Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,715,404	G→A	12.0%	R24H (CGC→CAC)	tatB →	Sec‑independent protein translocase protein TatB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,715,404	0	G	A	12.0%	148.4 / 9.3	58	R24H (CGC→CAC)	tatB	Sec‑independent protein translocase protein TatB
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base A (3/4);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-01

CCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAACGAGCACATCCTGCAGATGGAAGA  >  NC_002947/5715257‑5715551                                                                                                                                                    |                                                                                                                                                     ccGCGCAGCCGCAGCAAGGCCAAACAAACGAAGGCAAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAgcgc                                                                                                                                                     >  6:111645/1‑149 (MQ=255)      cagccgcagcAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCACCTGcc                                                                                                                                                >  6:287021/1‑149 (MQ=255)       agccgcagcAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGGGCCGAGCACCAGcag                                                                                                                                               <  1:152125/149‑3 (MQ=255)       acccgcagcAAGG‑CACCCCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCAGGCGCTCGGGGCCGAGCACCAGCAgg                                                                                                                                              <  1:292294/147‑1 (MQ=255)         ccgcagcAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCggg                                                                                                                                             >  6:226473/1‑149 (MQ=255)           gcagcaAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTg                                                                                                                                           >  7:121457/1‑149 (MQ=255)             agcaAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGcc                                                                                                                                         >  2:123840/1‑149 (MQ=255)              gcaAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAGGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCcg                                                                                                                                        >  8:34909/1‑149 (MQ=255)                    ccACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑a                                                                                                                                 <  6:291847/149‑1 (MQ=255)                          cATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCGGCAGGCGCTCGGGGCCGAGCACCAGCAggg                                                                                                                                             <  8:90404/132‑1 (MQ=38)                          cATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCGGCAGGCGCTCGGGGCCGAGCACCAGCAggg                                                                                                                                             >  7:90404/1‑132 (MQ=38)                            tCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAggg                                                                                                                         <  1:195770/149‑1 (MQ=255)                               aaGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtg                                                                                                                      >  6:293446/1‑149 (MQ=255)                                aggccaggccCACCCGGTCCAGGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAggcgctc                                                                                                                     <  7:34909/149‑6 (MQ=255)                                aggccaggccCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                                                     <  6:189014/149‑1 (MQ=255)                                aggccaggccCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                                                     <  3:18279/149‑1 (MQ=255)                                  gccaggccCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTgg                                                                                                                   <  7:83762/149‑1 (MQ=255)                                    caggccCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGAt                                                                                                                 >  1:168418/1‑149 (MQ=255)                                        cccACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATcggc                                                                                                             <  5:293446/149‑1 (MQ=255)                                         ccACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCa                                                                                                                            >  2:253434/1‑133 (MQ=255)                                         ccACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCa                                                                                                                            <  1:253434/133‑1 (MQ=255)                                                   cAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACg                                                                                                  >  3:82429/1‑149 (MQ=255)                                                      gagCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                                                     <  4:251009/127‑1 (MQ=255)                                                      gagCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                                                     >  3:251009/1‑127 (MQ=255)                                                       agCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGc                                                                                              <  5:305418/149‑1 (MQ=255)                                                                ggAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAg                                                                                      >  3:230160/1‑148 (MQ=255)                                                                  aaaGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGAACGGCCGGCTCAAACGCAGCTTCAACAGCa                                                                                    >  7:138108/1‑148 (MQ=255)                                                                   aaGACTGACCCATGTTCGGCATGAGTTTCAGCGAGTTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATc                                                                                  <  6:283731/149‑1 (MQ=255)                                                                   aaGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATc                                                                                  <  2:38157/149‑1 (MQ=255)                                                                      aCTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATc                                                                                  >  4:49955/1‑146 (MQ=255)                                                                          aCCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                                                     >  4:75933/1‑107 (MQ=255)                                                                          aCCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCtgt                                                                                                                     <  3:75933/107‑1 (MQ=255)                                                                              aTGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGt                                                                       >  8:302148/1‑149 (MQ=255)                                                                               tGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTg                                                                      <  7:302148/149‑1 (MQ=255)                                                                                gTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTgg                                                                     >  3:102093/1‑149 (MQ=255)                                                                                 ttCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGTAAGTg                                                                      >  7:116263/1‑147 (MQ=255)                                                                                   cGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAgc                                                                  >  5:163334/1‑149 (MQ=255)                                                                                            tttCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGa                                                                    <  3:226973/138‑1 (MQ=255)                                                                                            tttCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGa                                                                    >  4:226973/1‑138 (MQ=255)                                                                                                aGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAgcgc                                                                >  4:38684/1‑138 (MQ=255)                                                                                                aGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAgcgc                                                                <  3:38684/138‑1 (MQ=255)                                                                                                       tgctgctCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCgacgac                                              <  8:121457/149‑1 (MQ=255)                                                                                                            ctCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCACCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATccg                                         <  5:287021/149‑1 (MQ=255)                                                                                                                tcgGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGCAATAg                                                         <  7:292785/129‑1 (MQ=255)                                                                                                                tcgGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAg                                                         >  8:292785/1‑129 (MQ=255)                                                                                                                tcgGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCcgccgc                                     <  1:214282/149‑1 (MQ=255)                                                                                                                   gCCTGGTCGCCCTGCT‑GTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAAc                                 >  6:54218/1‑149 (MQ=255)                                                                                                                     cTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACt                                <  1:123840/149‑1 (MQ=255)                                                                                                                       ggTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGCAACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTg                               >  1:80148/1‑149 (MQ=255)                                                                                                                        gTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCTC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGc                              <  8:138108/148‑1 (MQ=255)                                                                                                                           gCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATaa                          >  2:214247/1‑149 (MQ=255)                                                                                                                           gCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATa                           <  6:163334/148‑1 (MQ=255)                                                                                                                            cccGGCAGGCGCTCGGGGCCGAGCACCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAAc                         >  3:293967/1‑149 (MQ=255)                                                                                                                                     tGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAACGAGCACATc                <  2:112740/149‑1 (MQ=255)                                                                                                                                        tCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAAc                                 <  1:77678/129‑1 (MQ=255)                                                                                                                                        tCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAAc                                 >  2:77678/1‑129 (MQ=255)                                                                                                                                          ggCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAACGAGCACATCCTg             >  5:218768/1‑147 (MQ=255)                                                                                                                                          ggCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAACGAGCACATCCTg             <  4:102093/147‑1 (MQ=255)                                                                                                                                          ggCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAACGAGCACATCCTGCa           <  3:49955/149‑1 (MQ=255)                                                                                                                                                  gcgcCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGACGACGACATCCGCCGCCAACTGCATAACGAGCACATCCTGCAGATGGaa    <  1:240101/148‑1 (MQ=255)                                                                                                                                                  gcgcCTCCCGGGTGCCGCGCGC‑ACCGAAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGAGGAGCGCGAAATAGGCGCCGACGACATCCCCCGCCAACTGCATAACGAGCACATCCAGCAGATgg      >  2:240101/1‑146 (MQ=255)                                                                                                                                                   cgcCTGCCGGGTGCCGCGCGC‑CCGGCAGGGCTGGGGAGCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAACGAGCACATCCTGCAGATGGAaga  <  8:229601/149‑1 (MQ=255)                                                                                                                                                   cgcCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAACGAGCACATCCTGCAGATGGAaga  <  4:82429/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CCGCGCAGCCGCAGCAAGGCCACACCATCGAAGGCCAGGCCCACCCGGTCCAAGAGCCGCAGCGGAAAGACTGACCCATGTTCGGCATGAGTTTCAGCGAGCTGCTGCTCGTCGGCCTGGTCGCCCTGCTGGTGCTCGGCCCCGAGCGCCTGCCGGGTGCCGCGCGC‑ACGGCAGGGCTGTGGATCGGCCGGCTCAAACGCAGCTTCAACAGCATCAAGATGGAAGTGGAGCGCGAAATAGGCGCCGACGACATCCGCCGCCAACTGCATAACGAGCACATCCTGCAGATGGAAGA  >  NC_002947/5715257‑5715551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑