Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	336,124:1	+T	100%	coding (209/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	98.0%	155.6 / ‑3.6	49	coding (209/237 nt)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (18/30);  total (18/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑T‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335976‑336263                                                                                                                                                      |                                                                                                                                              gTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGa                                                                                                                                                <  4:24108/149‑1 (MQ=255) gTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGa                                                                                                                                                >  2:224700/1‑149 (MQ=255)     aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttac                                                                                                                                           >  3:25281/1‑146 (MQ=255)      cccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacc                                                                                                                                          >  7:23085/1‑145 (MQ=255)       ccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                         >  1:98777/1‑144 (MQ=255)            ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTTGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGATGACGACCATAAATTCCTGGGGGCAGCTGATT‑ACCTGTGCg                                                                                                                                    <  8:280697/149‑1 (MQ=255)              tCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGa                                                                                                                                   >  2:173131/1‑148 (MQ=255)                 aTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGGGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATg                                                                                                                               <  6:79294/149‑1 (MQ=255)                  tctcCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATgg                                                                                                                              >  1:653/1‑149 (MQ=255)                      cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCaa                                                                                                                          <  2:134710/149‑1 (MQ=255)                      cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGAAGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCaa                                                                                                                          <  8:257306/149‑1 (MQ=255)                         cgcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAgg                                                                                                                        <  2:69224/148‑1 (MQ=255)                             tAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCtg                                                                                                                   <  4:122366/149‑1 (MQ=255)                              agagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCtga                                                                                                                  >  3:162009/1‑149 (MQ=255)                                agCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTTGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                                 >  3:194640/1‑148 (MQ=255)                                agCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCtgatg                                                                                                                <  8:23085/149‑1 (MQ=255)                                  cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGa‑a                                                                                                             <  1:224700/149‑1 (MQ=255)                                  cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGa‑a                                                                                                             >  3:144986/1‑149 (MQ=255)                                  cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGa‑a                                                                                                             >  1:46151/1‑149 (MQ=255)                                    cGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGgcgc                                                                                                                     <  5:269460/140‑1 (MQ=255)                                    cGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGgcgc                                                                                                                     >  6:269460/1‑140 (MQ=255)                                           cccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCg                                                                                                     <  5:186778/148‑1 (MQ=255)                                                    gcgGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGcc                                                                                             >  1:33755/1‑147 (MQ=255)                                                      ggCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAgg                                                                                         >  5:116001/1‑149 (MQ=255)                                                             gAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGgc                                                                                  <  2:46151/149‑1 (MQ=255)                                                             gAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGgc                                                                                  <  4:162009/149‑1 (MQ=255)                                                                 aTGTCTTGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                              <  4:194640/149‑1 (MQ=255)                                                                        gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAt                                                                       >  7:241393/1‑149 (MQ=255)                                                                          atgtGATGGGGTAAATGAAGCACCTGGTGGGGGATGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTg                                                                     <  3:271867/146‑1 (MQ=255)                                                                             tGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTCATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGAATTAGGCATTGAcg                                                                  >  2:205371/1‑149 (MQ=255)                                                                               aTGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACAATAAATTCGTGGGGGCAGCGGATT‑ACGTGTCCGACATGCCCAAGGCGGTGATCA‑AGGATAAGCAGAACCCAATGGGAAAGGCGAATAAGGCTTTGata                                                                  >  4:271867/1‑145 (MQ=255)                                                                                       cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtg                                                        >  3:177942/1‑149 (MQ=255)                                                                                        aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGCGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       <  1:205371/149‑1 (MQ=255)                                                                                        aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       <  7:74190/149‑1 (MQ=255)                                                                                         tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGATATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                      <  8:241393/149‑1 (MQ=255)                                                                                         tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                      <  2:98777/149‑1 (MQ=255)                                                                                          cAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAt                                                           <  7:216753/143‑1 (MQ=255)                                                                                          cAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAt                                                           >  8:216753/1‑143 (MQ=255)                                                                                           aaGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                     <  2:3985/148‑1 (MQ=255)                                                                                                ccTGGGGCGCGTGGGCTAAATGCAGCACCACAATAAATTCCTGGGGGCAGCCTATT‑ACCGGTGCCAAATGCCAAAGCGGCTCATCACCCGTTATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCg                                                <  1:93343/149‑1 (MQ=255)                                                                                                              gCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGaa                                 <  2:33755/149‑1 (MQ=255)                                                                                                               cGATATGGAGGACGACCATAAAATCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                                >  7:201732/1‑149 (MQ=255)                                                                                                                  tatGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATggg                             <  1:173131/149‑1 (MQ=255)                                                                                                                      caggaCGACCATAAATTCCTGCGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTg                          <  5:109948/147‑1 (MQ=255)                                                                                                                            gacCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagca                   <  6:116001/149‑1 (MQ=255)                                                                                                                            gacCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagca                   <  2:80708/149‑1 (MQ=255)                                                                                                                              ccATTAATTCGTGGGGGCAGCCGATT‑ACCTGTGCGACATTGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCTTTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAag                     <  4:198835/145‑1 (MQ=255)                                                                                                                              ccATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagcaca                 >  3:198835/1‑147 (MQ=255)                                                                                                                               cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGcc                >  1:317035/1‑149 (MQ=255)                                                                                                                                ctAAATTCCTGGGGGCCGCCGA‑TCACCTGTGCCACATGGCCACGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGccc               <  4:25281/148‑1 (MQ=39)                                                                                                                                aTAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGccc               <  4:177942/149‑1 (MQ=255)                                                                                                                                    aTTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCTTTGCAGcccattc           <  7:73189/149‑5 (MQ=39)                                                                                                                                             ggCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  3:19829/149‑1 (MQ=255)                                                                                                                                               cAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTTCGTAGCGAAATGGCTGCAAAGCAGCCCCCGCGAtttaa    >  7:12417/1‑143 (MQ=255)                                                                                                                                                      |                                                                                                                                              GTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑T‑ACCTGTGCGACATGGCCAAGGCGCTGATGA‑ACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335976‑336263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑