Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,658,129 C→A 6.8% L62M (CTG→ATG)  sahR → methionine metabolism transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,658,1290CA 6.8% 188.1 / 3.6 59L62M (CTG→ATG) sahRmethionine metabolism transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (28/27);  new base A (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01

GAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGCCCGATGTGC  >  NC_002947/5657982‑5658276
                                                                                                                                                   |                                                                                                                                                   
gAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCt                                                                                                                                                    <  8:19766/149‑1 (MQ=255)
     aTTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCt                                                                                                                                                >  1:222491/1‑148 (MQ=255)
      ttGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCt                                                                                                                                                >  6:206818/1‑147 (MQ=255)
      ttGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCt                                                                                                                                                >  6:258457/1‑147 (MQ=255)
        ggCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAg                                                                                                                                             <  7:143529/148‑1 (MQ=255)
        ggCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTC‑GGCCTGAGCCAGCACCTTGAGGTGATGGCTCatg                                                                                                                                            <  6:313571/148‑3 (MQ=38)
         gCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAgg                                                                                                                                            <  8:51856/148‑1 (MQ=255)
         gCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTC‑GGCCTGAGCCAGCACCTTGAGGTGATGGCTCATGcc                                                                                                                                          <  6:149456/149‑1 (MQ=38)
            gcgcTGTGCAAAGCGAGTGGCTATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCATGGTGCTGGCTCAGGCCGa                                                                                                                                        <  8:104285/146‑1 (MQ=255)
                    cAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGGTCAGGCCGACCTCGTgg                                                                                                                                <  2:24971/149‑1 (MQ=255)
                    cAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTg                                                                                                                                 <  1:310141/148‑1 (MQ=255)
                         ccAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTCGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACGACGTGGCAAcc                                                                                                                           >  6:193531/1‑149 (MQ=255)
                                     cgctgcgcCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAgg                                                                                                                <  5:177322/148‑1 (MQ=255)
                                      gctgcgcCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGc                                                                                                               >  5:114730/1‑148 (MQ=255)
                                               gAACGTTTTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCAtct                                                                                                     >  4:318209/1‑149 (MQ=255)
                                                aaCGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCAtctt                                                                                                    <  7:236128/149‑1 (MQ=255)
                                                aaCGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCAtctt                                                                                                    <  6:114730/149‑1 (MQ=255)
                                                         cgcgcCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTAccgcc                                                                                            <  3:194534/148‑1 (MQ=255)
                                                                  gCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGtgccctgcc                                                                                  >  8:106695/1‑149 (MQ=255)
                                                                    cAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCaa                                                                                                                             <  1:239832/104‑1 (MQ=255)
                                                                    cAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCaa                                                                                                                             >  2:239832/1‑104 (MQ=255)
                                                                     aaTGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGAGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGa                                                                               >  3:216890/1‑149 (MQ=255)
                                                                       tGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTTGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATg                                                                             <  6:23474/149‑1 (MQ=255)
                                                                             gTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTgc                                                                       >  2:276172/1‑149 (MQ=255)
                                                                                cGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGTGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTgcgc                                                                     <  6:42797/148‑1 (MQ=255)
                                                                                  gCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTg                                                                  <  5:89577/149‑1 (MQ=255)
                                                                                     tGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGt                                                               >  4:88038/1‑149 (MQ=255)
                                                                                             cTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCt                                                       <  2:282299/149‑1 (MQ=255)
                                                                                               ggCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTg                                                      >  2:176303/1‑148 (MQ=255)
                                                                                                gcgcAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCa                                                    >  6:38650/1‑149 (MQ=255)
                                                                                                  gcAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCAcg                                                  >  2:103597/1‑149 (MQ=255)
                                                                                                  gcAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCAcg                                                  >  2:122073/1‑149 (MQ=255)
                                                                                                         ttCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCgct                                           >  1:123280/1‑149 (MQ=255)
                                                                                                         ttCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCgct                                           >  6:243309/1‑149 (MQ=255)
                                                                                                                tCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaa                                    <  7:106695/149‑1 (MQ=255)
                                                                                                                 cGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATg                                                                             <  8:139138/107‑1 (MQ=255)
                                                                                                                 cGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATg                                                                             >  7:139138/1‑107 (MQ=255)
                                                                                                                   gCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCACCGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaag                                   >  5:232023/1‑147 (MQ=255)
                                                                                                                   gCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaagaa                                 <  5:243309/149‑1 (MQ=255)
                                                                                                                   gCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaagaa                                 <  1:122073/149‑1 (MQ=255)
                                                                                                                   gCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaag                                   >  8:83502/1‑147 (MQ=255)
                                                                                                                   gCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaag                                   >  6:300422/1‑147 (MQ=255)
                                                                                                                   gCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaag                                   >  4:15918/1‑147 (MQ=255)
                                                                                                                   gCCAGTCGGGCAGGAGCCATCACCTCAAGGGGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaagaa                                 <  2:214751/149‑1 (MQ=255)
                                                                                                                     cAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGt                               <  7:83502/149‑1 (MQ=255)
                                                                                                                      aGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTc                              <  5:258457/149‑1 (MQ=255)
                                                                                                                        tCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTcga                            <  3:88038/149‑1 (MQ=255)
                                                                                                                        tCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTcga                            <  5:38650/149‑1 (MQ=255)
                                                                                                                                   ccAGCACCTTGAGGTGATGGCTCATGCCGACCTCGTGGCACCCCGCCGCGAAGGCACCGGCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCGGGCTGGGCGGCTGCACGCGGCGCGGCTCGATGAAGTCGACGAGCTGGccc                 >  8:178361/1‑149 (MQ=255)
                                                                                                                                   ccAGCACCTTGAGGTGATGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGccc                 >  1:297355/1‑149 (MQ=255)
                                                                                                                                     aTCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTgg                                                                 >  2:272119/1‑99 (MQ=255)
                                                                                                                                     aTCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTgg                                                                 <  1:272119/99‑1 (MQ=255)
                                                                                                                                     aTCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTa               >  4:176952/1‑149 (MQ=255)
                                                                                                                                      tCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTAc              >  3:134907/1‑149 (MQ=255)
                                                                                                                                          cTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGcc          >  4:148038/1‑149 (MQ=255)
                                                                                                                                          cTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTCGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGcc          >  8:3365/1‑149 (MQ=255)
                                                                                                                                             aaGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGCCCg        <  6:232023/148‑1 (MQ=255)
                                                                                                                                             aaGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGCCCGa       <  2:222491/149‑1 (MQ=255)
                                                                                                                                               ggTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGCCCGAtg     >  4:169130/1‑149 (MQ=255)
                                                                                                                                                   cTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGCCCGAtgtg   >  5:145694/1‑147 (MQ=255)
                                                                                                                                                   cTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGCCCGAtgtg   >  5:289680/1‑147 (MQ=255)
                                                                                                                                                   cTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGCCCGATGTGc  <  3:15918/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTACCGCCCGATGTGC  >  NC_002947/5657982‑5658276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑