| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,485,162 | T→C | 18.9% | intergenic (+68/‑79) | purH → / → purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,485,162 | 0 | T | C | 18.9% | 66.6 / 10.9 | 38 | intergenic (+68/‑79) | purH/purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base T (12/18); new base C (3/4); total (15/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01 | |||||||||||
TGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCA > NC_002947/5485019‑5485309 | tGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCAccg > 7:163428/1‑149 (MQ=255) aaGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACcgccgc < 8:163428/148‑1 (MQ=255) gTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCAtt > 5:148830/1‑149 (MQ=255) aTCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGGCCGCGCATACGAAGGTTGCATCACCCCCGCATTctc > 8:167259/1‑147 (MQ=255) aTCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTcgc > 1:72998/1‑149 (MQ=37) tCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTcgcg > 7:259962/1‑149 (MQ=37) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCTGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCgg < 8:259962/148‑1 (MQ=25) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCggg < 2:72998/149‑1 (MQ=25) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCggg < 6:148830/149‑1 (MQ=255) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg < 6:66676/148‑1 (MQ=255) ctgctgCCGACGAGGCCGGCATCTCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCGGGc < 4:29180/149‑1 (MQ=25) gacgaGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGCATAAGATGGTGGATTCACCGCCGCATTAGCGGGCCAGCCCGc > 7:72773/1‑149 (MQ=255) cGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAAATCCTGTGGGAGCGGGCCTGGCCGCGAATAACATGGTGGGTTAAACGCCCCAATAGCGGGCAACCCCGCTACcacaa > 8:174040/1‑148 (MQ=255) tGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCATTCCCGCCAAAAAGAAGGTGGATTAACCCCCGCAATAGCCCGCAACCACGCTACCCCACTGTTAGGCCCCAGCCACGGGa > 4:225540/1‑149 (MQ=255) aTGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCGTGCCCGCGGATAAGATGGGGGGTTGACCGCCGCATTCGCCGGCAAGCCCGCGCCCACAGGGTTCGGCGCAAGCCACGGGATGAc > 6:296637/1‑149 (MQ=255) gcccaaCTTCCGCCACAAATTACGCGGATCCCGCCGCATGCGGACACCCTGGGGCTGCGGGCGGGCCCGCTAATACCATGGTCGATCCCCCCCCGCATGCGGGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACtt < 8:189955/144‑1 (MQ=255) cgcCACTTCCGCCACTAATTACTCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg < 2:213332/103‑1 (MQ=255) cgcCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg > 1:213332/1‑103 (MQ=255) ccGCCACTAATTACGCGTATCCCGAGGCAAGTCTCGATCCTGGGGGAGCGGGCTTGCCCGCGAATACGATGGGGGATTCACCGCCGCATTCCCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAg < 5:135027/148‑1 (MQ=38) cGCCACTAATTCCGCGGATCCCGAGGCACGGCGAGAGCCTGTGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCaa < 1:209992/124‑1 (MQ=37) gCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCACGCGAATAAGATGGTGGGTTCACCGCCGCCTTAGCGGGCAAGCCCCCGCCCACACTGGTCGGCGCAAGCCCCCGGATCACGTGAATGGAgggt > 4:239799/1‑147 (MQ=255) cTAATTCCGCGGATCCCGAGGCAAGGCGAGATCCTGTGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGa < 1:207298/149‑1 (MQ=255) tAATTCCGCGTATCCCGTGGCATGGCGAGACCCTGTGGTAGGGGGCTGGCCCGCTAATACTATGGTGTATTCCCCGCCCCATGCGGGGCAAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGAc < 4:271087/149‑1 (MQ=255) cgcgGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGaaa < 3:225540/149‑1 (MQ=255) tCCCGTGGCAAGTCGAGATCGTGTGGGAGCGGGCTTGCCCGCTAATACTATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGtttt < 8:72773/148‑1 (MQ=255) cctaGGAAAGTCGAGATCGTGTGGGAGCGGGCTTCCCGGCTAATACGATGGTGTATTCACCGCCGAATTCGCGGGAAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGa < 7:167259/145‑1 (MQ=255) ccGAGGCAAGTCGAGATCCTGTGGGAGCCGGCTTGCCCGCTAATACGATGGGGTATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGa < 6:302412/148‑1 (MQ=255) ggCAAGTCGATATCCTGGGGCAGCGGGCTTCCCGGACAATACTATGTTGAATTCACCGCCCAATTCGCGGTAATGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGatcatc < 3:51429/149‑1 (MQ=255) gcgCGGGGTTGCCGGCTAATACGTTGGTGGATTCACCGCCGCATGCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGa < 3:129838/144‑1 (MQ=255) ggCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTg < 7:19575/118‑1 (MQ=255) ggCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTg > 8:19575/1‑118 (MQ=255) ggCTTGCCCGCCAATACGATGGTGCATTCACCGCCCCATTCCCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGccc < 3:22785/149‑1 (MQ=255) ttGCCCGCGAATACGATGGTGGATTCACAGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctgg > 7:176724/1‑149 (MQ=255) cccGCTAATACGATGGTGGATTCACCGCCGCTTTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcct < 5:327752/149‑1 (MQ=255) cgcgAATGCGGCGGTGAATCCACCATCGTATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg > 3:304334/1‑148 (MQ=37) cgcgAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg > 8:237297/1‑148 (MQ=255) ccGATGGGGCATGCCCCGCCGCATCCGCGGCCACCCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCg < 3:45024/148‑1 (MQ=255) tcgATTCACCGCCGCATGCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGa < 5:117092/147‑1 (MQ=255) ttCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCa > 2:275171/1‑149 (MQ=255) | TGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCA > NC_002947/5485019‑5485309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |