Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,538,577 G→T 11.7% intergenic (+67/+54) PP_4871 → / ← PP_4872 lysine decarboxylase family protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,538,5770GT11.7% 116.8 / 4.4 42intergenic (+67/+54)PP_4871/PP_4872lysine decarboxylase family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (18/19);  new base T (2/3);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCCTGCTGGTTACGCGCA  >  NC_002947/5538429‑5538724
                                                                                                                                                    |                                                                                                                                                   
gcagcCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATgg                                                                                                                                                     <  7:180222/149‑1 (MQ=255)
         cGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGc                                                                                                                                            >  3:21828/1‑149 (MQ=255)
            gctgctGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTACGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCtt                                                                                                                                         <  3:173053/149‑1 (MQ=255)
            gctgcgGGAAGGGATGGACAGCTTGGTGGCGCCGGGCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCtt                                                                                                                                         <  4:34057/149‑1 (MQ=37)
             ctgctgGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGc                                                                                                                                            <  8:143708/145‑1 (MQ=38)
             ctgctgGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGc                                                                                                                                            >  7:143708/1‑145 (MQ=38)
               gctgGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTtgtg                                                                                                                                      >  3:315781/1‑149 (MQ=255)
                ctgGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTgtgt                                                                                                                                     <  6:170911/149‑1 (MQ=255)
                   gAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTcgc                                                                                                                                  <  8:99021/149‑1 (MQ=255)
                   gAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTc                                                                                                                                    <  1:163047/147‑1 (MQ=255)
                             aCAGTTTTGTGGCGGGGTTCGGCCTTATGTGGGTCTACATGCTGCTTGACTTATGGTTAGAGTTTTGGGACGGTTTCGGGGTCTTTTCGCGACACAAGGCCGCTCCCACAAAGGAAATGGGGTAGGGGCCTTGTGTCGCGATGGGCTGc                                                                                                                        <  4:21828/149‑1 (MQ=255)
                                         cgcCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATggg                                                                                                                                                    >  4:20779/1‑109 (MQ=255)
                                         cgcCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATggg                                                                                                                                                    <  3:20779/109‑1 (MQ=255)
                                                   gcCTAAGTGGGTCGACAAGCAGCCTGACTTACGCGTAGACTTTGGGGACCGCTTCGCGGTCCTTTCGCGACACACGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATc                                                                                                  <  4:215978/149‑1 (MQ=38)
                                                   gcCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTcgc                                                                                                                                  <  2:73516/117‑1 (MQ=21)
                                                   gcCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTcgc                                                                                                                                  >  1:73516/1‑117 (MQ=21)
                                                         gTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTaa                                                                                            <  3:83544/149‑1 (MQ=255)
                                                             gTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACAGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAACGCAATGGGGGACCGGCCTTGTGTCCCGATGGGCTGCAAAGCAGCACCAAAAATCGAACAGTTAAcgag                                                                                        >  4:256400/1‑147 (MQ=38)
                                                              tCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAAcgcg                                                                                        >  2:201329/1‑148 (MQ=255)
                                                                   aaGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGa                                                                                  <  4:315781/149‑1 (MQ=255)
                                                                    agcagcCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCACCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAAAAGTCGATCAGTTAACGCGGAATGa                                                                                  >  1:146929/1‑148 (MQ=255)
                                                                         ccTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCt                                                                            >  4:183800/1‑149 (MQ=255)
                                                                             actgaAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCCAAGCAGCCCCAATAGTCGATCAGTTTACGCGGCATGACAGGCTGAcg                                                                        >  7:106407/1‑149 (MQ=255)
                                                                                 aaGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGc                                                                    <  1:201329/149‑1 (MQ=255)
                                                                                   gCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGc                                                                    <  8:186533/147‑1 (MQ=255)
                                                                                   gCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGc                                                                    >  7:186533/1‑147 (MQ=255)
                                                                                    cttcgaCTTTTGGGACCGCTTCGCGGTCCTTTCGCCACACAAGGCCGCTCCCACAAAGGCAATGGGGGCGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGcttc                                                                 <  5:280541/145‑1 (MQ=255)
                                                                                      gagaCTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGAAGGGCTGCAAAGCAGCCCCAAAAGTCGATCAGTTAAACCGGAATGACAGGCTGACGCGGcttctt                                                               >  3:72176/1‑149 (MQ=255)
                                                                                       agtcTTTTGGGTCGTATTCGTGGTCGTTTCGGGAATCATGGCCGCTCCCACAATGGGAATGGGTGAGGGGCGTTGTGTCTCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGcttctt                                                               <  3:256400/145‑1 (MQ=255)
                                                                                       agaCTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACCGGCTGACGCGGCTTCttg                                                              >  7:138545/1‑149 (MQ=255)
                                                                                             ttGCGACCGCTTCGCGGTCCTTGCGCGACACAAGCCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGcc                                                         <  8:127535/148‑1 (MQ=255)
                                                                                                ggACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGCATGACAGGCTGACGCGGCTGCTTGTTGCCCTTg                                                     >  5:93567/1‑149 (MQ=255)
                                                                                                          gcgGTCCTTTCGCGACACAAGGCCGCTCCCACAACGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCttgttg                                                           <  6:252269/133‑1 (MQ=255)
                                                                                                          gcgGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCttgttg                                                           >  5:252269/1‑133 (MQ=255)
                                                                                                                 tttCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAAt                                                                                    <  4:261686/101‑1 (MQ=255)
                                                                                                                 tttCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAAt                                                                                    >  3:261686/1‑101 (MQ=255)
                                                                                                                  ttCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCAcc                                                <  1:227480/136‑1 (MQ=255)
                                                                                                                  ttCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCAcc                                                >  2:227480/1‑136 (MQ=255)
                                                                                                                      cgACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCATGTTGCCCTTGCCACCCTTGCTCGCCTCTTTgc                               >  3:25336/1‑149 (MQ=255)
                                                                                                                          acacGGCCGCTCCCACAATGGAATGGGGGGTGCGGCGTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTc                           <  2:146929/149‑1 (MQ=39)
                                                                                                                                 cGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTgg                      >  1:326850/1‑147 (MQ=255)
                                                                                                                                  gCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAg                   >  1:154585/1‑149 (MQ=255)
                                                                                                                                         caAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCctgctg            <  2:326850/149‑1 (MQ=255)
                                                                                                                                              gCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGc                     <  4:247180/135‑1 (MQ=255)
                                                                                                                                              gCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGc                     >  3:247180/1‑135 (MQ=255)
                                                                                                                                                    ggggAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCCTGCTGGTTACGCGCa  >  8:279076/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAGCCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCCTGCTGGTTACGCGCA  >  NC_002947/5538429‑5538724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑