑149 (MQ=255) acaaAGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttt > 6:272947/1‑142 (MQ=255) acaaAGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttt < 5:272947/142‑1 (MQ=255) aaaGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGc > 5:2660/1‑148 (MQ=255) aaaGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTAGc > 7:11661/1‑148 (MQ=255) aaGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCaa < 8:194501/149‑1 (MQ=255) cacaTG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCttg < 4:104876/149‑1 (MQ=255) aTG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCttgttg > 3:73200/1‑149 (MQ=255) | GGGGCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCTTGTTG > NC_002947/2420454‑2420748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |