Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 421,165 T→C 5.6% H928R (CAC→CGC)  PP_0348 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947421,1650TC 5.6% 259.4 / 4.1 90H928R (CAC→CGC) PP_0348hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (41/43);  new base C (2/3);  total (43/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

GCACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAGT  >  NC_002947/421017‑421309
                                                                                                                                                    |                                                                                                                                                  
gCACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGt                                                                                                                                                    >  6:75899/1‑149 (MQ=255)
  aCGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTgg                                                                                                                                                  <  6:285679/149‑1 (MQ=255)
    gCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑Gc                                                                                                                                               <  5:327323/149‑1 (MQ=255)
    gCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑Gc                                                                                                                                               >  1:53027/1‑149 (MQ=255)
         ccAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGC‑CCACTGACAGCGGAGCcggcg                                                                                                                                          <  6:97408/149‑1 (MQ=255)
          cagctcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcggcgg                                                                                                                                         <  8:270621/149‑1 (MQ=255)
            gctcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGt                                                                                                                                                    >  2:74054/1‑137 (MQ=255)
            gctcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCggtg                                                                                                                                       <  1:284496/149‑1 (MQ=255)
              tcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCggtggt                                                                                                                                     >  2:151270/1‑149 (MQ=255)
                agctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTg                                                                                                                                   <  2:273660/149‑1 (MQ=255)
                agctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGACCGCCGGC‑CCACTGACAGCGGAGCcggcggcctc                                                                                                                                     <  7:245852/147‑5 (MQ=38)
                       ccccGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTGGCCAAAACTGGTCGACTCAGGCCGCCGGCTCCGCTGTCTGGGG‑GCCGGCGGTGGTTGATCTGGc                                                                                                                            <  3:316158/149‑1 (MQ=255)
                        cccGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCATTGG‑GCCGGCGGTGGTTGATCTgg                                                                                                                             >  4:81932/1‑147 (MQ=255)
                        cccGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTgg                                                                                                                             >  5:300082/1‑147 (MQ=255)
                         ccGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCTGTGGTTTATCTGGCAg                                                                                                                          <  8:99584/149‑1 (MQ=255)
                              cTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCa                                                                                                                     >  4:94258/1‑149 (MQ=255)
                               tATGCCCGTGACCGGGCACGGCTGCTACGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAg                                                                                                                    >  2:282372/1‑149 (MQ=255)
                                 tGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑c                                                                                                                  <  5:75899/148‑1 (MQ=255)
                                 tGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCACCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑c                                                                                                                  <  8:248944/148‑1 (MQ=255)
                                    ccGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGAGTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAgt                                                                                                                         <  4:232566/139‑1 (MQ=255)
                                    ccGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAgt                                                                                                                         >  3:232566/1‑139 (MQ=255)
                                     cGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGt                                                                                                              >  6:195357/1‑148 (MQ=255)
                                     cGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCAGCTCCGCTGTCAGTGG‑GCCGGCCGTGGTTGATCTGGCAGTGTCAG‑CCAGt                                                                                                              >  3:76312/1‑148 (MQ=255)
                                         aCCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCActg                                                                                                         <  2:281679/149‑1 (MQ=255)
                                          ccGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCActgc                                                                                                        >  8:118554/1‑149 (MQ=255)
                                           cGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGGCAGTGG‑GCCGGCGGTGGTTTATCTGGCAGTGTCAG‑CCAGTCActgct                                                                                                       >  6:115333/1‑149 (MQ=255)
                                               cACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTgcgc                                                                                                   <  7:118554/149‑1 (MQ=255)
                                                aCGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑ACAGTCACTGATGCGcc                                                                                                  >  6:256201/1‑149 (MQ=255)
                                                  ggCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCcct                                                                                                >  1:221954/1‑149 (MQ=255)
                                                    ctgctgCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCcctgc                                                                                              <  2:221954/149‑1 (MQ=255)
                                                      gctgcGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCcctgcct                                                                                            >  6:74644/1‑149 (MQ=255)
                                                       ctgcGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCcctgcctg                                                                                           <  2:133131/149‑1 (MQ=255)
                                                         gcgcAACTCCCCCACTGGCAGTGCCTTCAATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑CCCGGCGGTGGTTGATCTCGCAGTCTCAG‑CCAGTCACTGCTGCCCcctcaatggc                                                                                         >  8:252887/1‑142 (MQ=255)
                                                          cgcAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTgg                                                                                          <  1:282372/147‑1 (MQ=255)
                                                             aaCTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGTTGTTGCCAATACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAAc                                                                                     <  5:323803/149‑1 (MQ=255)
                                                               cTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAAcgc                                                                                   <  5:74644/149‑1 (MQ=255)
                                                               cTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAAcgc                                                                                   <  8:107918/149‑1 (MQ=255)
                                                               cTGGCACACTGGCAGTGCCTTGCAGCAGCGGATGGACCGGCTGTTGCCAAACCTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGGCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAAcgc                                                                                   <  5:115333/149‑1 (MQ=255)
                                                                      aCTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAg                                                                            >  7:183987/1‑149 (MQ=255)
                                                                       cTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGc                                                                           >  5:137624/1‑149 (MQ=255)
                                                                          gCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGATCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCAc                                                                        >  1:231759/1‑149 (MQ=255)
                                                                            aGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTg                                                                      <  1:151270/149‑1 (MQ=255)
                                                                             gTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTgg                                                                     <  2:82697/149‑1 (MQ=255)
                                                                                     cATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTc                                                             <  3:94258/149‑1 (MQ=255)
                                                                                       tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCcctgcctg                                                                                           >  2:57606/1‑117 (MQ=255)
                                                                                       tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCcctgcctg                                                                                           <  1:57606/117‑1 (MQ=255)
                                                                                       tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCAc                                                                        >  4:139269/1‑136 (MQ=255)
                                                                                       tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCAc                                                                        <  3:139269/136‑1 (MQ=255)
                                                                                       tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGt                                                           >  3:36715/1‑149 (MQ=255)
                                                                                       tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCATATCAGCCACTGGCCGTTTTCGt                                                           >  5:78933/1‑149 (MQ=255)
                                                                                        cAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCATATCAGCCACTGGCCGTTTTCGt                                                           <  6:78933/148‑1 (MQ=255)
                                                                                         aGCTGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTgg                                                         <  2:53027/149‑1 (MQ=255)
                                                                                         aGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCAGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTgg                                                         <  4:76312/149‑1 (MQ=255)
                                                                                             gCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCGCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCgca                                                     <  6:300082/149‑1 (MQ=255)
                                                                                               tGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCgcagc                                                   >  3:245101/1‑149 (MQ=255)
                                                                                                 gACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCgcagca                                                  <  5:195357/148‑1 (MQ=255)
                                                                                                  aCCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCAcc                                                >  2:64902/1‑149 (MQ=255)
                                                                                                    cGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTg                                                          <  6:218423/137‑1 (MQ=255)
                                                                                                    cGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTg                                                          >  5:218423/1‑137 (MQ=255)
                                                                                                          ttGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCAc                                          <  1:323482/147‑1 (MQ=255)
                                                                                                            gCCAAAACTGGTCGACTGAGGCCGCCGCCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCACTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGca                                      >  7:37934/1‑149 (MQ=255)
                                                                                                             ccAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGca                                      <  8:284827/148‑1 (MQ=255)
                                                                                                                aaaCTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcaccac                                  <  3:77696/149‑1 (MQ=255)
                                                                                                                 aaCTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGACTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCcccccc                                 >  2:212964/1‑149 (MQ=255)
                                                                                                                     ggTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCgg                             >  2:320860/1‑149 (MQ=255)
                                                                                                                      gTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GACGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACAGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCCTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCCGc                            >  7:187752/1‑149 (MQ=255)
                                                                                                                       tCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCg                           >  8:108691/1‑149 (MQ=255)
                                                                                                                         gACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGtt                         >  2:142900/1‑149 (MQ=255)
                                                                                                                         gACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGtt                         >  5:222801/1‑149 (MQ=255)
                                                                                                                          aCTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑GCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATGAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTg                        <  3:135067/149‑1 (MQ=255)
                                                                                                                          aCTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTg                        <  7:252887/149‑1 (MQ=255)
                                                                                                                            tGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGcc                      >  1:322047/1‑148 (MQ=255)
                                                                                                                             gAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTg                     >  6:255312/1‑149 (MQ=255)
                                                                                                                             accaccgccgGC‑CCACTGACAGCGGAGCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTg                     >  4:130639/5‑149 (MQ=255)
                                                                                                                             accaccgccgGC‑CCACTGACAGCGGAGCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTg                     <  3:130639/145‑1 (MQ=255)
                                                                                                                              ccaccgccgGC‑CCACTGACAGCGGAGCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTgg                    >  2:42797/4‑149 (MQ=255)
                                                                                                                              aGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTgg                    <  2:303786/149‑1 (MQ=255)
                                                                                                                              aGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTgg                    >  3:305039/1‑149 (MQ=255)
                                                                                                                              aGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTgg                    >  3:310041/1‑149 (MQ=255)
                                                                                                                               ggCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGAGCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTgg                    >  1:84916/1‑149 (MQ=255)
                                                                                                                                 ccgccgGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCaa                 <  5:88287/149‑1 (MQ=255)
                                                                                                                                 ccgcagGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGAACCGCCCACGGCACCACCCCGGCGTTGCTGGCac                 >  6:106351/1‑148 (MQ=255)
                                                                                                                                   gccgGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCCAGCAACg               >  6:91630/1‑149 (MQ=255)
                                                                                                                                     cgGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGc             <  4:245101/149‑1 (MQ=255)
                                                                                                                                      ggCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCCAGCAACgccg            <  2:322047/149‑4 (MQ=255)
                                                                                                                                       gCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCCAGCAACgccgg           <  5:91630/149‑5 (MQ=255)
                                                                                                                                        cTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAt          <  6:137624/149‑1 (MQ=255)
                                                                                                                                           cGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAtctc       >  5:149130/1‑149 (MQ=255)
                                                                                                                                           cGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAtctc       <  4:36715/149‑1 (MQ=255)
                                                                                                                                            gCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAtctc       >  1:253282/1‑148 (MQ=255)
                                                                                                                                              tGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCaa    <  6:222801/149‑1 (MQ=255)
                                                                                                                                                tCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAGt  <  1:142900/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAG‑CCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAGT  >  NC_002947/421017‑421309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑