Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,624,806	C→G	6.3%	G319R (GGC→CGC)	PP_4943 ←	glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,624,806	0	C	G	6.3%	245.1 / 4.4	76	G319R (GGC→CGC)	PP_4943	glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (37/34);  new base G (2/3);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGAACTCAAAGCGAACACGTCGAA  >  NC_002947/5624660‑5624950                                                                                                                                                   |                                                                                                                                                  tgGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGGTCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGcc                                                                                                                                                   <  4:277170/147‑1 (MQ=255)   ggAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑c                                                                                                                                              <  1:65587/149‑1 (MQ=255)      gTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAACCGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGGGCGACGCCGCGCAG‑Ctgg                                                                                                                                           <  5:67981/149‑1 (MQ=255)      gTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGGGCGACGCCGCGCAG‑Ctgg                                                                                                                                           <  5:72591/149‑1 (MQ=255)       ttGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑Cggg                                                                                                                                           >  6:21147/1‑148 (MQ=255)          ccAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGTTCCCGCTGGGCGACGCCGCGCAG‑Ctggcc                                                                                                                                         >  5:230152/1‑145 (MQ=255)           cAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGTTCCCGCTGGGCGACGCCGCGCAG‑Ctggcccag                                                                                                                                      <  6:230152/149‑6 (MQ=38)              aaGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCAc                                                                                                                                   >  7:99099/1‑149 (MQ=255)                   cTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCaa                                                                                                                              <  7:158384/149‑1 (MQ=255)                          aCCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTc                                                                                                                       <  2:105431/149‑1 (MQ=255)                               cTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCa                                                                                                                  <  6:149395/149‑1 (MQ=255)                                tGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCAc                                                                                                                 >  5:131982/1‑149 (MQ=255)                                  aTCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACtt                                                                                                               >  3:252895/1‑149 (MQ=255)                                   tCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTc                                                                                                              >  1:196881/1‑149 (MQ=255)                                    cAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTcg                                                                                                             >  3:281854/1‑149 (MQ=255)                                    cAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCCTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGACGCGCAG‑CGGGAACCGCGCGCCAACGCCCACGACCACTTcg                                                                                                             >  8:128376/1‑149 (MQ=255)                                     agagAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTcgc                                                                                                            >  6:26437/1‑149 (MQ=255)                                      gagaAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTcgcg                                                                                                           >  5:1295/1‑149 (MQ=255)                                      gagaAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTcgcg                                                                                                           >  4:239334/1‑149 (MQ=255)                                      gagaAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTcgcg                                                                                                           <  5:275137/149‑1 (MQ=255)                                       agaAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCgg                                                                                                          <  1:788/149‑1 (MQ=255)                                              cTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGc                                                                                                   >  6:320719/1‑149 (MQ=255)                                              cTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGc                                                                                                   <  4:281854/149‑1 (MQ=255)                                                  cccAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCcaca                                                                                               <  4:228705/149‑1 (MQ=255)                                                   ccAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCCGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAg                                                                                              >  2:243998/1‑149 (MQ=255)                                                   ccAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGCCCACTTCGCGGGCACCGCCACAg                                                                                              >  8:296373/1‑149 (MQ=255)                                                   ccAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAg                                                                                              >  5:86228/1‑149 (MQ=255)                                                   ccAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAg                                                                                              <  4:252895/149‑1 (MQ=255)                                                     aGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGc                                                                                            >  8:48631/1‑149 (MQ=255)                                                        cgcTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGt                                                                                         <  3:239334/149‑1 (MQ=255)                                                         gcTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAACACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTg                                                                                        <  8:78328/149‑1 (MQ=255)                                                           tGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGc                                                                                      >  3:125856/1‑149 (MQ=255)                                                             cagcaTGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGc                                                                                      <  3:94025/147‑1 (MQ=255)                                                              agcaTTTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCaa                                                                                   <  7:128376/149‑1 (MQ=255)                                                                caTGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAaca                                                                                 >  4:27498/1‑149 (MQ=255)                                                                     gCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAacaccgg                                                                             >  6:239669/1‑148 (MQ=255)                                                                      ccTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAacaccggca                                                                           >  8:304094/1‑149 (MQ=255)                                                                        tGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGc                                                                                            >  1:141908/1‑130 (MQ=255)                                                                        tGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGc                                                                                            <  2:141908/130‑1 (MQ=255)                                                                           cacaGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAacaccggcacaccg                                                                      <  5:320719/149‑1 (MQ=255)                                                                            acaGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGTCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAacaccggcacaccgg                                                                     >  7:208156/1‑149 (MQ=255)                                                                              aGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAacaccggcacaccgg                                                                     <  5:4302/147‑1 (MQ=255)                                                                              aGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAacaccggcacaccgg                                                                     >  6:4302/1‑147 (MQ=255)                                                                                gCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAAc                                                                 >  6:242639/1‑149 (MQ=255)                                                                                     gcgcTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGcatccatc                                                             <  5:239669/148‑1 (MQ=255)                                                                                           cccAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTc                                                        <  5:316633/147‑1 (MQ=255)                                                                                           cccAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGa                                                      <  6:1295/149‑1 (MQ=255)                                                                                            ccAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAC‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAGCCATCGCTTCGAg                                                     >  7:282215/1‑149 (MQ=255)                                                                                             cAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGc                                                    <  5:26437/149‑1 (MQ=255)                                                                                               gCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAg                                                  <  6:131982/149‑1 (MQ=255)                                                                                                cGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAg                                                  >  2:107409/1‑148 (MQ=255)                                                                                                 gTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGa                                                <  3:27498/149‑1 (MQ=255)                                                                                                 gTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGa                                                <  1:107409/149‑1 (MQ=255)                                                                                                      gTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATg                                           >  7:298497/1‑149 (MQ=255)                                                                                                       tACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGc                                          <  5:242639/149‑1 (MQ=255)                                                                                                           gCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGaa                                      <  2:196881/149‑1 (MQ=255)                                                                                                            ccATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAAc                                     >  5:181174/1‑149 (MQ=255)                                                                                                              aTGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACGCCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACgg                                   >  8:70701/1‑149 (MQ=255)                                                                                                               tgtgCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGc                                  <  3:105612/149‑1 (MQ=255)                                                                                                                 tgctgcAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTc                                <  6:86228/149‑1 (MQ=255)                                                                                                                    tgcAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGt                              >  3:225510/1‑148 (MQ=255)                                                                                                                      cAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTg                             <  4:125856/147‑1 (MQ=255)                                                                                                                         gCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCg                         >  4:274559/1‑148 (MQ=255)                                                                                                                         gCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCg                         <  3:274559/148‑1 (MQ=255)                                                                                                                          cccTGGGCCAGCTGCGCGGCGTCGCCCAGCCGGGACCAGCCCGCCAACGCCCTCGCCCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGCCCATGCCGAACGGCGCGTGATCg                         >  1:29506/1‑148 (MQ=255)                                                                                                                          cccTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCAGGCCGAACGGCTCGGGATCgg                        >  8:235826/1‑148 (MQ=255)                                                                                                                           ccTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACTCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGaa                      <  8:99099/149‑1 (MQ=255)                                                                                                                           ccTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGaa                      <  8:298497/149‑1 (MQ=255)                                                                                                                               ggCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCACCGCCCTCGCCCACTTCGCGGGCACCGCCCCAGGCCGTGGCCAACACCGGCACCCCGGCAACCATCGCTTCGAGCCGGACCATGCCGAACGGCTCGTGATCGGAACTc                   >  3:170589/1‑148 (MQ=255)                                                                                                                               ggCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGAACTCa                  <  4:29210/149‑1 (MQ=255)                                                                                                                                 ccAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGAACTCa                  >  7:10907/1‑147 (MQ=255)                                                                                                                                 ccAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCAGAACTCa                  >  3:246435/1‑147 (MQ=255)                                                                                                                                  cAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGAACTCAAAg               <  7:48631/149‑1 (MQ=255)                                                                                                                                   aGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGACAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGAACTCAAAGc              >  8:56226/1‑149 (MQ=255)                                                                                                                                      tGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGAACTCAAAGCGaa           >  6:141118/1‑149 (MQ=255)                                                                                                                                           ggcgTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGAACTCAAAGCGAACACGt      <  4:225510/149‑1 (MQ=255)                                                                                                                                               tCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGAACTCAAAGCGAACACGTCGaa  >  3:296881/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGCGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAG‑CGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTCGAGCAGGACCATGCCGAACGGCTCGTGATCGGAACTCAAAGCGAACACGTCGAA  >  NC_002947/5624660‑5624950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑