Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,790,137	G→A	11.8%	intergenic (+16/+52)	ynfM → / ← PP_5073	efflux transporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,790,137	0	G	A	11.8%	155.4 / 9.4	59	intergenic (+16/+52)	ynfM/PP_5073	efflux transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/29);  new base A (3/4);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01

CCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTT  >  NC_002947/5789991‑5790281                                                                                                                                                       |                                                                                                                                                 ccGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGTTTGCTGGCAGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGcc                                                                                                                                                <  1:192188/149‑1 (MQ=255)          cgggcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCcac                                                                                                                                       <  4:43259/149‑1 (MQ=255)          cgggcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCca                                                                                                                                        >  2:32256/1‑148 (MQ=255)           gggcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCcac                                                                                                                                       >  2:136374/1‑148 (MQ=255)             gcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAAc                                                                                                                                    <  1:45349/149‑1 (MQ=255)             gcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAAc                                                                                                                                    >  6:169614/1‑149 (MQ=255)             gcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAAc                                                                                                                                    <  6:233683/149‑1 (MQ=255)                 gttgtTCTGGCACCAGTGGGGCTGGAAGGGGATCGTTCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCtgt                                                                                                                                <  7:225956/147‑1 (MQ=255)                     ttCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCAc                                                                                                                                           >  5:67255/1‑134 (MQ=255)                     ttCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCAc                                                                                                                                           <  6:67255/134‑1 (MQ=255)                     ttCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGa                                                                                                                            <  1:136374/149‑1 (MQ=255)                          gCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATGGCCTCTTCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGc                                                                                                                          <  5:200663/149‑1 (MQ=25)                            aCCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTAGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgtg                                                                                                                     >  6:242550/1‑149 (MQ=255)                             ccAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCGACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgtgt                                                                                                                    <  8:244045/149‑1 (MQ=255)                              cAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTACTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgtgt                                                                                                                    >  5:81660/1‑148 (MQ=255)                                 tGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGTCACACCCGCTCCCACAGGTTGTgggt                                                                                                                            <  5:71512/137‑2 (MQ=39)                                 tGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGTCACACCCGCTCCCACAGGTTGTgggt                                                                                                                            >  6:71512/1‑136 (MQ=39)                                 tGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGAc                                                                                                                 >  4:2144/1‑148 (MQ=255)                                   gggCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCAc                                                                                                              <  3:2144/149‑1 (MQ=255)                                         gAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAagag                                                                                                        >  3:197033/1‑149 (MQ=255)                                           aCGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGaatgcat                                                                                                      <  4:195812/149‑6 (MQ=25)                                               gATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCa                                                                                                               >  2:275613/1‑136 (MQ=255)                                               gATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCatc                                                                                                  <  8:32628/149‑1 (MQ=255)                                                       tGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGt                                                                                          >  4:212383/1‑149 (MQ=255)                                                           cATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtga                                                                                       <  4:197033/148‑1 (MQ=255)                                                            aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGaa                                                                                                           >  6:256908/1‑127 (MQ=255)                                                            aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGaa                                                                                                           <  5:256908/127‑1 (MQ=255)                                                            aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtgat                                                                                      >  6:272323/1‑148 (MQ=255)                                                            aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtgat                                                                                      >  7:178395/1‑148 (MQ=255)                                                                 cAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGAtctc                                                                                <  3:212383/149‑1 (MQ=255)                                                                     ttGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGCCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGATGCCATCAGAACAGTTGATGATCTCGAc                                                                             >  5:308272/1‑148 (MQ=255)                                                                           gCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatc                                                                       <  8:271879/148‑1 (MQ=255)                                                                                      gATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCATAAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACg                                                                                                             >  1:48096/1‑100 (MQ=255)                                                                                      gATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACg                                                                                                             <  2:48096/99‑1 (MQ=255)                                                                                       aTGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTcc                                                          <  1:32256/149‑1 (MQ=255)                                                                                       aTGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGGGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTcc                                                          >  4:138650/1‑149 (MQ=255)                                                                                          gtggCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCgg                                                       <  8:178395/149‑1 (MQ=255)                                                                                                   cACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAg                                               <  5:176099/148‑1 (MQ=255)                                                                                                         aggaaGTTGCCACCCT‑AAACGGTGTGTTG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCttt                                        <  6:308272/146‑1 (MQ=255)                                                                                                           cAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCtgtt                                       >  6:256974/1‑148 (MQ=255)                                                                                                             aGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCaa                                    <  5:169614/149‑1 (MQ=255)                                                                                                             aGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCaa                                    >  6:236617/1‑149 (MQ=255)                                                                                                                     cccA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCg                            >  8:116832/1‑149 (MQ=255)                                                                                                                     cccA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCg                            <  7:116832/149‑1 (MQ=255)                                                                                                                      ccA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCgg                           >  8:280810/1‑149 (MQ=255)                                                                                                                           aaCGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAg                       <  5:242550/149‑1 (MQ=255)                                                                                                                                   gATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCt               >  2:74986/1‑149 (MQ=255)                                                                                                                                     tG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTg             <  5:236617/149‑1 (MQ=255)                                                                                                                                      ggcctcttCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGc                      >  2:221359/7‑142 (MQ=21)                                                                                                                                      ggcctcttCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGc                      <  1:221359/136‑1 (MQ=21)                                                                                                                                      g‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTgg            >  8:176388/1‑149 (MQ=255)                                                                                                                                         ctcttCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATACACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCtttg            >  8:264502/4‑147 (MQ=25)                                                                                                                                           aTTCGTTGTCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGt           <  8:221868/148‑1 (MQ=255)                                                                                                                                           aTTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatcca                                                                     <  4:35917/90‑1 (MQ=255)                                                                                                                                              cGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCtt        <  6:81660/148‑1 (MQ=255)                                                                                                                                               gTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGt      <  7:105355/149‑1 (MQ=255)                                                                                                                                                tGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTc     <  6:71870/149‑1 (MQ=255)                                                                                                                                                   gCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGtt  <  5:272323/149‑1 (MQ=255)                                                                                                                                                   gCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAAGATCCACATCCACTTCCCGGCTCCTCAGGTGCTTTTCAACCTCACCGGTCGGCTTGACATTGGTCTTGTCggt  >  5:206947/1‑147 (MQ=255)                                                                                                                                                       |                                                                                                                                                 CCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTT  >  NC_002947/5789991‑5790281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑