Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,418,820	A→C	12.9%	V84G (GTA→GGA) ‡	PP_4758 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,418,820	0	A	C	12.9%	162.7 / 13.3	61	V84G (GTA→GGA) ‡	PP_4758	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (27/26);  new base C (4/4);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACA  >  NC_002947/5418674‑5418967                                                                                                                                                    |                                                                                                                                                    cGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑Ac                                                                                                                                                    <  8:85599/148‑1 (MQ=255) cGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑Ac                                                                                                                                                    <  8:269809/148‑1 (MQ=255)    ccACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCg                                                                                                                                                <  4:132639/149‑1 (MQ=255)      aCCCCGAGCAGAAAACGCAGCACGTAGAACTGGGGTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGGGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTA‑CCTTCGaa                                                                                                                                              <  2:105381/149‑1 (MQ=255)        cccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACa                                                                                                                                            <  4:580/149‑1 (MQ=255)         ccgcccAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCCTGGCCGTGGCCACCATCCCCCAGGTGATCATGATACGGGTCAGCCACACCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTA‑CCTTCGAACat                                                                                                                                           <  8:117355/144‑2 (MQ=255)                        agcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATgaa                                                                                                                            <  8:171920/149‑1 (MQ=255)                                gAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGc                                                                                                                     <  1:19807/148‑1 (MQ=255)                                       gTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCAc                                                                                                              >  7:45546/1‑148 (MQ=255)                                        tttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCACGGCAcc                                                                                                             >  7:117481/1‑148 (MQ=255)                                        tttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGAGGAAGAACAGCCCGGCAcc                                                                                                             >  7:140423/1‑148 (MQ=255)                                             ttCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTA‑CCTTCGAACat                                                                                                                                           <  4:293485/113‑2 (MQ=255)                                             ttCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTA‑CCTTCGAACat                                                                                                                                           >  3:293485/1‑112 (MQ=255)                                                  cacaAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACAGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCAt                                                                                                     <  1:68749/146‑1 (MQ=255)                                                   acaAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGc                                                                                                 >  2:157198/1‑149 (MQ=255)                                                     aaaGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCAACGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCg                                                                                               >  8:21700/1‑149 (MQ=255)                                                       aGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCa                                                                                             <  1:157198/149‑1 (MQ=255)                                                       aGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAACCCATAGGCCGc                                                                                              >  4:8607/1‑148 (MQ=255)                                                          ccATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAgcg                                                                                          >  5:113932/1‑149 (MQ=255)                                                           cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACAGATGAAGAACAGCCCGGCCCCGAAGCCATCGGCCGCAgcgc                                                                                         >  3:197889/1‑149 (MQ=255)                                                                   tGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGccc                                                                                 <  6:113932/149‑1 (MQ=255)                                                                          caGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCTGCAGCGCTGATGCCCAGGt                                                                             <  3:239816/146‑1 (MQ=255)                                                                          caGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCTGCAGCGCTGATGCCCAGGt                                                                             >  4:239816/1‑146 (MQ=255)                                                                                ccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACGGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTc                                                                      >  5:276227/1‑147 (MQ=255)                                                                                    gTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTg                                                                <  7:129266/149‑1 (MQ=255)                                                                                    gTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCAAAGGT‑ACATCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTAGCTTTCCACGTg                                                                >  1:252167/1‑149 (MQ=255)                                                                                     tGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgc                                                               <  3:45587/149‑1 (MQ=255)                                                                                          aTGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcg                                                          <  8:45546/149‑1 (MQ=255)                                                                                            gATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGa                                                        >  3:252978/1‑149 (MQ=255)                                                                                             aTACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGa                                                        <  7:225131/148‑1 (MQ=255)                                                                                             aTACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGAc                                                       >  5:39336/1‑149 (MQ=255)                                                                                             aTACGGGGCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGa                                                        <  3:77304/148‑1 (MQ=255)                                                                                                 gggTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAg                                                   >  6:196159/1‑149 (MQ=255)                                                                                                   gTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGc                                                  >  2:141494/1‑148 (MQ=255)                                                                                                     cAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑AATTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCAAGGTGCGGGCGGACGAAGCCaa                                               >  4:84584/1‑149 (MQ=255)                                                                                                         cacaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGtt                                           <  1:141494/149‑1 (MQ=255)                                                                                                          acaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCCCCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGtt                                           >  7:296556/1‑148 (MQ=255)                                                                                                            aGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGAc                                        >  3:272943/1‑149 (MQ=255)                                                                                                                gggCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTcc                                                                     >  4:98472/1‑116 (MQ=255)                                                                                                                gggCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTcc                                                                     <  3:98472/116‑1 (MQ=255)                                                                                                                 ggCACCGACCTTTTGCAGCAGCATGTTCGAAGGTAACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCgg                                    >  1:212688/1‑149 (MQ=255)                                                                                                                 ggCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCa                                                                    <  3:228735/116‑1 (MQ=255)                                                                                                                 ggCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCa                                                                    >  4:228735/1‑116 (MQ=255)                                                                                                                     ccGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                               <  4:252978/149‑1 (MQ=255)                                                                                                                      cGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAgg                              >  8:218557/1‑149 (MQ=255)                                                                                                                      cGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCAATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAgg                              >  5:164131/1‑149 (MQ=255)                                                                                                                       gACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                             <  1:128473/149‑1 (MQ=255)                                                                                                                             ttGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGtt                         <  3:8607/147‑1 (MQ=255)                                                                                                                              tGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGa                       <  2:211620/148‑1 (MQ=255)                                                                                                                               gcagcagcaTGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGAc                      >  3:257255/1‑148 (MQ=255)                                                                                                                                   cagcaTGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                             <  3:91871/137‑1 (MQ=255)                                                                                                                                   cagcaTGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                             >  4:91871/1‑137 (MQ=255)                                                                                                                                    agcaTGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGaa                <  6:276227/149‑1 (MQ=255)                                                                                                                                        tGTTCGAAGTA‑CCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTa                            <  8:285226/133‑1 (MQ=255)                                                                                                                                        tGTTCGAAGTA‑CCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTa                            >  7:285226/1‑133 (MQ=255)                                                                                                                                        tGTTCGAAGTA‑CCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATTAACat             >  5:253538/1‑148 (MQ=255)                                                                                                                                          ttCGAAGTA‑CCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgat          >  2:229075/1‑149 (MQ=255)                                                                                                                                          ttCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgat          <  7:37433/149‑1 (MQ=255)                                                                                                                                          ttCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgat          >  8:37433/1‑149 (MQ=255)                                                                                                                                          ttCGAAGGT‑AATTCGAACAGCGCGTAACCGATGAAGAACAGCCCGCAACCGAAGCCATAGGCCGCAGCCCTAATGCCCAGGTCGCTTTCCAGGTGCGGCCGGACGAAGCCAATGTTGACGCGGTGGAGGAAGTTGACGATGAACatgat          >  7:184144/1‑149 (MQ=255)                                                                                                                                                  t‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGACCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCTGGTCGCTTTCCATGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACa  <  3:84584/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGT‑ACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACA  >  NC_002947/5418674‑5418967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑