Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,183,249	C→G	5.6%	G162A (GGC→GCC)	PP_4563 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,183,249	0	C	G	5.6%	233.0 / 2.8	71	G162A (GGC→GCC)	PP_4563	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (37/30);  new base G (2/2);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

TACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGCACCACGCAGTAAT  >  NC_002947/5183104‑5183396                                                                                                                                                  |                                                                                                                                                    tACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCa                                                                                                                                                  >  4:256455/1‑149 (MQ=255)   ccGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGccc                                                                                                                                                   <  5:305563/146‑1 (MQ=255)   ccGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGccc                                                                                                                                                   >  6:305563/1‑146 (MQ=255)       cTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGGCTTCAAGGCCATcatca                                                                                                                                            <  3:29347/149‑5 (MQ=255)       cTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGGCTTCAAGGCCATcatca                                                                                                                                            <  3:33395/149‑5 (MQ=255)           aGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTc                                                                                                                                        <  6:230387/149‑1 (MQ=255)             gCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGc                                                                                                                                      >  8:150045/1‑149 (MQ=255)               tCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCg                                                                                                                                    >  7:40133/1‑149 (MQ=255)                 aGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCg                                                                                                                                    <  8:10502/147‑1 (MQ=255)                 aGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCg                                                                                                                                    <  3:168871/147‑1 (MQ=255)                  gTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGttt                                                                                                                                         <  1:323468/141‑1 (MQ=255)                  gTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGttt                                                                                                                                         >  2:323468/1‑141 (MQ=255)                   tGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGcca                                                                                                                                >  2:123081/1‑149 (MQ=255)                        tCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACcag                                                                                                                            >  7:265340/1‑148 (MQ=255)                          ttGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACcagcag                                                                                                                         >  7:224783/1‑149 (MQ=255)                            gCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCt                                                                                                                       >  3:53753/1‑149 (MQ=255)                                tCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGAc                                                                                                                   <  3:256455/149‑1 (MQ=255)                                tCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGAc                                                                                                                   <  1:123081/149‑1 (MQ=255)                                     gcgcACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCt                                                                                                              >  6:229934/1‑149 (MQ=255)                                         aCTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTc                                                                                                                                        <  7:211198/119‑1 (MQ=255)                                         aCTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTc                                                                                                                                        >  8:211198/1‑119 (MQ=255)                                               ttGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCAACCAGCAGCTCGACCGGCTGGCCAAACtt                                                                                                    >  1:73271/1‑149 (MQ=255)                                                 gatgaAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTg                                                                                                   <  8:40133/148‑1 (MQ=255)                                                 gatgaAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTg                                                                                                   <  7:150045/148‑1 (MQ=255)                                                     aaGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGGGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGt                                                                                               <  7:134846/148‑1 (MQ=255)                                                      aGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGccacca                                                                                                                             <  3:153390/117‑1 (MQ=255)                                                      aGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGccacca                                                                                                                             >  4:153390/1‑117 (MQ=255)                                                          cTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCGTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCg                                                                                         >  6:324226/1‑149 (MQ=255)                                                            gctcGGGCATGCACGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTg                                                                                                   <  2:323442/136‑1 (MQ=255)                                                            gctcGGGCATGCACGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTg                                                                                                   >  1:323442/2‑137 (MQ=255)                                                             ctcGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCggg                                                                                       <  5:229934/148‑1 (MQ=255)                                                                gTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGATTCGCCGGCCACCAGCAGCTCGACCGCCTGGCCAACCATGTAGTCGGCCGGGCTGa                                                                                   >  2:257517/1‑149 (MQ=255)                                                                    aTGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATc                                                                               >  6:112678/1‑149 (MQ=255)                                                                        ccGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGccac                                                                                                                               <  3:93070/97‑1 (MQ=255)                                                                        ccGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGccac                                                                                                                               >  4:93070/1‑97 (MQ=255)                                                                        ccGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAgg                                                                           >  1:113361/1‑149 (MQ=255)                                                                        ccGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAgg                                                                           >  6:123053/1‑149 (MQ=255)                                                                          gAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTa                                                                         <  5:112678/149‑1 (MQ=255)                                                                                 aGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTc                                                                   >  3:316261/1‑148 (MQ=255)                                                                                  gAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTc                                                                   >  5:325080/1‑147 (MQ=255)                                                                                    aCTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAg                                                                <  6:264091/148‑1 (MQ=255)                                                                                      ttGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGgcg                                                             >  1:250771/1‑149 (MQ=255)                                                                                        gAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGgcg                                                             <  2:250771/147‑1 (MQ=255)                                                                                            acCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGt                                                       >  4:172095/1‑149 (MQ=255)                                                                                              cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGTCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTgg                                                     >  6:172183/1‑149 (MQ=255)                                                                                              cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGgc                                                              <  8:168813/140‑1 (MQ=255)                                                                                              cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGgc                                                              >  7:168813/1‑140 (MQ=255)                                                                                              cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTgg                                                     <  7:27238/149‑1 (MQ=255)                                                                                              cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTgg                                                     >  7:217492/1‑149 (MQ=255)                                                                                                cGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCa                                                   <  5:123053/149‑1 (MQ=255)                                                                                                       gtgGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGa                                               <  4:316261/146‑1 (MQ=255)                                                                                                          gATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGTCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCcggc                                         <  5:172183/149‑1 (MQ=255)                                                                                                              aGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCggg                                      <  8:224783/148‑1 (MQ=255)                                                                                                                 ccccAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTgcg                                  >  3:212390/1‑149 (MQ=255)                                                                                                                 ccccAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTgcg                                  >  4:85319/1‑149 (MQ=255)                                                                                                                   ccAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCt                                >  5:29199/1‑149 (MQ=255)                                                                                                                      gTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGt                             <  4:53753/149‑1 (MQ=255)                                                                                                                         gCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGa                          <  6:29199/149‑1 (MQ=255)                                                                                                                          cGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCTGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAg                         <  6:77107/149‑1 (MQ=255)                                                                                                                            gttgttGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCAACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAg                                                                            >  2:36476/1‑96 (MQ=255)                                                                                                                            gttgttGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCAACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAg                                                                            <  1:36476/96‑1 (MQ=255)                                                                                                                              tgttgATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTa                      >  5:140794/1‑148 (MQ=255)                                                                                                                               gttgATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTaca                    >  8:256858/1‑149 (MQ=255)                                                                                                                                   ccgatgGGCTTCAAGGCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTg                >  1:194390/3‑149 (MQ=255)                                                                                                                                   ccgatgGGCTTCAAGGCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTg                >  1:189656/3‑149 (MQ=255)                                                                                                                                    tgatgGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACTTCGAGGTCGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGc               <  1:257517/149‑1 (MQ=255)                                                                                                                                     gatgGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGca              >  1:309463/1‑149 (MQ=255)                                                                                                                                     gatgGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGc               <  8:265340/148‑1 (MQ=255)                                                                                                                                     gatgGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGACGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGca              >  4:222996/1‑149 (MQ=255)                                                                                                                                          ccTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGCACCACg         >  1:9366/1‑149 (MQ=255)                                                                                                                                                aGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGCACCACGCAGTa    <  6:325080/148‑1 (MQ=255)                                                                                                                                                 gCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGCACCACGCAGTAAt  >  2:210932/1‑149 (MQ=255)                                                                                                                                                 gCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGCACCACGCAGTAAt  >  8:25743/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGCACCACGCAGTAAT  >  NC_002947/5183104‑5183396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑