Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,382,270	A→T	8.8%	I125F (ATC→TTC)	smpB →	SsrA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,382,270	0	A	T	8.8%	162.4 / 5.9	57	I125F (ATC→TTC)	smpB	SsrA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (27/25);  new base T (2/3);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

CCACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGGGCA  >  NC_002947/5382125‑5382415                                                                                                                                                  |                                                                                                                                                     ccACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCg                                                                                                                                                   >  2:209639/1‑149 (MQ=255) ccACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCg                                                                                                                                                   >  8:256922/1‑149 (MQ=255) ccACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCg                                                                                                                                                   <  6:42647/149‑1 (MQ=255)    cGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATTTCGCAc                                                                                                                                                <  7:304286/149‑1 (MQ=255)      tCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTg                                                                                                                                              <  5:120639/149‑1 (MQ=255)           gCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATTTCGCACttgatc                                                                                                                                          <  3:73647/148‑6 (MQ=255)            ccGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaag                                                                                                                                        >  6:288129/1‑149 (MQ=255)              gACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaaggg                                                                                                                                      <  7:256922/149‑1 (MQ=255)                cccTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaagggc                                                                                                                                     >  2:101047/1‑148 (MQ=255)                           cccGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGCAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTc                                                                                                                         <  5:279274/149‑1 (MQ=255)                             cGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGa                                                                                                                       >  1:282317/1‑149 (MQ=255)                                  gctgctgctGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAgc                                                                                                                 >  1:133223/1‑149 (MQ=255)                                        gctgAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTAGA‑CAAGCGCGaca                                                                                                           >  5:290867/1‑149 (MQ=255)                                           gAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCa                                                                                                        >  1:317325/1‑149 (MQ=255)                                              cAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATg‑‑c                                                                                                   <  5:260055/149‑1 (MQ=255)                                                 gcgcGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATg‑‑cgc                                                                                                 >  2:266194/1‑148 (MQ=255)                                                        cTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGa                                                                                         <  2:282317/149‑1 (MQ=255)                                                           gAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGAtt                                                                                       <  5:4100/148‑1 (MQ=255)                                                                 gTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACcg                                                                                >  1:150876/1‑149 (MQ=255)                                                                    gAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGa                                                                             <  1:209639/149‑1 (MQ=255)                                                                     aaGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGACCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCTAGGGCAATAAGGAATTCGA‑CAATCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAg                                                                            <  7:48938/149‑1 (MQ=255)                                                                      aGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGc                                                                           <  7:290382/149‑1 (MQ=255)                                                                       gCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCt                                                                          >  1:327718/1‑149 (MQ=255)                                                                       gCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCt                                                                          >  4:3143/1‑149 (MQ=255)                                                                        cGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTg                                                                         <  8:85915/149‑1 (MQ=255)                                                                             gTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTCGGCAAGGGCAAGAAGGAATACAAGCAAGCGCGACACATTG‑‑CGCGAACGCGATTCAAACCGCTCGATGCAGc                                                                     >  1:105003/1‑149 (MQ=255)                                                                                 cgcAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCt                                                                <  6:289563/149‑1 (MQ=255)                                                                                  gcAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCtg                                                               >  6:89814/1‑149 (MQ=255)                                                                                    aaaaaGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCtgtg                                                             >  3:122095/1‑149 (MQ=255)                                                                                    aaaaaGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCtgtg                                                             >  6:145784/1‑149 (MQ=255)                                                                                      aaaGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCg                                                           <  2:133223/149‑1 (MQ=255)                                                                                          gCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAAc                                                       <  1:266194/149‑1 (MQ=255)                                                                                             acacCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAg                                                    >  5:208151/1‑149 (MQ=255)                                                                                              cacCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAg                                                    <  5:288129/148‑1 (MQ=255)                                                                                                        gCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAgg                                             >  4:208267/1‑145 (MQ=255)                                                                                                         cTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAagag                                        <  2:327718/149‑1 (MQ=255)                                                                                                          tttGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAagag                                        >  1:227654/1‑148 (MQ=255)                                                                                                          tttGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAagag                                        >  4:222744/1‑148 (MQ=255)                                                                                                            tGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGt                                       <  7:67956/147‑1 (MQ=255)                                                                                                            tGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGt                                       <  2:150876/147‑1 (MQ=255)                                                                                                                    tACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTActct                              <  3:208267/148‑1 (MQ=255)                                                                                                                      cTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATGCCCCCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCtt                             <  1:101047/149‑1 (MQ=255)                                                                                                                      cTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTc                            >  2:36304/1‑148 (MQ=255)                                                                                                                         gAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAAc                         <  4:60405/148‑1 (MQ=255)                                                                                                                         gAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAAc                         <  4:122095/148‑1 (MQ=255)                                                                                                                         gAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAAc                         >  3:60405/1‑148 (MQ=255)                                                                                                                            caagcaCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGt                     >  6:230552/1‑149 (MQ=255)                                                                                                                                caCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCg                 >  5:160049/1‑149 (MQ=255)                                                                                                                                        ccaGTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAg                                              <  4:218604/110‑1 (MQ=255)                                                                                                                                        ccaGTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAg                                              >  3:218604/3‑112 (MQ=255)                                                                                                                                        ccaGTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTc         >  5:316669/3‑149 (MQ=255)                                                                                                                                           gTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTcgc       <  2:303729/148‑1 (MQ=255)                                                                                                                                           gTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTcgc       >  1:303729/1‑148 (MQ=255)                                                                                                                                             gCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCgg     <  3:18733/148‑1 (MQ=255)                                                                                                                                             gCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCgg     >  4:18733/1‑148 (MQ=255)                                                                                                                                              cGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCggg    <  2:317325/148‑1 (MQ=255)                                                                                                                                               gAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGGGCa  >  1:279975/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                     CCACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGA‑CAAGCGCGACACCATG‑‑CGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGGGCA  >  NC_002947/5382125‑5382415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑