Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,364,423	G→T	7.8%	P71T (CCG→ACG) ‡	folP ←	dihydropteroate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,364,423	0	G	T	7.8%	281.6 / 10.0	90	P71T (CCG→ACG) ‡	folP	dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base G (42/41);  new base T (2/5);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

GCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTA  >  NC_002947/5364281‑5364571                                                                                                                                                |                                                                                                                                                     gCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGt                                                                                                                                                <  2:29947/149‑1 (MQ=255)  cccccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCCCCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGt                                                                                                                                                <  1:85102/149‑1 (MQ=255)  cccccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTg                                                                                                                                               <  4:299879/149‑1 (MQ=255)    cccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCCCGGGTGCGCGTgca                                                                                                                                             <  4:13808/149‑3 (MQ=255)    cccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCCCGGGTGCGCGTgca                                                                                                                                             <  4:174676/149‑3 (MQ=255)    cccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCCCGGGTGCGCGTgca                                                                                                                                             <  2:181013/149‑3 (MQ=255)     ccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGAc                                                                                                                                            >  5:127036/1‑149 (MQ=255)      cAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCCAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCCCGGGTGCGCGTgcagt                                                                                                                                           <  4:293192/149‑5 (MQ=255)        ggCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCCCGGGTGCGCGTgcagtct                                                                                                                                         <  7:263642/149‑7 (MQ=255)        ggCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGt                                                                                                                                                >  3:316367/1‑142 (MQ=255)        ggCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGt                                                                                                                                                <  4:316367/142‑1 (MQ=255)         gcgcgcGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGc                                                                                                                                        <  3:309058/149‑1 (MQ=255)               ggCTTCACGCATGACAGCAGGCGTGGAGGTATCGTCCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTACACGCGCACCCGGGAGCGTGGACTCGCCACCGa                                                                                                                                  >  3:42945/1‑149 (MQ=255)                  ttCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGatat                                                                                                                               <  2:63023/149‑1 (MQ=255)                     aCGCATGAAACCACCCGTGGAGGTATCGACCGAAATGACCAAGTCGAGGCGACTGTTGATCTCCTCTACAAATAG‑CTACACACGCTCCAGCTCCTCGGTCACCTACACACCACGCGCACCCTGGCGCGTCGAATAGCCACCGATATCg                                                                                                                             >  8:94816/1‑148 (MQ=255)                       gCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGAt                                                                                                                           >  8:248829/1‑148 (MQ=255)                        cATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCa                                                                                                                         <  2:38353/149‑1 (MQ=255)                              agcagGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTg                                                                                                                   >  6:9652/1‑149 (MQ=255)                                 agGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTgcgc                                                                                                                <  5:77011/149‑1 (MQ=255)                                   gCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCcg                                                                                                              >  2:112407/1‑149 (MQ=255)                                     gTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCcggc                                                                                                            >  6:234916/1‑149 (MQ=255)                                      tGGAGGTATCGACCGAAATGACCACATCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCcggcg                                                                                                           <  5:242557/149‑1 (MQ=255)                                             aTCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCa                                                                                                    >  3:259199/1‑149 (MQ=255)                                              tCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCAt                                                                                                   <  8:39492/149‑1 (MQ=255)                                                gACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATc                                                                                                  <  6:180688/148‑1 (MQ=255)                                                gACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCg                                                                                                 >  8:86108/1‑149 (MQ=255)                                                     aaaTGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCATCGTTGCGCCGGCGGCAACCATCGCTTCg                                                                                            <  1:183315/149‑1 (MQ=255)                                                     aaaTGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCg                                                                                            >  1:280967/1‑149 (MQ=255)                                                     aaaTGACCAAGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCg                                                                                            <  2:111814/149‑1 (MQ=255)                                                       aTGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGc                                                                                          >  5:279333/1‑149 (MQ=255)                                                          aCCACGTCTAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGGTCCAGCTCCTCGGTCACCGAGACTGCTCGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGt                                                                                        <  8:106792/148‑1 (MQ=255)                                                          aCCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTg                                                                                       <  6:192604/149‑1 (MQ=255)                                                           ccACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTgg                                                                                      <  6:127036/149‑1 (MQ=255)                                                            cACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGgc                                                                                     <  6:279333/149‑1 (MQ=255)                                                            cACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGgc                                                                                     >  4:265216/1‑149 (MQ=255)                                                            cACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGgc                                                                                     >  1:54818/1‑149 (MQ=255)                                                             aCGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGgcg                                                                                    >  5:92746/1‑149 (MQ=255)                                                                  gAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAgg                                                                                <  4:259199/148‑1 (MQ=255)                                                                    ggCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGc                                                                                          <  7:154057/136‑1 (MQ=255)                                                                    ggCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGc                                                                                          >  8:154057/1‑136 (MQ=255)                                                                    ggCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGcc                                                                             <  4:58056/149‑1 (MQ=255)                                                                          tGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGCTGCAGGGCCtcgtcg                                                                       >  1:276479/1‑149 (MQ=255)                                                                             cagtcgCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCgcgc                                                                    <  8:60446/144‑1 (MQ=255)                                                                              gATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCgcgc                                                                    <  5:97154/148‑1 (MQ=255)                                                                                   ccTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctgact                                                               >  6:129579/1‑148 (MQ=255)                                                                                   ccTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctgact                                                               >  2:92426/1‑148 (MQ=255)                                                                                   ccTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTAGCGctgact                                                               >  4:185909/1‑148 (MQ=255)                                                                                   ccTCGACCATTGG‑CGCCAAACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCCCCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGTCAACCATCGATTGCGCCTGCCGCACGGCCTCGTCGCGctgact                                                               >  8:285908/1‑148 (MQ=255)                                                                                       gACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGAGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGCCTGAAGc                                                          >  3:228158/1‑149 (MQ=255)                                                                                          cATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGAc                                                       <  7:263569/149‑1 (MQ=255)                                                                                          cATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGAc                                                       >  4:70807/1‑149 (MQ=255)                                                                                          cATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGAc                                                       >  3:292739/1‑149 (MQ=255)                                                                                                  gcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATc                                                <  2:280967/149‑1 (MQ=255)                                                                                                    cacaCGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCg                                               >  3:174284/1‑148 (MQ=255)                                                                                                      cacGCTCCAGCTCCTCGGTCACCGAGACTGTACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCg                                               >  1:234204/1‑146 (MQ=255)                                                                                                      cacGCTCCAGCTCCTCGGTCACCGAGACTGTACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCg                                               <  2:234204/146‑1 (MQ=255)                                                                                                       acGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGgag                                            >  2:56868/1‑148 (MQ=255)                                                                                                        cGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGaa                                          <  5:234916/149‑1 (MQ=255)                                                                                                               gCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCg                                   <  1:56868/149‑1 (MQ=255)                                                                                                                ctcctcGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                                 >  8:92796/1‑118 (MQ=255)                                                                                                                ctcctcGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                                 <  7:92796/118‑1 (MQ=255)                                                                                                                ctcctcGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCATTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgg                                  <  4:3703/149‑1 (MQ=255)                                                                                                                 tcctcGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATAGATAAACGTTGCGCCGGCGGCAACCATCGCATCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCggg                                 >  5:227542/1‑149 (MQ=255)                                                                                                                       gTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAATCGACCGCCATCGGAGAAGGAATCGCGGGTGa                            >  5:180220/1‑148 (MQ=255)                                                                                                                       gTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACAGCCATCGGAGAAGGAATCGGGGGTGa                            >  1:45813/1‑148 (MQ=255)                                                                                                                        tCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgggg                                <  7:253702/143‑1 (MQ=255)                                                                                                                        tCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgggg                                >  8:253702/1‑143 (MQ=255)                                                                                                                          aCCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATAtt                        >  1:68212/1‑149 (MQ=255)                                                                                                                          aCCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATAtt                        <  7:45710/149‑1 (MQ=255)                                                                                                                            cGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgg                                  <  4:247206/137‑1 (MQ=255)                                                                                                                            cGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgg                                  >  3:247206/1‑137 (MQ=255)                                                                                                                            cGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATAtt                        >  5:230328/1‑147 (MQ=255)                                                                                                                            cGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTga                      <  2:54818/149‑1 (MQ=255)                                                                                                                            cGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTga                      <  6:92746/149‑1 (MQ=255)                                                                                                                              agaCTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCCGAGAAGGAATCGGGGGTGATATTgaga                    <  3:70807/149‑1 (MQ=255)                                                                                                                               gagtccACGCGCCCGGGTGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTgaga                    >  7:156195/6‑148 (MQ=255)                                                                                                                                aCTACACGCGCACCCGGGTGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGaa                   <  4:42945/148‑1 (MQ=255)                                                                                                                                 cTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGAc                                                       <  3:250427/111‑1 (MQ=255)                                                                                                                                 cTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGAc                                                       >  4:250427/1‑111 (MQ=255)                                                                                                                                  tGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATAc                >  3:208620/1‑149 (MQ=255)                                                                                                                                    cACGCGCCCGGGTGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATAcc               >  7:82750/1‑148 (MQ=255)                                                                                                                                     aCGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGCAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCa             >  7:75863/1‑149 (MQ=255)                                                                                                                                     aCGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCa             >  7:45264/1‑149 (MQ=255)                                                                                                                                      cgcgCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCAt            <  5:51476/149‑1 (MQ=255)                                                                                                                                           aCCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCa             <  6:7984/143‑1 (MQ=255)                                                                                                                                           aCCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCa             >  5:7984/1‑143 (MQ=255)                                                                                                                                            cccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGatat                         <  5:299337/130‑1 (MQ=255)                                                                                                                                            cccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGatat                         >  6:299337/1‑130 (MQ=255)                                                                                                                                             ccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGTCGTGGAGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGAGATTGAGAATACCCATGCAATgg     >  4:229072/1‑149 (MQ=255)                                                                                                                                             ccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATgg     <  4:174284/149‑1 (MQ=255)                                                                                                                                                ggCTCGTTGACTCGACACCTATATCGATCAACGTTGCGCCTGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTa  <  4:228158/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGG‑CGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTA  >  NC_002947/5364281‑5364571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑