Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,709,378	G→C	7.1%	R1132R (CGG→CGC) ‡	PP_5009 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,709,378	0	G	C	7.1%	258.9 / 7.5	83	R1132R (CGG→CGC) ‡	PP_5009	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (42/34);  new base C (3/3);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CGGTGCAGCCATCGAAAACGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCATTGAACGCAGGACG  >  NC_002947/5709231‑5709522                                                                                                                                                    |                                                                                                                                                 cGGTGCAGCCATCTAAAACGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGATACTGCCGAGCAGATACAGCAATACCTCGATTATCGCTAACgg                                                                                                                                                  <  8:14567/148‑1 (MQ=255)       aGCCATCGAAAACGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCcgc                                                                                                                                            >  8:134926/1‑148 (MQ=255)         ccATCGAAAACGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGAt                                                                                                                                         >  3:224778/1‑149 (MQ=255)         ccATCGAAAACGAAATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCACGCGTTCAAGAGACTTCCGAGAAGATACAGCAATACCTCGATTATCGCGAACTGGTCCGCGAt                                                                                                                                         >  4:231242/1‑149 (MQ=255)          cATCGAAAACGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGAtt                                                                                                                                        <  4:55135/149‑1 (MQ=255)            tCGAAAACGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTcc                                                                                                                                      <  4:113531/149‑1 (MQ=255)             cGAAAACGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTc                                                                                                                                       >  7:246660/1‑147 (MQ=255)               aaaaCGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGGACCCGTTCGCGAtaatcga                                                                                                                                   <  3:253575/149‑7 (MQ=255)               aaaaCGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTccagc                                                                                                                                   >  4:35163/1‑149 (MQ=255)                 aaCGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTccagcca                                                                                                                                 <  2:48825/149‑1 (MQ=255)                 aaCGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTccagc                                                                                                                                   >  6:287004/1‑147 (MQ=255)                  aCGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTccagccag                                                                                                                                >  3:6948/1‑149 (MQ=255)                    gACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAgccaggc                                                                                                                              >  5:65142/1‑149 (MQ=255)                      cATGCTCCTGTGCCGCCGCTTGCTCGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGCGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATAGCGAACCGGTCCGCGATTCCAgccaggcca                                                                                                                            >  2:92166/1‑149 (MQ=255)                         gctgctGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACgg                                                                                                                                                  <  7:261166/124‑1 (MQ=255)                         gctgctGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACgg                                                                                                                                                  >  8:261166/1‑124 (MQ=255)                          ctgctgTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAgccg                                                                                                                        >  4:227902/1‑149 (MQ=255)                            gctgTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGAGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAgccgcc                                                                                                                      >  1:68185/1‑149 (MQ=255)                                  ccgccgCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGc                                                                                                                >  4:303250/1‑149 (MQ=255)                                  ccgccgCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGc                                                                                                                >  1:125427/1‑149 (MQ=255)                                   cgccgcTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCt                                                                                                               >  8:258370/1‑149 (MQ=255)                                     ccgcTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTg                                                                                                             <  2:68185/149‑1 (MQ=255)                                       gcTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTgtc                                                                                                           <  6:64298/149‑1 (MQ=255)                                        cTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCt                                                                                                                  >  7:30068/1‑141 (MQ=255)                                        cTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCt                                                                                                                  <  8:30068/141‑1 (MQ=255)                                        cTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTgtcg                                                                                                          <  7:31137/149‑1 (MQ=255)                                         ttGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTgtcgt                                                                                                         <  4:6948/149‑1 (MQ=255)                                         ttGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGAGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTgtcgt                                                                                                         >  8:135920/1‑149 (MQ=255)                                           gCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCTCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTgtcgt                                                                                                         <  8:246660/147‑1 (MQ=255)                                           gCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTgtcgt                                                                                                         >  6:1019/1‑147 (MQ=255)                                           gCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTgtcgt                                                                                                         >  6:36209/1‑147 (MQ=255)                                            cTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCgtca                                                                                                       <  1:21486/148‑1 (MQ=255)                                             tGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCgtcagt                                                                                                     >  5:206802/1‑149 (MQ=255)                                                   aGGTCGACCACCAGGCGCTGAGCGCACAGTACATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCgg                                                                                               <  3:35163/149‑1 (MQ=255)                                                     gTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTgtcgt                                                                                                         <  1:18547/137‑1 (MQ=255)                                                     gTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTgtcgt                                                                                                         >  2:18547/1‑137 (MQ=255)                                                            accaGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTggatcgg                                                                                      <  7:134926/149‑1 (MQ=255)                                                             ccaGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTTCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTggatcgga                                                                                     <  2:125427/149‑1 (MQ=255)                                                             ccaGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCgtca                                                                                                       <  6:130789/131‑1 (MQ=255)                                                             ccaGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCgtca                                                                                                       >  5:130789/1‑131 (MQ=255)                                                                         cgcACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGaaa                                                                         <  3:26851/149‑1 (MQ=255)                                                                            acaGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAacac                                                                      >  2:34130/1‑149 (MQ=255)                                                                            acaGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAacac                                                                      >  2:38849/1‑149 (MQ=255)                                                                            acaGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAacac                                                                      <  3:303250/149‑1 (MQ=255)                                                                              aGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTg                                                                    <  5:1019/149‑1 (MQ=255)                                                                                   aTCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGa                                                               >  2:221061/1‑149 (MQ=255)                                                                                                 cgTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGAtt                                                 <  1:254761/149‑1 (MQ=255)                                                                                                     ccAGAGACTGCCGAGCAGATACAGCAATCCCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTc                                              <  5:287004/148‑1 (MQ=255)                                                                                                     ccAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGTCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAg                                                                 <  1:260060/129‑1 (MQ=255)                                                                                                     ccAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAg                                                                 >  2:260060/1‑129 (MQ=255)                                                                                                      cAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTcc                                             <  5:36209/148‑1 (MQ=255)                                                                                                      cAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTcc                                             >  1:313749/1‑148 (MQ=255)                                                                                                      cAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTcc                                             <  6:206802/148‑1 (MQ=255)                                                                                                      cAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTc                                              <  5:302357/147‑1 (MQ=255)                                                                                                      cAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTc                                              >  6:302357/1‑147 (MQ=255)                                                                                                          gaCTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATa                                        <  7:258370/149‑1 (MQ=255)                                                                                                            atGCCGAGCATATACATCAATACCTCGATTATCGCGAACGTGTCCGCGATTCCAGCCAGGACATCCGCCGCCTGATTGTCGTCAGTCAGCGGCTGGATCTTATCCTGGCTGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATaaa                                      <  3:231242/148‑1 (MQ=255)                                                                                                               ccGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGcc                                   <  1:92166/149‑1 (MQ=255)                                                                                                               ccGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGc                                    >  3:46743/1‑148 (MQ=255)                                                                                                                   gCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACg                                >  8:84247/1‑148 (MQ=255)                                                                                                                      gATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAAc                            >  5:81079/1‑149 (MQ=255)                                                                                                                       aTACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGaaa                             >  5:5950/1‑147 (MQ=255)                                                                                                                       aTACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGaaa                             <  6:5950/147‑1 (MQ=255)                                                                                                                        tACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTa                          <  2:264526/149‑1 (MQ=255)                                                                                                                         aCAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAg                         >  8:56055/1‑149 (MQ=255)                                                                                                                         aCAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAg                         >  8:51677/1‑149 (MQ=255)                                                                                                                          cAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACGCTGCAGGCCCCCAAGATCGATTTTCCCAATAACGCCACGAAGCTAGc                        >  6:306713/1‑149 (MQ=255)                                                                                                                          cAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGc                        <  1:221061/149‑1 (MQ=255)                                                                                                                           aGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAgccgc                                                                                                                       <  8:243519/53‑1 (MQ=255)                                                                                                                               aTACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGa                   >  3:22772/1‑149 (MQ=255)                                                                                                                                  ccTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGGTCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCa                >  4:275070/1‑149 (MQ=255)                                                                                                                                   cacGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGACAGCCCCCGCATGCTTCTCGTAAGTCACCGGCTGGATCGGATCCTGGCGGAAACACTGAAGGACCCCAAGAACGATATTCCCAATAAATCCAACAAAATATCCAAGAACAt               >  4:38491/3‑149 (MQ=255)                                                                                                                                   cTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCAt               <  4:22772/149‑1 (MQ=255)                                                                                                                                   cTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCAt               >  4:209074/1‑149 (MQ=255)                                                                                                                                    tCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAg                    >  7:287937/1‑143 (MQ=255)                                                                                                                                    tCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAg                    <  8:287937/143‑1 (MQ=255)                                                                                                                                      gATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCAtt              <  7:84247/147‑1 (MQ=255)                                                                                                                                      gATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCAtt              <  6:65142/147‑1 (MQ=255)                                                                                                                                       ggaaTCGCGGACCCGTTCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCaa                     >  7:6098/4‑139 (MQ=255)                                                                                                                                       ggaaTCGCGGACCCGTTCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCaa                     >  5:149139/4‑139 (MQ=255)                                                                                                                                       ggaaTCGCGGACCCGTTCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCaa                     <  6:149139/136‑1 (MQ=255)                                                                                                                                       ggaaTCGCGGACCCGTTCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCaa                     <  8:6098/136‑1 (MQ=255)                                                                                                                                           tCGCGGACCCGTTCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAAATAGCCAAGACCATTGAACg         >  7:108056/1‑147 (MQ=255)                                                                                                                                           tCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCATTGAACg         >  8:205846/1‑147 (MQ=255)                                                                                                                                               gAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTATCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCATTGAACGCAGGAc   >  1:61718/1‑149 (MQ=255)                                                                                                                                                 aCGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGTTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCATTGAACGCAGGACg  >  3:173464/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CGGTGCAGCCATCGAAAACGACATGCTGCTGTGCCGCCGCTTGCTGGAGCAGGTCGACCACCAGGCGCTGAGCGCACAGTCCATCGGGCTGCCCCGCGTTCCAGAGACTGCCGAGCAGATACAGCAATACCTCGATTATCGCGAACGGGTCCGCGATTCCAGCCAGGCCAGCCGCCGCCTGCTTGTCGTCAGTCAGCGGCTGGATCGGATCCTGGCGGAAACACTGCAGGACCCCAAGATCGATTTTCCCAATAAAGCCACGAAACTAGCCAAGACCATTGAACGCAGGACG  >  NC_002947/5709231‑5709522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑