Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,269,315 G→C 6.8% L342V (CTC→GTC) ‡ radA ← DNA repair enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,269,3150GC 6.8% 241.5 / 6.7 74L342V (CTC→GTC) ‡radADNA repair enzyme
Reads supporting (aligned to +/- strand):  ref base G (32/37);  new base C (3/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

AAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGCTACCCGGC  >  NC_002947/5269167‑5269463
                                                                                                                                                    |                                                                                                                                                     
aaaCCATGGGCCCGTGGCCGGTTGCGCAAGCTCGACTTCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAg                                                                                                                                                       <  5:100963/149‑1 (MQ=255)
aaaCCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAg                                                                                                                                                       <  8:31429/149‑1 (MQ=255)
    cATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCAcc                                                                                                                                                   <  2:88177/149‑1 (MQ=255)
         gCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaa                                                                                                                                              <  4:88518/149‑1 (MQ=255)
         gCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGGTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaa                                                                                                                                              >  3:203660/1‑149 (MQ=255)
           cAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaac‑a                                                                                                                                           <  3:103548/149‑1 (MQ=255)
           cAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaac‑a                                                                                                                                           <  8:160333/149‑1 (MQ=255)
            aGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGCTCCACCGCcatggc                                                                                                                                           <  6:102626/149‑5 (MQ=255)
                   ggTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGcc                                                                                                                                   <  6:182680/149‑1 (MQ=255)
                    gTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCa                                                                                                                                  <  5:58560/149‑1 (MQ=255)
                         gcAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTAATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACgg                                                                                                                             >  7:202541/1‑149 (MQ=255)
                          cAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGt                                                                                                                            >  8:197461/1‑149 (MQ=255)
                           aaGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGt                                                                                                                            >  8:39695/1‑148 (MQ=255)
                             gCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCt                                                                                                                         <  8:175332/149‑1 (MQ=255)
                               tCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGAACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGCGATACCGCCATGCCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTACTg                                                                                                                        >  4:1051/1‑148 (MQ=255)
                                    aTCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAg                                                                                                                  <  2:295699/149‑1 (MQ=255)
                                    aTCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAg                                                                                                                  <  6:294351/149‑1 (MQ=255)
                                      cACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGc                                                                                                                >  4:97403/1‑149 (MQ=255)
                                          gccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGAACCGCCAAC‑AACATGGAAATACGGTTATGGTCAAGGCACaa                                                                                                            >  2:132251/1‑149 (MQ=255)
                                           ccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAg                                                                                                           <  3:263075/149‑1 (MQ=255)
                                           ccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAg                                                                                                           >  4:220947/1‑149 (MQ=255)
                                            cgccAGCAGGGTCTTGTCCGAATCCTTTTCCAGCTCCTTCACCCCTCCGTCAACGTTTATAAACACATCCTTTTCATTTGTCGGGTTACCGTTATGGCTGTGGAGCACCGTCAAC‑AATATGGCCAGACGGTTCTGGTCCAGGCCCAAgg                                                                                                          <  8:202541/149‑1 (MQ=255)
                                            cgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAgg                                                                                                          <  1:97982/149‑1 (MQ=255)
                                              ccAGCAGGGCCAGGTCCGAAGCCGGTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGtt                                                                                                        <  8:121395/149‑1 (MQ=255)
                                               atgcagGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGTGAATCACATCCTGGTCATGTGTCGGGATAGCGCCATGGCGGTGGAGCAGCTACAAC‑AACATGGCCAGACGGTTCTGGTCGAGTCCCAAGGTTa                                                                                                       <  8:27347/147‑1 (MQ=255)
                                                   agggccagggccGAAGCCGTTTCCAGCACCTTCACCCCGCCGAGCACGTTGAGAAACACATCCTGTTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACccg                                                                                                   <  2:240191/149‑1 (MQ=255)
                                                     ggCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACccgcc                                                                                                 >  7:32869/1‑149 (MQ=255)
                                                        cAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCcgcg                                                                                              >  2:110278/1‑149 (MQ=255)
                                                            tCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGtt                                                                                          <  3:97403/149‑1 (MQ=255)
                                                            tCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGtt                                                                                          <  1:110278/149‑1 (MQ=255)
                                                              cGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGCGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTgg                                                                                        >  6:48880/1‑149 (MQ=255)
                                                               gAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGc                                                                                       >  1:302427/1‑149 (MQ=255)
                                                                aaGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGc                                                                                       >  7:171611/1‑148 (MQ=255)
                                                                     gTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCgg                                                                                             <  2:112800/137‑1 (MQ=255)
                                                                     gTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTgggggcggg                                                                                  >  1:112800/1‑142 (MQ=255)
                                                                      tttCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATgg                                                                                >  6:329119/1‑149 (MQ=255)
                                                                        tCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCATCTAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATgg                                                                                <  1:93120/148‑1 (MQ=255)
                                                                          cAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCAcc                                                                                                                                                   >  5:323566/1‑79 (MQ=255)
                                                                          cAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCAcc                                                                                                                                                   <  6:323566/79‑1 (MQ=255)
                                                                          cAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGtt                                                                                                                           >  4:203524/1‑102 (MQ=255)
                                                                          cAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGtt                                                                                                                           <  3:203524/102‑1 (MQ=255)
                                                                               ccTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTAtcgtcg                                                                       <  3:220947/149‑1 (MQ=255)
                                                                                    aCCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAg                                                                  >  2:192726/1‑149 (MQ=255)
                                                                                     ccccGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgc                                                                 <  1:132251/149‑1 (MQ=255)
                                                                                       ccgccgACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgcgc                                                               <  4:178388/149‑1 (MQ=255)
                                                                                          ccgACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTg                                                            <  2:302427/149‑1 (MQ=255)
                                                                                             aCCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCAc                                                         >  7:99731/1‑149 (MQ=255)
                                                                                              ccACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTAtcgtc                                                                        <  1:270174/133‑1 (MQ=255)
                                                                                              ccACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTAtcgtc                                                                        >  2:270174/1‑133 (MQ=255)
                                                                                               cACGTTCAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCt                                                       <  1:192726/149‑1 (MQ=255)
                                                                                                aCGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTc                                                      >  1:168931/1‑149 (MQ=255)
                                                                                                aCGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTc                                                      <  7:39695/149‑1 (MQ=255)
                                                                                                  gTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGCTGGCTTTCGTCGACCAGCGCGTGCACCGCGa                                                    >  6:219242/1‑149 (MQ=255)
                                                                                                        aaaCACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCa                                              >  6:5407/1‑149 (MQ=255)
                                                                                                          acacATCCTGCTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCAtt                                            <  6:268286/149‑1 (MQ=255)
                                                                                                              aTCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAgg                                                                                                          <  6:110212/83‑1 (MQ=255)
                                                                                                              aTCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAgg                                                                                                          >  5:110212/1‑83 (MQ=255)
                                                                                                               tCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCg                                       <  5:5407/149‑1 (MQ=255)
                                                                                                                    gTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTa                                   <  7:197461/148‑1 (MQ=255)
                                                                                                                       aTGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgcgc                                                               <  5:92680/117‑1 (MQ=255)
                                                                                                                       aTGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgcgc                                                               >  6:92680/1‑117 (MQ=255)
                                                                                                                        tGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTc                              >  5:258889/1‑149 (MQ=255)
                                                                                                                           gTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCCCCGCGACCAGCATTGGCCGGGTAGCCTcc                             >  3:65661/1‑147 (MQ=255)
                                                                                                                           gTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCa                           <  8:32869/149‑1 (MQ=255)
                                                                                                                              gggATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGCCCAGCATTGGCCGGGTACCCTCCCAGGt                        >  7:170471/1‑149 (MQ=255)
                                                                                                                              gggATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGt                        >  2:143043/1‑149 (MQ=255)
                                                                                                                                gATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTg                      >  6:144562/1‑149 (MQ=255)
                                                                                                                                gATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTg                      <  8:99731/149‑1 (MQ=255)
                                                                                                                                    ccGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCAt                  <  2:168931/149‑1 (MQ=255)
                                                                                                                                     cGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGCCCAGCATTGGCCGGGTACCCTCCCAGGTTGCCAt                  >  4:131804/1‑148 (MQ=255)
                                                                                                                                      ttgttGGCGGTGCTCCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCCCCCAGGTTGCCATca                >  8:224995/5‑149 (MQ=255)
                                                                                                                                         ttGGCGGTGCTCCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCgg                                      <  6:185751/123‑1 (MQ=255)
                                                                                                                                         ttGGCGGTGCTCCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCgg                                      >  5:185751/2‑124 (MQ=255)
                                                                                                                                         ttGGCGGTGCTCCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcacca             >  6:145230/2‑149 (MQ=255)
                                                                                                                                         aTGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcacca             >  5:217066/1‑149 (MQ=255)
                                                                                                                                          tGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcaccac            <  8:171611/149‑1 (MQ=255)
                                                                                                                                                    gCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGCTACCCGGc  <  5:48880/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAAC‑AACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGCTACCCGGC  >  NC_002947/5269167‑5269463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑