| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 278,742 | 1 | . | A | 93.6% | 145.0 / 3.0 | 47 | intergenic (‑237/+77) | PP_0224/sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base A (21/23); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑AA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGC > NC_002947/278597‑278884 | gTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑aaa > 5:184400/1‑148 (MQ=255) gttACAGCTTTTGGCGAAATTCGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATTATTTATAA‑AATTTTTATTAAATAAGTTTTTTTATTATTTATAGATGGGGTTTTTGTTACAGAGGTGCCGGGCTCTTGGCGGG‑T‑AA‑acc < 2:101514/146‑1 (MQ=255) aCAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGc > 7:141829/1‑148 (MQ=255) aGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTcc < 3:178443/149‑1 (MQ=255) gCTGATCGCGACAGACGTGCGCTCGTCCGGTGGACCGGTATATAAACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTCGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCt < 3:24608/149‑1 (MQ=255) aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAGTATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTAAAGAGGTGACGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTac > 1:93699/1‑148 (MQ=255) aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTaca > 5:238593/1‑148 (MQ=255) cgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACAt < 2:205402/149‑1 (MQ=255) cgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACAt > 3:168109/1‑149 (MQ=255) gACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTc < 7:234751/149‑1 (MQ=255) tgCTGGTCCGGTGGTACGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATc > 4:115694/1‑149 (MQ=255) cTGGTCCGGTGGTCCGGTATATATACACTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGCG‑TAAA‑CCCGCTCCAACACACATGCCTCAAGATCtg > 6:142615/1‑149 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCtgt < 1:142827/147‑1 (MQ=255) tggtccggtATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa < 5:180338/149‑1 (MQ=255) tggtcaggtTTAAATTACCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTTGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa < 5:192674/149‑1 (MQ=255) gtccggtATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACCTGCCACAAGATCTGTGCAAGGTAg > 6:172769/1‑148 (MQ=255) cggtATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGc < 1:82929/149‑1 (MQ=255) cggtATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGc < 4:271336/149‑1 (MQ=255) gtATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCgg < 6:184400/149‑1 (MQ=255) gtATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCgg > 2:6724/1‑149 (MQ=255) gtATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCgg < 1:6724/149‑1 (MQ=255) gtATATATACCCTAATGATTTATAA‑AATTATTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCgg < 2:291047/149‑1 (MQ=255) tataCCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTa < 3:115694/149‑1 (MQ=255) taCCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTAcc < 4:168109/149‑1 (MQ=255) ccTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGACGGGCTCTTCGCGGG‑TAAA‑ACAGCTCCTACACACATGCCTCAAGATCTGTGCAATGTAGGAGCGGGTTTACCcg > 3:38326/1‑148 (MQ=255) cTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGc > 7:47065/1‑136 (MQ=255) cTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGc < 8:47065/136‑1 (MQ=255) cTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACAAGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCcgc > 2:93951/1‑148 (MQ=255) aTGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGa > 5:176745/1‑147 (MQ=255) tttAAAA‑AATTTTTATTAAATAAATTTTTGTAATATTTATATAAGGGGGATTTTTTACTGCGGGGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAATATCTGTCCACTGTAGGCGCGGTTTAACCCGCGAAAGGGc < 6:176745/149‑1 (MQ=25) ttATAA‑ATTTTTAATGAACTAACTTTTTGTAATATTTATAGACGGGGGATTTGTAACAGCGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCAACAACCAGGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAgg < 8:141829/146‑1 (MQ=255) a‑aaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCTCGAAAGGGCCGGaa > 7:71686/1‑148 (MQ=255) aaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGcc < 8:206885/143‑1 (MQ=255) aaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGcc > 7:206885/1‑143 (MQ=255) aaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGaa > 2:36351/1‑147 (MQ=255) aTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAg < 5:57007/149‑1 (MQ=255) tttttATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAgg < 6:238593/149‑1 (MQ=255) ggAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTT‑AA‑CCCGCACCAACACACCGGCCTCAAGCTCTGGGCACTGTAGGAGCGGGGTTACCCGCGAAAGGGCCCGAACCGGTTTACGCGGAGGCCGCCGTc > 6:289806/1‑149 (MQ=255) gAATATTTATAGAAGGGGGATTTGTTACCGAGGTGCCGGGCTCTTCGCGGG‑T‑AACCCCGCTCCTACACACATGCCTCACCATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTc < 5:172769/148‑1 (MQ=39) gAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCTGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGGGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTcc > 6:97762/1‑149 (MQ=255) gAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTcc > 2:72742/1‑149 (MQ=255) tatTTATAGAAGGGGGATTTGTTACAGAGGTGCCTGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACCCACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGa < 5:97762/149‑1 (MQ=255) tttATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAg > 4:96435/1‑148 (MQ=255) aaGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgc < 1:72742/149‑1 (MQ=255) agagGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTACTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcgcg > 4:251945/1‑148 (MQ=255) agGTGCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGCTCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAACGGCCGGCACAGGGTTACTCGGCGGCCGCAGGCCCGAGGAACATGCGCAAGAACTGCCGCGTAc > 3:48857/1‑149 (MQ=38) gCCGGGCTCTTCGCGGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTTCCCGCGAAAGGGCCGGAACAGGTTTACTCGGCGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGctc > 1:134678/1‑149 (MQ=255) gcgGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGTCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGgcgc < 2:93699/149‑1 (MQ=255) | GTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAA‑AATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑AA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGC > NC_002947/278597‑278884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |