| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,315,981 | A→G | 7.3% | intergenic (‑49/+102) | PP_4677 ← / ← ilvC | CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase/ketol‑acid reductoisomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,315,981 | 0 | A | G | 7.3% | 151.6 / 2.6 | 55 | intergenic (‑49/+102) | PP_4677/ilvC | CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase/ketol‑acid reductoisomerase |
| Reads supporting (aligned to +/- strand): ref base A (27/24); new base G (2/2); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
TTCGGCGTCATGCCCTTCTTCAACGTGCTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGTCGACGATTTTGTTAGCCGAGATCCACGGC > NC_002947/5315840‑5316128 | ttCGGCGTCATGCCCTTCTTCAACGTGCTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGt > 5:224410/1‑148 (MQ=255) cGGCGTCATGCCCTTCTTCAACGTGCTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGtt < 8:274871/147‑1 (MQ=255) cGGCGTCATGCCCTTCTTCAACGTGCTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTc < 4:318563/148‑1 (MQ=255) ggCGTCATGCCCTTCTTCAACGTGCTCAACGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCt < 8:4738/148‑1 (MQ=255) gCGTCATGCCCTTCTTCAACGTGCTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGa > 2:3877/1‑149 (MQ=255) cttcAACGTGCTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCAc < 3:216502/147‑1 (MQ=255) cAACGTGCTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTg < 2:42910/146‑1 (MQ=255) aaCGTGCTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCt > 3:234018/1‑149 (MQ=255) cTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGGCTGCttt < 5:77161/148‑1 (MQ=255) aTCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTtat < 1:3877/147‑1 (MQ=255) gACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCt < 1:299798/140‑1 (MQ=255) gACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCt > 2:299798/1‑140 (MQ=255) aCAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAg > 5:281188/1‑148 (MQ=255) cAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACTGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGaa < 3:81299/149‑1 (MQ=255) gCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCt < 6:52194/110‑1 (MQ=255) gCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCt > 5:52194/1‑110 (MQ=255) ggCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGc < 1:145004/149‑1 (MQ=255) ctgtagtcGTGGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCa < 6:224410/142‑1 (MQ=255) cTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCa < 4:234018/149‑1 (MQ=255) tttCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGCCACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGc > 8:214545/1‑146 (MQ=255) cGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAAc > 3:257228/1‑149 (MQ=255) ggCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACg < 3:50661/149‑1 (MQ=255) ttGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGct > 3:54075/1‑148 (MQ=255) ttGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGcc > 6:42961/1‑149 (MQ=255) gCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCcg < 2:213154/149‑1 (MQ=255) ctctTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCTACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCcgc < 6:163885/149‑1 (MQ=255) gggACGTTCGCTCATGGAGTGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCTTCGGCCGCGttttt < 4:54075/149‑1 (MQ=255) ttCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCtt > 2:94912/1‑149 (MQ=255) ttCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCtt > 8:46470/1‑149 (MQ=255) gCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTc > 1:214693/1‑148 (MQ=255) gCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTc < 4:257228/148‑1 (MQ=255) gCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTGCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTc < 1:179508/148‑1 (MQ=255) tGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACAAGGCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGAt > 1:97397/1‑148 (MQ=255) tGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGAt > 3:117366/1‑146 (MQ=255) gggTACCTTGCAACAGGATGGAAAATGATCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGACAAGAcc > 1:10433/1‑147 (MQ=255) tACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGaaa < 2:279312/104‑1 (MQ=255) tACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGaaa > 1:279312/1‑104 (MQ=255) ttGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCtt > 1:288199/1‑149 (MQ=255) cAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTgg < 6:281188/148‑1 (MQ=255) aGGATGGAAAATGTTCGACACGGGCCCGCGAGACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTg < 3:251621/143‑1 (MQ=255) aGGATGGAAAATGTTCGACACGGGCCCGCGAGACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTg > 4:256028/1‑143 (MQ=255) aGGATGGAAAATGTTCGACACGGGCCCGCGAGACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTg > 4:251621/1‑143 (MQ=255) aGGATGGAAAATGTTCGACACGGGCCCGCGAGACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTg < 3:256028/143‑1 (MQ=255) aTGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGTc > 7:236688/1‑148 (MQ=255) aTGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGAT‑AGACTTAGTTCTTGGTCTTTTcga > 6:928/1‑149 (MQ=255) ggAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGGcgac > 1:117510/1‑149 (MQ=255) gAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGTcgac > 2:152063/1‑148 (MQ=255) aTGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTg < 2:72826/133‑1 (MQ=255) aTGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTg > 1:72826/1‑133 (MQ=255) ttCGACACGGGCCCGCGAAACTGGTTCTGACA‑‑GCGAGCCAATGTTCTGCTTTATACCAGAAGCTGTATGCCAGAACGAAAAAACTCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGTCGACGATTTTGt < 2:117510/149‑1 (MQ=255) ttCGACAAGGGCCCGCGAAACAGGTTCTTCCA‑‑GCTCGACACTGGTCTGCTTTATACCAGAATCTGGATGCCAGAACGAAAAAACGCGGCCGAATCCGCGTTTTTCAACTTTCGATAAGACGTAGATCTTGGTCTTGGCGACGATTTTGt > 2:64013/1‑149 (MQ=255) tCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGTCGACGATTTTGt > 3:306690/1‑148 (MQ=255) gACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGTCGACGATTTTGTTa < 4:306690/148‑1 (MQ=255) cccGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGGTCTTGGTCTTGTCGACGATTTTGTTAGCCGAGAt > 5:87463/1‑148 (MQ=255) gAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGTCGACGATTTTGTTAGCCGAGATCCACgg > 4:124728/1‑149 (MQ=255) aaaCAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGTCGACGATTTTGTTAGCCGAGATCCACGGc < 3:124728/149‑1 (MQ=255) | TTCGGCGTCATGCCCTTCTTCAACGTGCTCATCGACAGGTAGCAGGCTTTCGGCGTCGGAGGGCTTGTTCGGCTCTTCGGGACGTTCGCTCATGGAGGGTACCTTGCAACAGGATGGAAAATGTTCGACACGGGCCCGCGAAACAGGTTCTGACA‑‑GCGAGCCACTGGTCTGCTTTATACCAGAAGCTGGATGCCAGAACGAAAAAACGCGGCCGAAGCCGCGTTTTTCATCTTTCGATAAGACTTAGTTCTTGGTCTTGTCGACGATTTTGTTAGCCGAGATCCACGGC > NC_002947/5315840‑5316128 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |