Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,715,386	T→A	6.7%	L18Q (CTG→CAG)	tatB →	Sec‑independent protein translocase protein TatB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,715,386	0	T	A	6.7%	172.8 / 2.5	60	L18Q (CTG→CAG)	tatB	Sec‑independent protein translocase protein TatB
Reads supporting (aligned to +/- strand):  ref base T (31/25);  new base A (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

CCACCGGTGCAAAACACCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAAC  >  NC_002947/5715241‑5715528                                                                                                                                                  |                                                                                                                                                ccACCGGTGCAAAACACCGCGCAGCCGCAGCAAGGCCACACCATTGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGCCCCATGTTCGGCTTGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGt                                                                                                                                              >  5:207638/1‑149 (MQ=255) ccACCGGTGCAAAACACCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGt                                                                                                                                              >  2:195770/1‑149 (MQ=255)  cACCGGTGCAAAACACCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTg                                                                                                                                             >  5:291847/1‑149 (MQ=255)  cACCGGTGCAAAACACCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTg                                                                                                                                             >  6:305418/1‑149 (MQ=255)   aCCGGTGCAAAACACCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGc                                                                                                                                            >  5:8430/1‑149 (MQ=255)     cGGTGCAAAACACCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTc                                                                                                                                          >  5:189014/1‑149 (MQ=255)               cacCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAgc                                                                                                                                <  6:8430/149‑1 (MQ=255)                 ccGCGCAGCCGCAGCAAGGCCAAACAAACGAAGGCAAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAgcgc                                                                                                                              >  6:111645/1‑149 (MQ=255)                      cagccgcagcAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCACCTGcc                                                                                                                         >  6:287021/1‑149 (MQ=255)                       agccgcagcAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGGGCCGAGCACCAGcag                                                                                                                        <  1:152125/149‑3 (MQ=255)                       acccgcagcAAGG‑CACCCCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCAGGCGCTCGGGGCCGAGCACCAGCAgg                                                                                                                       <  1:292294/147‑1 (MQ=255)                         ccgcagcAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCggg                                                                                                                      >  6:226473/1‑149 (MQ=255)                           gcagcaAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTg                                                                                                                    >  7:121457/1‑149 (MQ=255)                             agcaAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGcc                                                                                                                  >  2:123840/1‑149 (MQ=255)                              gcaAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAGGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCcg                                                                                                                 >  8:34909/1‑149 (MQ=255)                                    ccACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑a                                                                                                          <  6:291847/149‑1 (MQ=255)                                          cATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCGGCAGGCGCTCGGGGCCGAGCACCAGCAggg                                                                                                                      >  7:90404/1‑132 (MQ=38)                                          cATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCGGCAGGCGCTCGGGGCCGAGCACCAGCAggg                                                                                                                      <  8:90404/132‑1 (MQ=38)                                            tCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAggg                                                                                                  <  1:195770/149‑1 (MQ=255)                                               aaGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtg                                                                                               >  6:293446/1‑149 (MQ=255)                                                aggccaggccCACCCGGTCCAGGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAggcgctc                                                                                              <  7:34909/149‑6 (MQ=255)                                                aggccaggccCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                              <  6:189014/149‑1 (MQ=255)                                                aggccaggccCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                              <  3:18279/149‑1 (MQ=255)                                                  gccaggccCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTgg                                                                                            <  7:83762/149‑1 (MQ=255)                                                    caggccCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGAt                                                                                          >  1:168418/1‑149 (MQ=255)                                                        cccACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATcggc                                                                                      <  5:293446/149‑1 (MQ=255)                                                         ccACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCa                                                                                                     <  1:253434/133‑1 (MQ=255)                                                         ccACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCa                                                                                                     >  2:253434/1‑133 (MQ=255)                                                                   cAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACg                                                                           >  3:82429/1‑149 (MQ=255)                                                                      gagCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                              >  3:251009/1‑127 (MQ=255)                                                                      gagCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                              <  4:251009/127‑1 (MQ=255)                                                                       agCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGc                                                                       <  5:305418/149‑1 (MQ=255)                                                                                ggAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAg                                                               >  3:230160/1‑148 (MQ=255)                                                                                  aaaGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGAACGGCCGGCTCAAACGCAGCTTCAACAGCa                                                             >  7:138108/1‑148 (MQ=255)                                                                                   aaGACTGACCCATGTTCGGCATGAGTTTCAGCGAGTTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATc                                                           <  6:283731/149‑1 (MQ=255)                                                                                   aaGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATc                                                           <  2:38157/149‑1 (MQ=255)                                                                                      aCTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATc                                                           >  4:49955/1‑146 (MQ=255)                                                                                          aCCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                              >  4:75933/1‑107 (MQ=255)                                                                                          aCCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                              <  3:75933/107‑1 (MQ=255)                                                                                              aTGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGt                                                >  8:302148/1‑149 (MQ=255)                                                                                               tGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTg                                               <  7:302148/149‑1 (MQ=255)                                                                                                gTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTgg                                              >  3:102093/1‑149 (MQ=255)                                                                                                 ttCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGTAAGTg                                               >  7:116263/1‑147 (MQ=255)                                                                                                   cGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAgc                                           >  5:163334/1‑149 (MQ=255)                                                                                                            tttCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGa                                             >  4:226973/1‑138 (MQ=255)                                                                                                            tttCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGa                                             <  3:226973/138‑1 (MQ=255)                                                                                                                aGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAgcgc                                         <  3:38684/138‑1 (MQ=255)                                                                                                                aGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAgcgc                                         >  4:38684/1‑138 (MQ=255)                                                                                                                       tgctgctCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCgacgac                       <  8:121457/149‑1 (MQ=255)                                                                                                                            ctCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCACCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATccg                  <  5:287021/149‑1 (MQ=255)                                                                                                                                tcgGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGCAATAg                                  <  7:292785/129‑1 (MQ=255)                                                                                                                                tcgGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAg                                  >  8:292785/1‑129 (MQ=255)                                                                                                                                tcgGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCcgccgc              <  1:214282/149‑1 (MQ=255)                                                                                                                                   gCCTGGTCGCCCTGCT‑GTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAAc          >  6:54218/1‑149 (MQ=255)                                                                                                                                     cTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACt         <  1:123840/149‑1 (MQ=255)                                                                                                                                       ggTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGCAACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTg        >  1:80148/1‑149 (MQ=255)                                                                                                                                        gTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCTC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGc       <  8:138108/148‑1 (MQ=255)                                                                                                                                           gCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATaa   >  2:214247/1‑149 (MQ=255)                                                                                                                                           gCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATa    <  6:163334/148‑1 (MQ=255)                                                                                                                                            cccGGCAGGCGCTCGGGGCCGAGCACCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAAc  >  3:293967/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                CCACCGGTGCAAAACACCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAAC  >  NC_002947/5715241‑5715528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑