Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 262,757:1 +C 10.1% coding (274/282 nt) PP_0211 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947262,7571.C10.1% 248.2 / 23.8 69coding (274/282 nt)PP_0211hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (31/31);  new base C (4/3);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

CGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACG  >  NC_002947/262611‑262905
                                                                                                                                                   |                                                                                                                                                    
cggcgCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑g                                                                                                                                                     <  7:34418/148‑1 (MQ=255)
  gcgcgcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTCACTTGC‑TGACCAGGTCacgcaa                                                                                                                                                  <  8:153583/149‑6 (MQ=255)
      gcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTg                                                                                                                                               >  4:83920/1‑148 (MQ=255)
      gcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGC‑TGACCAGGTCACGCAAGtaa                                                                                                                                              <  8:238673/149‑3 (MQ=255)
       ccTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGaa                                                                                                                                             <  2:156786/149‑1 (MQ=255)
       ccTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGC‑TGACCAGGTCACGCAAGtaag                                                                                                                                             <  2:309039/149‑4 (MQ=255)
        cTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAg                                                                                                                                            >  3:225747/1‑149 (MQ=255)
             cGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGAt                                                                                                                                       <  3:46236/149‑1 (MQ=255)
             cGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGAt                                                                                                                                       >  8:145529/1‑149 (MQ=255)
              tgAGGGATCAGCATGCTGCGCGACTATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGAATTGCGTACAACTGGTGATCAGCGATGGGCATGAGCGAGGGATCTACCATTCGGCTTACTTGCGTCACCTGGTCA‑GAAAGTGAAgataga                                                                                                                                       >  6:327517/2‑143 (MQ=255)
              ggAGGGATCAGCCTGGTGCGCGACTATGTGCGCGTGGTGCAAATCGAGTGGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGc                                                                                                                                           <  5:327517/144‑1 (MQ=255)
              ggAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATc                                                                                                                                      >  6:311697/1‑149 (MQ=255)
               gAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCt                                                                                                                                          <  4:97417/144‑1 (MQ=255)
               gAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCt                                                                                                                                          >  3:97417/1‑144 (MQ=255)
                    aTCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCa                                                                                                                                <  3:83920/149‑1 (MQ=255)
                       aGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGtt                                                                                                                             >  4:263650/1‑149 (MQ=255)
                       aGCCTGGTGCGCGACGATGTGCGCGGGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGtt                                                                                                                             <  8:56949/149‑1 (MQ=255)
                         ccTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGc                                                                                                                           >  2:309929/1‑149 (MQ=255)
                          cTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGGAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGcc                                                                                                                          <  3:74245/149‑1 (MQ=255)
                           tGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGtt                                                                                                                             >  7:66626/1‑145 (MQ=255)
                           tGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGtt                                                                                                                             <  8:66626/145‑1 (MQ=255)
                           tGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCg                                                                                                                         >  5:189601/1‑149 (MQ=255)
                              tGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGaa                                                                                                                      <  6:189601/149‑1 (MQ=255)
                              tGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGaa                                                                                                                      <  6:307307/149‑1 (MQ=255)
                                 gcgACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAggg                                                                                                                   <  1:156321/149‑1 (MQ=255)
                                   gacgaTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGt                                                                                                                 >  4:163769/1‑149 (MQ=255)
                                   gacgaTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGt                                                                                                                 <  4:279997/149‑1 (MQ=255)
                                       aTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAg                                                                                                             <  5:311697/149‑1 (MQ=255)
                                                 ggtgCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGgac                                                                                                    >  5:97482/1‑148 (MQ=255)
                                                  gtgCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGgacga                                                                                                  >  3:257240/1‑149 (MQ=255)
                                                    gCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAg                                                                                                                     >  7:276821/1‑128 (MQ=255)
                                                    gCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAg                                                                                                                     <  8:276821/128‑1 (MQ=255)
                                                    gCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTGGCCGGAAGGGGTGCAGAAGCAGGACGccc                                                                                                >  2:111233/1‑149 (MQ=255)
                                                     cAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGAcc                                                                                                <  6:313065/148‑1 (MQ=255)
                                                            aGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGcc                                                                                        <  3:163769/149‑1 (MQ=255)
                                                              tCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAg                                                                                                       >  3:213910/1‑132 (MQ=255)
                                                              tCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAg                                                                                                       <  4:213910/132‑1 (MQ=255)
                                                                gCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAAc                                                                                     >  6:257967/1‑148 (MQ=255)
                                                                 cAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCGTCAGTTGCCGGAAGGGGTGCAGAAGCAGGCCGACCGCCTCGCCGACg                                                                                    >  8:80396/1‑148 (MQ=255)
                                                                        aTGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGTCGGAATGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGcc                                                                            >  3:217755/1‑149 (MQ=255)
                                                                          ggCGGACCACTGGCGTGCAGCGATGGGCATGAGCGCGGGAGCTACCCTTGGGCTTACTTGCGGGACCTGGGCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGa                                                                          <  8:230375/149‑1 (MQ=255)
                                                                            cGTACTACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAgg                                                                        >  7:12696/1‑149 (MQ=255)
                                                                                 aaCTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCa                                                                                       >  1:157953/1‑129 (MQ=255)
                                                                                 aaCTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCa                                                                                       <  2:157953/129‑1 (MQ=255)
                                                                                      gtgtTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAggg                                                                                                                   >  2:241606/1‑96 (MQ=255)
                                                                                      gtgtTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAggg                                                                                                                   <  1:241606/96‑1 (MQ=255)
                                                                                      gtgtTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGc                                                               <  1:309929/148‑1 (MQ=255)
                                                                                       tgtTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCGGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGctc                                                             <  7:145529/149‑1 (MQ=255)
                                                                                            aGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTgg                                                        <  3:263650/149‑1 (MQ=255)
                                                                                             gCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGc                                                       <  6:97482/149‑1 (MQ=255)
                                                                                                         aGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGCCCGAGGGTAACCTGCTCTTTGGCGACACCCCGact                                           >  4:133167/1‑147 (MQ=255)
                                                                                                          gcgcGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAgt                                           <  7:80396/148‑1 (MQ=255)
                                                                                                            gcgGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGtgtg                                        <  4:225747/149‑1 (MQ=255)
                                                                                                              gggATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTggg                                      <  8:12696/149‑1 (MQ=255)
                                                                                                                  tCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTa                                    >  4:52875/1‑147 (MQ=255)
                                                                                                                       ccTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTgggagatcg                                >  5:51387/1‑140 (MQ=255)
                                                                                                                       ccTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTgg                                       <  6:51387/139‑1 (MQ=255)
                                                                                                                              ttcaCTTGC‑TGACCAGGTCACGCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTgg                      >  7:185927/4‑149 (MQ=255)
                                                                                                                              ttcaCTTGC‑TGACCAGGTCACGCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTgg                      >  5:7980/4‑149 (MQ=255)
                                                                                                                               tcaCTTGC‑TGACCAGGTCACGCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGTgg                                                                        >  1:266393/3‑98 (MQ=38)
                                                                                                                               tcaCTTGC‑TGACCAGGTCACGCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAgg                                                                        <  2:266393/96‑1 (MQ=38)
                                                                                                                                 aCTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGc                    >  7:29220/1‑148 (MQ=255)
                                                                                                                                   ttGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAg                                                                                                                     <  6:45081/49‑1 (MQ=255)
                                                                                                                                   ttGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAg                                                                                                                     >  5:45081/1‑49 (MQ=255)
                                                                                                                                     gCGTGACCTGGTCA‑TCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAAc                 <  4:257240/147‑1 (MQ=255)
                                                                                                                                     gCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAAc                 <  3:52875/147‑1 (MQ=255)
                                                                                                                                      gctGACCAGGTCACGCAAGT‑AAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGCCGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTg              >  6:274301/3‑149 (MQ=255)
                                                                                                                                      cGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGCCAACGTg              >  5:321202/1‑149 (MQ=255)
                                                                                                                                       gTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACAGGCCGAGGCTAACCTGCTCTTTGGCGACACCCAGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGc             >  7:297314/1‑149 (MQ=255)
                                                                                                                                              ggTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGAt      >  1:46202/1‑149 (MQ=255)
                                                                                                                                              ggTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGAt      >  8:208338/1‑149 (MQ=255)
                                                                                                                                                  a‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACg  <  8:29220/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACG  >  NC_002947/262611‑262905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑