Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,470,378 G→A 7.8% V509V (GTC→GTT)  mrdA‑II ← transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,470,3780GA 7.8% 148.0 / 2.0 51V509V (GTC→GTT) mrdA‑IItranspeptidase
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base A (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

TTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATCAGTGCAGTGGCCTGGGCCAGTT  >  NC_002947/5470238‑5470523
                                                                                                                                            |                                                                                                                                                 
ttgCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGc                                                                                                                                           <  4:134513/149‑1 (MQ=255)
ttgCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCGCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTgg                                                                                                                                            <  5:177013/148‑1 (MQ=255)
        gTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCg                                                                                                                                    <  4:309156/148‑1 (MQ=255)
           cTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGAc                                                                                                                                 <  1:279187/148‑1 (MQ=255)
            ttCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGcc                                                                                                                                          <  7:120469/138‑1 (MQ=255)
            ttCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGcc                                                                                                                                          >  8:120469/1‑138 (MQ=255)
             tctcGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGa                                                                                                                                  <  7:24436/145‑1 (MQ=255)
             tctcGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGa                                                                                                                                  >  8:24436/1‑145 (MQ=255)
              ctcGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGcc                                                                                                                                          <  7:278351/136‑1 (MQ=255)
              ctcGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGcc                                                                                                                                          >  8:278351/1‑136 (MQ=255)
              ctcGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTAACCTTGGCCCAGTCGGACTTg                                                                                                                              >  3:12688/1‑148 (MQ=255)
              ctcGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTAACCTTGGCCCAGTCGGACTTGt                                                                                                                             >  3:8295/1‑149 (MQ=255)
                 gCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGt                                                                                                                             <  1:229231/146‑1 (MQ=255)
                       cTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTa                                                                                                                    <  2:89457/149‑1 (MQ=255)
                        ttGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGTCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCTGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTAc                                                                                                                   <  6:291342/149‑1 (MQ=255)
                           aTCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGAt                                                                                                                <  6:188274/149‑1 (MQ=255)
                            tCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATg                                                                                                               >  8:170891/1‑149 (MQ=255)
                                             gTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTgtc                                                                                              <  8:88230/149‑1 (MQ=255)
                                             gTACCGCGCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCGTCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGGCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTgtc                                                                                              <  2:261130/149‑1 (MQ=255)
                                                  gCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTa                                                                                         >  7:106580/1‑149 (MQ=255)
                                                   ctctTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTAc                                                                                        >  5:137541/1‑149 (MQ=255)
                                                   ctctTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTAc                                                                                        >  1:219232/1‑149 (MQ=255)
                                                   ctctTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTAc                                                                                        >  6:168585/1‑149 (MQ=255)
                                                    tctTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTAcc                                                                                       >  4:73371/1‑149 (MQ=255)
                                                    tctTGCCGGCAAGGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTAcc                                                                                       <  7:275100/149‑1 (MQ=255)
                                                        gCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTAACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACcggc                                                                                    <  4:8295/148‑1 (MQ=255)
                                                        gCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTAACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACcggc                                                                                    <  4:12688/148‑1 (MQ=255)
                                                                tGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCt                                                                           >  3:96830/1‑149 (MQ=255)
                                                                 gCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCtt                                                                          >  7:296167/1‑149 (MQ=255)
                                                                    gTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGa                                                                       <  5:168585/149‑1 (MQ=255)
                                                                        tGGGCTCCGGCAGCTGCCTTGCGCGCGGTACGGCGGGCGTTGTGCATCACCTGTTCCATGGCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGGAGTACGATGTCTTCAATGGCGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGt                                                                   >  6:299382/1‑149 (MQ=255)
                                                                          ggCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTc                                                                  <  7:170891/148‑1 (MQ=255)
                                                                          ggCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCt                                                                 <  4:96830/149‑1 (MQ=255)
                                                                               cGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGcc                                                            <  2:219232/149‑1 (MQ=255)
                                                                                        ccTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTgcggg                                                   >  6:93222/1‑149 (MQ=255)
                                                                                         cTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTgcgggc                                                  >  2:12500/1‑149 (MQ=255)
                                                                                                gcgGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCca                                           >  7:238573/1‑149 (MQ=255)
                                                                                                      ccGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCacacac                                      <  8:106580/148‑1 (MQ=255)
                                                                                                                  tgCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGa                          <  3:73371/148‑1 (MQ=255)
                                                                                                                   gCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGAt                         >  6:63491/1‑148 (MQ=255)
                                                                                                                         ccTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGGCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATcagtg                    >  1:102341/1‑147 (MQ=255)
                                                                                                                           tGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATcagtgcagt                >  6:276899/1‑149 (MQ=255)
                                                                                                                             ttCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATcagtgcagt                <  5:31082/147‑1 (MQ=255)
                                                                                                                               ccatgccatgGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATCAGTGCAGTGGcc            >  5:251890/1‑149 (MQ=255)
                                                                                                                                    ccatgGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGt                                               <  7:62486/109‑1 (MQ=255)
                                                                                                                                    ccatgGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGt                                               >  8:62486/1‑109 (MQ=255)
                                                                                                                                    ccatgGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATCAGTGCAGTGGCCTggg        <  6:137541/148‑1 (MQ=255)
                                                                                                                                      atgGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATCAGTGCAGTGGCCTGGGCCa     <  1:12500/149‑1 (MQ=255)
                                                                                                                                         ggTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATCAGTGCAGTGGCCTGGGCCAGtt  <  6:251890/149‑1 (MQ=255)
                                                                                                                                         ggTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATCAGTGCAGTGGCCTGGGCCAGtt  >  5:184263/1‑149 (MQ=255)
                                                                                                                                         ggTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATCAGTGCAGTGGCCTGGGCCAGtt  >  2:131520/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTCGTCTACCGGCGGCAAGCCTTCGATGGTCTTGGCCAGGTGCGGGCGGTTCCACACACCTTTGTTGGCGATCAGTGCAGTGGCCTGGGCCAGTT  >  NC_002947/5470238‑5470523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑