| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,186,053 | T→G | 10.2% | E136A (GAG→GCG) | aceK ← | isocitrate dehydrogenase kinase/phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,186,053 | 0 | T | G | 10.2% | 149.9 / 6.4 | 56 | E136A (GAG→GCG) | aceK | isocitrate dehydrogenase kinase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (22/28); new base G (4/2); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCATTCGCGCAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCGGGGGTCGAT > NC_002947/5185906‑5186186 | aggcaTTCGCGCAGTTGTTCTTCCAGCCGCGCCAGGCCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTc > 5:214631/1‑149 (MQ=255) aTTCGCGCAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTAAGGGTTTGCGCGG‑CGAGCTcgctg > 8:125882/1‑147 (MQ=255) gcgcAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCcgc < 6:247006/149‑1 (MQ=255) gcAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCCTGTCGAACGGGTACTCGGCGAACACCGCGCGCTGCAAGTTCCTGAGACTGCCGGCAAGCCGATAGGTAGGGGTTTGCGCGG‑CGCGCGCGCG‑GCcgcgg > 4:266759/1‑148 (MQ=255) gttCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATgg > 3:314622/1‑147 (MQ=255) gttCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATgg > 7:172791/1‑147 (MQ=255) cttcCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGAGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGc > 5:215788/1‑149 (MQ=255) cAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCgg < 3:241600/148‑1 (MQ=255) cAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCgg < 7:182007/148‑1 (MQ=255) aGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGTGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑C‑CGCGAGCGCGCCGCGCa > 3:295597/1‑136 (MQ=255) aGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGTGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑C‑CGCGAGCGCGCCGCGCa < 4:295597/136‑1 (MQ=255) aGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCggg > 6:158655/1‑148 (MQ=255) aGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCggg > 3:304534/1‑148 (MQ=255) cgcgCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAgt < 4:314622/149‑1 (MQ=255) gcCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGtgtg < 6:117740/149‑1 (MQ=255) ccAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGtgtg > 4:126542/1‑148 (MQ=255) ccAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTgg < 3:292791/149‑1 (MQ=255) cAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGa < 3:226079/149‑1 (MQ=255) aGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGAt > 4:132965/1‑149 (MQ=255) ccTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAAc > 5:142598/1‑147 (MQ=255) ccTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAAc < 6:142598/147‑1 (MQ=255) ccTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACAt < 4:304534/149‑1 (MQ=255) ccTTCCAGGGCGCCATAGGGCATGTCGAAGGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACTGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACAt < 8:235112/149‑1 (MQ=255) ttCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCCG‑CGCGCGAGCG‑GCCGCGCATGGAAGGCCCGGGAGTGTGGATGAACATg > 2:310682/1‑148 (MQ=255) ccAGGTCGCCATAGCGCATGGCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTATGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCa < 8:220672/148‑1 (MQ=255) ggTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCg > 2:184950/1‑149 (MQ=255) gCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCg < 3:126542/148‑1 (MQ=255) gCCATAGGGCATGTCGAACGGGTAAGCGTCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGGCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGATGGCCGGGTAGTGTGGATGAACATGCAGCCGTCg < 7:125882/148‑1 (MQ=255) cATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTgg < 8:84662/128‑1 (MQ=255) cATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTgg > 7:84662/1‑128 (MQ=255) tAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGAt > 7:176232/1‑149 (MQ=255) tAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGAt < 6:215788/149‑1 (MQ=255) ggCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAg < 5:101944/149‑1 (MQ=255) ggCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAg < 8:176232/149‑1 (MQ=255) aTGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTc < 1:184950/149‑1 (MQ=255) cGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATgg < 8:172791/149‑1 (MQ=255) aaCGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCt > 1:12119/1‑149 (MQ=255) cGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTg > 5:108263/1‑148 (MQ=255) aTCTGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAgg < 1:310682/148‑1 (MQ=255) gAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCaa > 8:97054/1‑148 (MQ=255) acacCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCaaaa > 4:98566/1‑148 (MQ=255) acacCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAAc < 3:179064/149‑1 (MQ=255) gcgcgcAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTTCGCGT‑CGCGCTCGCG‑GCCGCTCATGGAAGTCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGa < 5:158655/149‑1 (MQ=255) gcagcaAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGtcg < 7:97054/149‑3 (MQ=255) cagcaAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTg > 3:234781/1‑148 (MQ=255) cagcaAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTg > 3:291674/1‑148 (MQ=255) gcaAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTAc < 3:132965/149‑1 (MQ=255) gcaAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTAc < 6:214631/149‑1 (MQ=255) tGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTctct > 1:68936/1‑149 (MQ=255) agaCTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTctct < 2:12119/147‑1 (MQ=255) tCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACtcgtc < 3:258227/149‑1 (MQ=255) gATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAgg < 4:291674/149‑1 (MQ=255) gggTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCGGGGGTc > 1:48904/1‑148 (MQ=255) tttGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCGGGGGTCGAt > 5:114721/1‑148 (MQ=255) tGCGCGGCCGCGAGCGC‑‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCg < 6:142572/137‑1 (MQ=255) tGCGCGGCCGCGAGCGC‑‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCg > 5:142572/1‑137 (MQ=255) | AGGCATTCGCGCAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGG‑CGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCGGGGGTCGAT > NC_002947/5185906‑5186186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |