Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,269,314	A→T	6.6%	L342H (CTC→CAC) ‡	radA ←	DNA repair enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,269,314	0	A	T	6.6%	227.1 / 5.0	76	L342H (CTC→CAC) ‡	radA	DNA repair enzyme
Reads supporting (aligned to +/- strand):  ref base A (31/39);  new base T (3/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

CAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCAC  >  NC_002947/5269166‑5269453                                                                                                                                                     |                                                                                                                                             cAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGa                                                                                                                                              <  4:203207/149‑1 (MQ=255) cAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGa                                                                                                                                              <  3:317166/149‑1 (MQ=255)  aaaCCATGGGCCCGTGGCCGGTTGCGCAAGCTCGACTTCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAg                                                                                                                                             <  5:100963/149‑1 (MQ=255)  aaaCCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAg                                                                                                                                             <  8:31429/149‑1 (MQ=255)      cATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCAcc                                                                                                                                         <  2:88177/149‑1 (MQ=255)           gCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaa                                                                                                                                    <  4:88518/149‑1 (MQ=255)           gCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGGTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaa                                                                                                                                    >  3:203660/1‑149 (MQ=255)             cAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaac‑a                                                                                                                                 <  8:160333/149‑1 (MQ=255)             cAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaac‑a                                                                                                                                 <  3:103548/149‑1 (MQ=255)              aGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGCTCCACCGCcatggc                                                                                                                                 <  6:102626/149‑5 (MQ=255)                     ggTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGcc                                                                                                                         <  6:182680/149‑1 (MQ=255)                      gTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCa                                                                                                                        <  5:58560/149‑1 (MQ=255)                           gcAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTAATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACgg                                                                                                                   >  7:202541/1‑149 (MQ=255)                            cAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGt                                                                                                                  >  8:197461/1‑149 (MQ=255)                             aaGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGt                                                                                                                  >  8:39695/1‑148 (MQ=255)                               gCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCt                                                                                                               <  8:175332/149‑1 (MQ=255)                                 tCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGAACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGCGATACCGCCATGCCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTACTg                                                                                                              >  4:1051/1‑148 (MQ=255)                                      aTCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAg                                                                                                        <  2:295699/149‑1 (MQ=255)                                      aTCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAg                                                                                                        <  6:294351/149‑1 (MQ=255)                                        cACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGc                                                                                                      >  4:97403/1‑149 (MQ=255)                                            gccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGAACCGCCAAC‑AACATGGAAATACGGTTATGGTCAAGGCACaa                                                                                                  >  2:132251/1‑149 (MQ=255)                                             ccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAg                                                                                                 >  4:220947/1‑149 (MQ=255)                                             ccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAg                                                                                                 <  3:263075/149‑1 (MQ=255)                                              cgccAGCAGGGTCTTGTCCGAATCCTTTTCCAGCTCCTTCACCCCTCCGTCAACGTTTATAAACACATCCTTTTCATTTGTCGGGTTACCGTTATGGCTGTGGAGCACCGTCAAC‑AATATGGCCAGACGGTTCTGGTCCAGGCCCAAgg                                                                                                <  8:202541/149‑1 (MQ=255)                                              cgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAgg                                                                                                <  1:97982/149‑1 (MQ=255)                                                ccAGCAGGGCCAGGTCCGAAGCCGGTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGtt                                                                                              <  8:121395/149‑1 (MQ=255)                                                 atgcagGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGTGAATCACATCCTGGTCATGTGTCGGGATAGCGCCATGGCGGTGGAGCAGCTACAAC‑AACATGGCCAGACGGTTCTGGTCGAGTCCCAAGGTTa                                                                                             <  8:27347/147‑1 (MQ=255)                                                     agggccagggccGAAGCCGTTTCCAGCACCTTCACCCCGCCGAGCACGTTGAGAAACACATCCTGTTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACccg                                                                                         <  2:240191/149‑1 (MQ=255)                                                       ggCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACccgcc                                                                                       >  7:32869/1‑149 (MQ=255)                                                          cAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCcgcg                                                                                    >  2:110278/1‑149 (MQ=255)                                                              tCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGtt                                                                                <  3:97403/149‑1 (MQ=255)                                                              tCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGtt                                                                                <  1:110278/149‑1 (MQ=255)                                                                cGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGCGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTgg                                                                              >  6:48880/1‑149 (MQ=255)                                                                 gAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGc                                                                             >  1:302427/1‑149 (MQ=255)                                                                  aaGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGc                                                                             >  7:171611/1‑148 (MQ=255)                                                                       gTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCgg                                                                                   <  2:112800/137‑1 (MQ=255)                                                                       gTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTgggggcggg                                                                        >  1:112800/1‑142 (MQ=255)                                                                        tttCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATgg                                                                      >  6:329119/1‑149 (MQ=255)                                                                          tCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCATCTAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATgg                                                                      <  1:93120/148‑1 (MQ=255)                                                                            cAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCAcc                                                                                                                                         <  6:323566/79‑1 (MQ=255)                                                                            cAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCAcc                                                                                                                                         >  5:323566/1‑79 (MQ=255)                                                                            cAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGtt                                                                                                                 <  3:203524/102‑1 (MQ=255)                                                                            cAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGtt                                                                                                                 >  4:203524/1‑102 (MQ=255)                                                                                 ccTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTAtcgtcg                                                             <  3:220947/149‑1 (MQ=255)                                                                                      aCCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAg                                                        >  2:192726/1‑149 (MQ=255)                                                                                       ccccGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgc                                                       <  1:132251/149‑1 (MQ=255)                                                                                         ccgccgACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgcgc                                                     <  4:178388/149‑1 (MQ=255)                                                                                            ccgACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTg                                                  <  2:302427/149‑1 (MQ=255)                                                                                               aCCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCAc                                               >  7:99731/1‑149 (MQ=255)                                                                                                ccACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTAtcgtc                                                              <  1:270174/133‑1 (MQ=255)                                                                                                ccACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTAtcgtc                                                              >  2:270174/1‑133 (MQ=255)                                                                                                 cACGTTCAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCt                                             <  1:192726/149‑1 (MQ=255)                                                                                                  aCGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTc                                            <  7:39695/149‑1 (MQ=255)                                                                                                  aCGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTc                                            >  1:168931/1‑149 (MQ=255)                                                                                                    gTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGCTGGCTTTCGTCGACCAGCGCGTGCACCGCGa                                          >  6:219242/1‑149 (MQ=255)                                                                                                          aaaCACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCa                                    >  6:5407/1‑149 (MQ=255)                                                                                                            acacATCCTGCTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCAtt                                  <  6:268286/149‑1 (MQ=255)                                                                                                                aTCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAgg                                                                                                <  6:110212/83‑1 (MQ=255)                                                                                                                aTCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAgg                                                                                                >  5:110212/1‑83 (MQ=255)                                                                                                                 tCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCg                             <  5:5407/149‑1 (MQ=255)                                                                                                                      gTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTa                         <  7:197461/148‑1 (MQ=255)                                                                                                                         aTGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgcgc                                                     >  6:92680/1‑117 (MQ=255)                                                                                                                         aTGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgcgc                                                     <  5:92680/117‑1 (MQ=255)                                                                                                                          tGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTc                    >  5:258889/1‑149 (MQ=255)                                                                                                                             gTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCCCCGCGACCAGCATTGGCCGGGTAGCCTcc                   >  3:65661/1‑147 (MQ=255)                                                                                                                             gTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCa                 <  8:32869/149‑1 (MQ=255)                                                                                                                                gggATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGCCCAGCATTGGCCGGGTACCCTCCCAGGt              >  7:170471/1‑149 (MQ=255)                                                                                                                                gggATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGt              >  2:143043/1‑149 (MQ=255)                                                                                                                                  gATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTg            <  8:99731/149‑1 (MQ=255)                                                                                                                                  gATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTg            >  6:144562/1‑149 (MQ=255)                                                                                                                                      ccGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCAt        <  2:168931/149‑1 (MQ=255)                                                                                                                                       cGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGCCCAGCATTGGCCGGGTACCCTCCCAGGTTGCCAt        >  4:131804/1‑148 (MQ=255)                                                                                                                                        ttgttGGCGGTGCTCCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCCCCCAGGTTGCCATca      >  8:224995/5‑149 (MQ=255)                                                                                                                                           ttGGCGGTGCTCCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCgg                            <  6:185751/123‑1 (MQ=255)                                                                                                                                           ttGGCGGTGCTCCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCgg                            >  5:185751/2‑124 (MQ=255)                                                                                                                                           ttGGCGGTGCTCCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcacca   >  6:145230/2‑149 (MQ=255)                                                                                                                                           aTGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcacca   >  5:217066/1‑149 (MQ=255)                                                                                                                                            tGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcaccac  <  8:171611/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                             CAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCAC  >  NC_002947/5269166‑5269453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑