Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,194,887 T→G 9.2% intergenic (+29/+24) PP_4574 → / ← PP_4575 hypothetical protein/urea amidolyase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,194,8870TG 9.2% 120.6 / 2.1 50intergenic (+29/+24)PP_4574/PP_4575hypothetical protein/urea amidolyase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base G (2/3);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCG  >  NC_002947/5194739‑5195035
                                                                                                                                                    |                                                                                                                                                    
cccAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCt                                                                                                                                                      >  1:24941/1‑149 (MQ=255)
accAAGCAGGACTGGGACTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAACATGTATCCGCTCACCCTGCAAGGGTTCGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTATCGCGGGCt                                                                                                                                                      >  6:3716/2‑149 (MQ=255)
           cTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTTTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                           <  6:160524/149‑1 (MQ=255)
              ggAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAgg                                                                                                                                         <  2:93154/148‑1 (MQ=255)
              ggAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAg                                                                                                                                          <  1:229705/147‑1 (MQ=255)
                         ccTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGCGCCCGCGAAAGGGCCAGCAGTCtt                                                                                                                             <  8:193732/149‑1 (MQ=255)
                             gACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGa                                                                                                                         <  2:24941/149‑1 (MQ=255)
                              agacATCTGGTGCGCGACCTGATCCAGTACATGATGTATCCGCTGACCCTGCAAGGGTTGGAGGAGGGGAGGGGCTACCTGTCCATGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCATCAGTCTTACGAc                                                                                                                        <  5:3716/146‑1 (MQ=255)
                                ccATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGAcc                                                                                                                       >  3:152641/1‑148 (MQ=255)
                                         gcgtGACTTTATCGTGGACAAGATTTATCCGTTGACGCTGCAAGGGTTGGATGTCGGGTTGCGCTAGCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCTGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                             <  7:295739/149‑1 (MQ=255)
                                         gcgcGACCTGATCGAGTCCAAGAGGTATCCGCTGCCCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGACCAAGTGCGGTTGCCTGTACCGGCCCTTTCGCGGGCGCGCCCGCTAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                             <  6:221342/149‑1 (MQ=255)
                                         gcgcGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                             <  6:78507/149‑1 (MQ=255)
                                               ccTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAg                                                                                                       <  4:152641/149‑1 (MQ=255)
                                                   aTCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctgc                                                                                                   >  8:9536/1‑149 (MQ=255)
                                                     cGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctgctg                                                                                                 >  8:3267/1‑149 (MQ=255)
                                                          aCAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCtctc                                                                                            >  1:210284/1‑149 (MQ=255)
                                                            aaGATGTATCCGCTGACCCTGAAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGc                                                                                          >  6:304916/1‑149 (MQ=255)
                                                                    tCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTc                                                                                  >  8:97102/1‑149 (MQ=255)
                                                                    tCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTc                                                                                  >  2:137691/1‑149 (MQ=255)
                                                                    tCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGAGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGATGCTGCTCTCGCCAGGCCTc                                                                                  >  3:221205/1‑149 (MQ=255)
                                                                      cGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                             >  6:86193/1‑120 (MQ=255)
                                                                      cGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                             <  5:86193/120‑1 (MQ=255)
                                                                      cGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGGCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGt                                                                                <  2:240408/149‑1 (MQ=255)
                                                                            cccTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGca                                                                          >  5:73400/1‑149 (MQ=255)
                                                                             ccTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGcac                                                                         >  2:183638/1‑149 (MQ=255)
                                                                                      ggtGGAGCTCGGGATGCGCAACCTGCCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCCGGTAAAGCTGCTGCTCGCGCCAGGCCTCGTCCTGCACCACCGCCCt                                                                <  2:240963/147‑1 (MQ=255)
                                                                                          gaggaCGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGaa                                                             <  3:262846/148‑1 (MQ=255)
                                                                                                gggATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGcacc                                                      <  7:3267/149‑1 (MQ=255)
                                                                                                gggATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGcac                                                       >  2:20108/1‑148 (MQ=255)
                                                                                                        cTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGTACCACCGCCCTGAACCGCACCACCGTccc                                              <  3:64963/149‑1 (MQ=255)
                                                                                                               tCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCAt                                        <  1:20108/148‑1 (MQ=255)
                                                                                                               tCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATg                                       >  7:227006/1‑149 (MQ=255)
                                                                                                                  acgtgtGGGTGCCGGGACCGGCCCGTTCGCGGGCTCGCCCGCGAAAGGGCCAGCATGCTTACGACCAACGCCCCATGTAAAGCTGCTGCTCTCGCCATGCCTCGTCCTGCAACACCGCCCTGAACCGCACCACCGTCCCCg                                            <  4:156471/139‑1 (MQ=255)
                                                                                                                  aaGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGTTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTAATCTCGCCAGGCATCATCCTGCACCACCCCCCTGAACCGCACCACCGTCCCCAGCATTCa                                     >  8:36177/1‑148 (MQ=255)
                                                                                                                  aaGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCCCCACCGCCCTGAACCGCACCACCGTCCCCg                                            >  3:156471/1‑141 (MQ=255)
                                                                                                                  aaGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCggacatcg                                     >  3:151502/1‑142 (MQ=255)
                                                                                                                  aaGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCg                                            <  4:151502/141‑1 (MQ=255)
                                                                                                                     tGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGGAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCCCCACCGCCCTGACCCGCACCACCGTCCCCGGcctgccctgc                                 >  8:33018/1‑149 (MQ=255)
                                                                                                                         gTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTgcgc                               >  2:68072/1‑147 (MQ=255)
                                                                                                                         gTTGCCTGTACCGGCCATTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCa                             <  2:276352/149‑1 (MQ=255)
                                                                                                                           tGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAACGGGCCCGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGt                           >  5:100811/1‑149 (MQ=255)
                                                                                                                                  aCCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCa                             <  8:229415/140‑1 (MQ=255)
                                                                                                                                  aCCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCa                             >  7:229415/1‑140 (MQ=255)
                                                                                                                                      gCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGCCCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCGGCGTCCTGCACCACCGCCCGGCACCGCCCCCCCGTCCCCGGCCTGCACTGCGCCAGTTgcgccagcgc                >  3:170480/1‑149 (MQ=255)
                                                                                                                                      gCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgc                <  7:97102/149‑1 (MQ=255)
                                                                                                                                      gCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgc                >  2:208338/1‑149 (MQ=255)
                                                                                                                                        ccTTTCGCGGGCGAGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgc                >  1:45671/1‑147 (MQ=255)
                                                                                                                                          tttCGCGGGCGAGCCCGCGAAAGGGCCGGCAGTCTTACGACCAACACCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCgccagcgccagc            >  7:281498/1‑149 (MQ=255)
                                                                                                                                            tCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCgccagcgccagc            <  7:9536/147‑1 (MQ=255)
                                                                                                                                            tCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg           <  5:304916/148‑1 (MQ=255)
                                                                                                                                             cgcgGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAgcggc         >  1:273547/1‑149 (MQ=255)
                                                                                                                                               cgGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGt       <  6:73400/149‑1 (MQ=255)
                                                                                                                                                    tCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAgcg  >  5:19499/1‑149 (MQ=255)
                                                                                                                                                    tCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAgcg  >  3:30148/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCG  >  NC_002947/5194739‑5195035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑