Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,753,486 C→A 7.1% intergenic (+47/‑319) glnA → / → glnL glutamine synthetase/two‑component system sensor histidine kinase/phosphatase GlnL/GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,753,4860CA 7.1% 175.2 / 3.3 56intergenic (+47/‑319)glnA/glnLglutamine synthetase/two‑component system sensor histidine kinase/phosphatase GlnL/GlnG
Reads supporting (aligned to +/- strand):  ref base C (25/27);  new base A (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GACGACTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTCTG  >  NC_002947/5753338‑5753633
                                                                                                                                                    |                                                                                                                                                   
gacgacTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCGGGAATAAGAGATGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGcctccgtc                                                                                                                                                     >  2:44134/1‑149 (MQ=255)
    acTTCATCGATGTCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGa                                                                                                                                                 <  1:188125/149‑1 (MQ=255)
    acTTCATCGATGTCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGa                                                                                                                                                 <  1:192930/149‑1 (MQ=255)
    acTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGa                                                                                                                                                 <  6:112381/149‑1 (MQ=255)
      ttCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCAGTACTACAGCAGCTGATCTGATTGGCGCCACGCGCCGATGACATGATCAAAACGGCCTCCCACTGGAgg                                                                                                                                               >  3:91407/1‑149 (MQ=255)
              tGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGttttt                                                                                                                                       <  2:106312/149‑1 (MQ=255)
                ccTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTg                                                                                                                                      <  3:151416/148‑1 (MQ=255)
                ccTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGt                                                                                                                                     <  5:295310/149‑1 (MQ=255)
                 cTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGtt                                                                                                                                          <  1:279728/143‑1 (MQ=255)
                 cTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGtt                                                                                                                                          >  2:279728/1‑143 (MQ=255)
                    cATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGttt                                                                                                                                   <  4:126677/147‑1 (MQ=255)
                         aGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGtt                                                                                                                                    <  2:222528/141‑1 (MQ=255)
                         aGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGtt                                                                                                                                    >  1:222528/1‑141 (MQ=255)
                               agagCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGc                                                                                                                      <  3:80909/149‑1 (MQ=255)
                                gagCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCa                                                                                                                     >  3:27388/1‑149 (MQ=255)
                                   cGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAg                                                                                                                    >  5:281061/1‑147 (MQ=255)
                                    gaagaagaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAg                                                                                                                 >  5:263393/1‑149 (MQ=255)
                                     aagaagaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAgc                                                                                                                >  5:29140/1‑149 (MQ=255)
                                         agaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGcgccg                                                                                                            >  8:104594/1‑149 (MQ=255)
                                            aaTCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCt                                                                                                          <  4:27388/148‑1 (MQ=255)
                                              tCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGAt                                                                                                       >  6:205284/1‑149 (MQ=255)
                                              tCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGAt                                                                                                       <  6:29140/149‑1 (MQ=255)
                                              tCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGAt                                                                                                       >  1:314835/1‑149 (MQ=255)
                                                aaGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCGtt                                                                                                                                          >  7:31510/1‑112 (MQ=255)
                                                aaGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCGtt                                                                                                                                          <  8:31510/112‑1 (MQ=255)
                                                   gTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGcc                                                                                                  >  2:207856/1‑149 (MQ=255)
                                                         aCCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCAc                                                                                            >  8:95459/1‑149 (MQ=255)
                                                         aCCTTCGTACACCCGCGGGAATCCGAGCTGTATTACAGGTGCTGATGTTATTGCCGCCTCGCGCCTATGACATGATCAAAACGGCCGCCCTCGGGTGGCGGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCa                                                                                             <  2:162157/148‑1 (MQ=255)
                                                          ccTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCAc                                                                                            <  7:104594/148‑1 (MQ=255)
                                                                tACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGc                                                                                     >  7:92921/1‑149 (MQ=255)
                                                                   acCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGGGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGctct                                                                                  <  3:52701/149‑1 (MQ=255)
                                                                   acCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTTACGCTATGctct                                                                                  <  5:268229/149‑1 (MQ=255)
                                                                   acCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGctct                                                                                  <  1:207856/149‑1 (MQ=255)
                                                                     ccGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGaa                                                                                                                  <  1:188502/115‑1 (MQ=255)
                                                                     ccGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGaa                                                                                                                  >  2:188502/1‑115 (MQ=255)
                                                                     ccGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTa                                                                                 >  8:18724/1‑148 (MQ=255)
                                                                           gAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTg                                                                            <  6:263393/147‑1 (MQ=255)
                                                                           gAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGt                                                                          <  5:205284/149‑1 (MQ=255)
                                                                                 gAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTa                                                                    <  8:92921/149‑1 (MQ=255)
                                                                                     tGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAAtt                                                                 >  6:85390/1‑148 (MQ=255)
                                                                                              gctgctGATCTGATTGGCGCCTCGCGCCGAAGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGc                                                       >  1:30523/1‑149 (MQ=255)
                                                                                               ctgctgATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGcc                                                      <  3:102387/149‑1 (MQ=255)
                                                                                                         gatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCt                                            >  8:253379/1‑149 (MQ=255)
                                                                                                          atTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTc                                           >  7:54095/1‑149 (MQ=255)
                                                                                                          atTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTc                                           >  6:180998/1‑149 (MQ=255)
                                                                                                           ttGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCAt                                                                      <  8:223208/121‑1 (MQ=255)
                                                                                                           ttGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCAt                                                                      >  7:223208/1‑121 (MQ=255)
                                                                                                           ttGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTcc                                          <  8:54095/149‑1 (MQ=255)
                                                                                                                gcCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCaa                                      >  6:152764/1‑148 (MQ=255)
                                                                                                                 ccTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATc                                    >  3:302099/1‑149 (MQ=255)
                                                                                                                               cATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATg                                                        >  4:286666/1‑115 (MQ=255)
                                                                                                                                aTGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAAc                     >  1:214553/1‑149 (MQ=255)
                                                                                                                                     taaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTc                <  7:206870/148‑1 (MQ=255)
                                                                                                                                      aaaaCGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCa               <  6:295196/149‑1 (MQ=255)
                                                                                                                                       aaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTc                >  8:206870/1‑147 (MQ=255)
                                                                                                                                        aaCGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCTCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCAcc             <  6:281061/149‑1 (MQ=255)
                                                                                                                                            gCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGa         <  2:30523/149‑1 (MQ=255)
                                                                                                                                                 cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTc    >  5:215551/1‑149 (MQ=255)
                                                                                                                                                 cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTc    <  5:180998/149‑1 (MQ=255)
                                                                                                                                                   tcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTCTg  >  8:203029/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GACGACTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTCTG  >  NC_002947/5753338‑5753633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑