Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,440,011 T→C 8.0% D272G (GAC→GGC)  amn ← AMP nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,440,0110TC 8.0% 166.9 / 5.1 62D272G (GAC→GGC) amnAMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base T (31/26);  new base C (3/2);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01

GTAGTCGCCGATGGTCTGCGACTGCCGCAGGCCGCCGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGCGCGGGTCGTCACGC  >  NC_002947/5439863‑5440157
                                                                                                                                                    |                                                                                                                                                   
gTAGTCGCCGATGGTCTGCGACTGCCGCAGGCCGCCGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGt                                                                                                                                                     <  1:17609/149‑1 (MQ=255)
      gCCGATGGTCTGCGACTGCCGCAGGCCGCCGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTcggcgg                                                                                                                                               >  2:114041/1‑149 (MQ=255)
          aTGGTCTGCGACTGCCGCCGGCCGCCGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGGCGGTGATGTTCTTGGCGCTGGATGCGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGCCGACGGCGAt                                                                                                                                           <  4:206148/149‑1 (MQ=255)
           tGGTCTGCGACTGCCGCAGGCCGCCGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATc                                                                                                                                          <  6:77828/149‑1 (MQ=255)
           tGGTCTGCGACTGCCGCAGGCCGCCGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGCCGACGGCGAtg                                                                                                                                          <  7:8086/149‑2 (MQ=255)
              tCTGCGACTGCCGCAGGCCGCCGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGGCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAgg                                                                                                                                       <  2:216123/149‑1 (MQ=255)
                             ggCCGCCGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCAACGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATc                                                                                                                        <  4:193296/149‑1 (MQ=255)
                              gccgccGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCt                                                                                                                       <  4:44598/149‑1 (MQ=255)
                                          aCCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGcc                                                                                                           <  6:382/149‑1 (MQ=255)
                                            cGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGc                                                                                                            >  7:122959/1‑146 (MQ=255)
                                               tcatcaGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGAcc                                                                                                      <  2:19090/149‑1 (MQ=255)
                                                 atcaGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCAc                                                                                                    >  3:264625/1‑149 (MQ=255)
                                                 atcaGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCAc                                                                                                    >  6:222147/1‑149 (MQ=255)
                                                   cagccagcAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGc                                                                                                  >  1:224630/1‑149 (MQ=255)
                                                    agccagcAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCt                                                                                                 >  6:19216/1‑149 (MQ=255)
                                                          ccaTGCGGGGGCACCCAGGCCAGGTGGGTGGGGTATTTCTTGGCCGTGGATTGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGTTAGCGGTGCCAGACCACGCTGG‑ccac                                                                                          <  3:249414/147‑1 (MQ=255)
                                                          cAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑ccac                                                                                          >  1:312229/1‑149 (MQ=255)
                                                               cGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑ccacca                                                                                        >  1:197512/1‑146 (MQ=255)
                                                               cGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑ccacca                                                                                        <  2:197512/146‑1 (MQ=255)
                                                                gggCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGc                                                                                    <  3:56021/149‑1 (MQ=255)
                                                                  gcgcAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACGACGCTGG‑CCACCAGGGCCt                                                                                  >  8:226091/1‑149 (MQ=255)
                                                                   cgcAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTg                                                                                 <  8:278820/149‑1 (MQ=255)
                                                                    gcagcaCGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTgg                                                                                <  8:122959/149‑1 (MQ=255)
                                                                    gcagcaCGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGAGCAGGTGGTAGGCCGGCAGCTGGTAGCGGTGCCAGACCCCGCTGG‑CCACCAGGGCCTgg                                                                                >  4:177269/1‑149 (MQ=255)
                                                                      agcaCGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGt                                                                              >  7:13947/1‑149 (MQ=255)
                                                                      agcaCGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGt                                                                              >  1:38284/1‑149 (MQ=255)
                                                                             ccAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCa                                                                       <  5:19216/149‑1 (MQ=255)
                                                                               aGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATg                                                                     <  4:264625/149‑1 (MQ=255)
                                                                                   ggtCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTcc                                                                 <  8:13947/149‑1 (MQ=255)
                                                                                                 ttGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGc                                                                                    >  1:155191/1‑116 (MQ=255)
                                                                                                 ttGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGc                                                                                    <  2:155191/116‑1 (MQ=255)
                                                                                                   ggCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATc                                                  <  3:92171/148‑1 (MQ=255)
                                                                                                   ggCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATc                                                  <  1:114041/148‑1 (MQ=255)
                                                                                                     cGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCacg                                               <  2:224630/149‑1 (MQ=255)
                                                                                                         ggATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGt                                           >  4:99743/1‑149 (MQ=255)
                                                                                                          gATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGtt                                          >  8:46164/1‑149 (MQ=255)
                                                                                                                cccACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCgg                                    <  3:99743/149‑1 (MQ=255)
                                                                                                                cccACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCgg                                    <  5:270964/149‑1 (MQ=255)
                                                                                                                cccACACCAATGTTGACCAAGGTAACGCAATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCgg                                    >  8:266411/1‑149 (MQ=255)
                                                                                                                 ccACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCtttt                                                       >  4:87253/1‑129 (MQ=255)
                                                                                                                 ccACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCtttt                                                       <  3:87253/129‑1 (MQ=255)
                                                                                                                  cacaCCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCgg                                    >  4:210850/1‑147 (MQ=255)
                                                                                                                    cacCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGcc                                                                        >  5:175475/1‑109 (MQ=255)
                                                                                                                    cacCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGcc                                                                        <  6:175475/109‑1 (MQ=255)
                                                                                                                     acCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGgcagca                               >  7:14271/1‑149 (MQ=255)
                                                                                                                       cAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCAc                              >  3:185694/1‑148 (MQ=255)
                                                                                                                        aaTGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATAAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCATGGCCTGGGTTTCGACATGGTCCATGCCCGTTTCGATCACGACGTTGCCCGGCAGCACCa                            >  5:9069/1‑149 (MQ=255)
                                                                                                                          tGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGTCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCAta                          <  5:222147/149‑1 (MQ=255)
                                                                                                                              gACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCAtacgta                      >  1:170327/1‑149 (MQ=255)
                                                                                                                              gACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCAtacgt                       <  2:312229/148‑1 (MQ=255)
                                                                                                                                 cAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCAtacgtacg                    >  1:39186/1‑148 (MQ=255)
                                                                                                                                      tAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCtttt                                                       >  3:317419/1‑108 (MQ=255)
                                                                                                                                      tAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCtttt                                                       <  4:317419/108‑1 (MQ=255)
                                                                                                                                       aaCGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGcgcg             >  4:169404/1‑149 (MQ=255)
                                                                                                                                       aaCGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGcgcg             >  3:170830/1‑149 (MQ=255)
                                                                                                                                        aCGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGCGCgg            >  4:110273/1‑149 (MQ=255)
                                                                                                                                         cctgaTCGCCGCCGACGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCGCCATACGTACGAAGCGCggg           >  5:122051/5‑149 (MQ=255)
                                                                                                                                          ctgaTCGCCGCCGACGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGCGCGGgt          >  1:188191/4‑149 (MQ=255)
                                                                                                                                           tgaTCGCCGCCGACGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGGACCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGCGCGGgt          >  1:97379/3‑149 (MQ=255)
                                                                                                                                            cATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGCGCGGgtcg        >  5:237320/1‑149 (MQ=255)
                                                                                                                                             aTCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGCGCGGgtcgt       >  2:162158/1‑149 (MQ=255)
                                                                                                                                             aTCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGCGCGGgtcgt       >  4:36986/1‑149 (MQ=255)
                                                                                                                                                  cGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGCGCGGGTCGTCACGc  <  7:266411/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTAGTCGCCGATGGTCTGCGACTGCCGCAGGCCGCCGCAGTGACCGATCATCAGCCAGCAATGCGGGCGCAGCACGGCCAGGTGGTCGGTGATGTTCTTGGCGTTGGATGGGCCCACACCAATGTTGACCAAGGTAACGCCATCGCCGTCGGCGGCGATCAGGTGGTAGGCCGGCATCTGGTAGCGGTGCCAGACCACGCTGG‑CCACCAGGGCCTGGGTTTCGCCATGGTCCATGCCCTTTTCGATCACGACGTTGCCCGGCAGCACCATACGTACGAAGCGCGGGTCGTCACGC  >  NC_002947/5439863‑5440157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑