Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,711,864	T→C	6.3%	F235L (TTC→CTC)	ubiE →	ubiquinone/menaquinone biosynthesis C‑methyltransferase UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,711,864	0	T	C	6.3%	177.8 / 2.7	63	F235L (TTC→CTC)	ubiE	ubiquinone/menaquinone biosynthesis C‑methyltransferase UbiE
Reads supporting (aligned to +/- strand):  ref base T (31/28);  new base C (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

AAGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACGGCCCTGCCGCGG  >  NC_002947/5711717‑5712012                                                                                                                                                    |                                                                                                                                                      aaGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCtt                                                                                                                                                      <  6:137702/149‑1 (MQ=255)    gCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGCCCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGa                                                                                                                                                   >  5:85799/1‑149 (MQ=255)     ccTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGAc                                                                                                                                                  <  4:79464/149‑1 (MQ=255)        acgacgCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACcgc                                                                                                                                               >  1:129874/1‑149 (MQ=255)         cgacgCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACcgc                                                                                                                                               <  6:122833/148‑1 (MQ=255)          gacgCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAAGCCGGCCTCGAccatca                                                                                                                                             <  4:25346/149‑5 (MQ=255)           acgCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTc                                                                                                                                            <  4:277462/149‑1 (MQ=255)           acgCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAAGCCGGCCTCGAccatcat                                                                                                                                            <  4:194811/149‑6 (MQ=255)            cgCCTACTCGTTCGCCTTCATGCCGGTGGCTGGCAAGCTGATCACCTATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAACCCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTct                                                                                                                                           <  6:309057/149‑2 (MQ=255)             gCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCAc                                                                                                                                          <  3:128138/149‑1 (MQ=255)               cTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTc                                                                                                                                                     >  8:270844/1‑136 (MQ=255)               cTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTc                                                                                                                                                     <  7:270844/136‑1 (MQ=255)                   tCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACca                                                                                                                                    <  3:125160/149‑1 (MQ=255)                     gTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAACCCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGCCCGCGTCACCTAcccc                                                                                                                                   >  6:55002/1‑146 (MQ=255)                         gcactCATGCCGCTGGCTGGCAAGCTGATCACCACTCACGCCGAGACCTACCGTTACCTCCCCGAGTCGATCCGCATGCACCCGGACCAGGAACCCCTGATGAGCAGGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAt                                                                                                                              <  6:215201/145‑1 (MQ=255)                                tGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGc                                                                                                                      >  7:67748/1‑149 (MQ=255)                                  ccGCTGGATGGCAAGCTGATCACCAATGACGCAGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATACACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCg                                                                                                                     <  1:49788/148‑1 (MQ=255)                                          tggcAAGCTGATCACCAATGACGCAGAGAGCTACCGTTACCTCGCCGAGTCGCTCCGCATGCACCCGGACCAGGAACCCCTGAAGAGCATCATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGAGGCATCCTTg                                                                                                            >  6:22096/1‑149 (MQ=255)                                             cAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCg                                                                                                         >  6:316378/1‑149 (MQ=255)                                               aGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTg                                                                                                       <  2:56696/149‑1 (MQ=255)                                                gCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGc                                                                                                      >  7:133993/1‑149 (MQ=255)                                                       aCCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCgggg                                                                                               >  5:78101/1‑149 (MQ=255)                                                       aCCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCggg                                                                                                <  6:297909/148‑1 (MQ=255)                                                        ccAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCTGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCgggg                                                                                               >  2:287939/1‑148 (MQ=255)                                                        ccAAAGACTCCAATAGCTACCCATACCTCGCCGACTCCAACCGCAAGCACCCGGACAAGGAAACCCTCAATACCAACATGCTCAAGGCCAGCTTCCACAGCGTCACCTACCACAACAT‑GACCAGCGGCATAGATTCCGAACACCgcgc                                                                                               >  4:3092/1‑145 (MQ=255)                                                               cGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTTAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGc                                                                                       <  8:211053/149‑1 (MQ=255)                                                                gCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGcc                                                                                      >  2:93683/1‑149 (MQ=255)                                                                  cGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCt                                                                                    >  8:34536/1‑149 (MQ=255)                                                                           ccGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCt                                                                           <  6:78101/149‑1 (MQ=255)                                                                                   tCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGTAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCgg                                                                                                 <  1:104405/119‑1 (MQ=255)                                                                                   tCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCgg                                                                                                 >  2:104405/1‑119 (MQ=255)                                                                                      ccGAGTCGATCCGCATGCACCCGGACCAGTAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACAACAACATCTCCCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGgctg                                                                 <  1:93683/149‑1 (MQ=255)                                                                                             gATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAg                                                         >  1:191389/1‑149 (MQ=255)                                                                                               tCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCg                                                       >  2:174449/1‑149 (MQ=255)                                                                                                    aTGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGaa                                                  <  7:34536/149‑1 (MQ=255)                                                                                                     tGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGaa                                                  >  8:263054/1‑148 (MQ=255)                                                                                                      gCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACa                                                >  7:260320/1‑149 (MQ=255)                                                                                                        aCCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATg                                              >  5:317246/1‑149 (MQ=255)                                                                                                        aCCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATg                                              >  2:158285/1‑149 (MQ=255)                                                                                                        aCCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATg                                              >  2:179121/1‑149 (MQ=255)                                                                                                                      aCCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTc                                <  1:174449/149‑1 (MQ=255)                                                                                                                        ccTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCt                              >  2:268090/1‑149 (MQ=255)                                                                                                                            aaGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTgc                            <  6:85799/147‑1 (MQ=255)                                                                                                                               accATGAGGGGCGAGGCCGGCTGCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTg                       <  8:99510/147‑1 (MQ=255)                                                                                                                                 cttgatgGGCGAGTGCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGAGCAAGCCCTGATGTGGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGa                     <  5:22096/147‑1 (MQ=255)                                                                                                                                 cATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGTGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGa                     >  4:261075/1‑149 (MQ=255)                                                                                                                                 cATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGa                     >  7:203984/1‑149 (MQ=255)                                                                                                                                      tgGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTgc                            >  5:287709/1‑137 (MQ=255)                                                                                                                                      tgGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTgc                            <  6:287709/137‑1 (MQ=255)                                                                                                                                      cggTCGAAGCCGGCCTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCa                >  7:279601/2‑149 (MQ=255)                                                                                                                                       ggTCGAAGCCGGCCTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCAc               >  6:90261/1‑149 (MQ=255)                                                                                                                                         tCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACgg             >  6:47818/1‑149 (MQ=255)                                                                                                                                         tCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACgg             >  4:38846/1‑149 (MQ=255)                                                                                                                                         tCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACgg             <  1:268090/149‑1 (MQ=255)                                                                                                                                         tCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACgg             <  1:179121/149‑1 (MQ=255)                                                                                                                                          cGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTGGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACGGc            <  5:55002/149‑1 (MQ=255)                                                                                                                                              gCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACGGCCCTg        >  3:70765/1‑149 (MQ=255)                                                                                                                                                cGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGc                                                        <  4:141003/99‑1 (MQ=255)                                                                                                                                                cGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGc                                                        >  3:141003/1‑99 (MQ=255)                                                                                                                                                cGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTc                                >  3:285569/1‑123 (MQ=255)                                                                                                                                                 ggCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACGGCCCTGCcg     <  5:47818/149‑1 (MQ=255)                                                                                                                                                   cTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACGGCCCTGCcgcg   <  6:317246/149‑1 (MQ=255)                                                                                                                                                   cTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACGGCCCTGCcgc    >  6:112623/1‑148 (MQ=255)                                                                                                                                                   cTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACGGCCCTGCcgc    <  5:112623/148‑1 (MQ=255)                                                                                                                                                    ttCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACGGCCCTGCCGCgg  >  8:175979/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      AAGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAT‑GACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACGGCCCTGCCGCGG  >  NC_002947/5711717‑5712012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑