Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,790,143	A→T	11.8%	intergenic (+22/+46)	ynfM → / ← PP_5073	efflux transporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,790,143	0	A	T	11.8%	160.6 / 9.7	59	intergenic (+22/+46)	ynfM/PP_5073	efflux transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/28);  new base T (3/4);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGG  >  NC_002947/5790000‑5790287                                                                                                                                                    |                                                                                                                                                 cgggcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCcac                                                                                                                                             <  4:43259/149‑1 (MQ=255) cgggcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCca                                                                                                                                              >  2:32256/1‑148 (MQ=255)  gggcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCcac                                                                                                                                             >  2:136374/1‑148 (MQ=255)    gcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAAc                                                                                                                                          <  6:233683/149‑1 (MQ=255)    gcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAAc                                                                                                                                          <  1:45349/149‑1 (MQ=255)    gcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAAc                                                                                                                                          >  6:169614/1‑149 (MQ=255)        gttgtTCTGGCACCAGTGGGGCTGGAAGGGGATCGTTCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCtgt                                                                                                                                      <  7:225956/147‑1 (MQ=255)            ttCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCAc                                                                                                                                                 <  6:67255/134‑1 (MQ=255)            ttCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCAc                                                                                                                                                 >  5:67255/1‑134 (MQ=255)            ttCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGa                                                                                                                                  <  1:136374/149‑1 (MQ=255)                 gCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATGGCCTCTTCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGc                                                                                                                                <  5:200663/149‑1 (MQ=25)                   aCCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTAGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgtg                                                                                                                           >  6:242550/1‑149 (MQ=255)                    ccAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCGACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgtgt                                                                                                                          <  8:244045/149‑1 (MQ=255)                     cAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTACTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgtgt                                                                                                                          >  5:81660/1‑148 (MQ=255)                        tGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGTCACACCCGCTCCCACAGGTTGTgggt                                                                                                                                  <  5:71512/137‑2 (MQ=39)                        tGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGTCACACCCGCTCCCACAGGTTGTgggt                                                                                                                                  >  6:71512/1‑136 (MQ=39)                        tGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGAc                                                                                                                       >  4:2144/1‑148 (MQ=255)                          gggCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCAc                                                                                                                    <  3:2144/149‑1 (MQ=255)                                gAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAagag                                                                                                              >  3:197033/1‑149 (MQ=255)                                  aCGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGaatgcat                                                                                                            <  4:195812/149‑6 (MQ=25)                                      gATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCa                                                                                                                     >  2:275613/1‑136 (MQ=255)                                      gATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCatc                                                                                                        <  8:32628/149‑1 (MQ=255)                                              tGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGt                                                                                                >  4:212383/1‑149 (MQ=255)                                                  cATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtga                                                                                             <  4:197033/148‑1 (MQ=255)                                                   aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGaa                                                                                                                 >  6:256908/1‑127 (MQ=255)                                                   aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGaa                                                                                                                 <  5:256908/127‑1 (MQ=255)                                                   aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtgat                                                                                            >  6:272323/1‑148 (MQ=255)                                                   aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtgat                                                                                            >  7:178395/1‑148 (MQ=255)                                                        cAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGAtctc                                                                                      <  3:212383/149‑1 (MQ=255)                                                            ttGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGCCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGATGCCATCAGAACAGTTGATGATCTCGAc                                                                                   >  5:308272/1‑148 (MQ=255)                                                                  gCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatc                                                                             <  8:271879/148‑1 (MQ=255)                                                                             gATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCATAAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACg                                                                                                                   >  1:48096/1‑100 (MQ=255)                                                                             gATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACg                                                                                                                   <  2:48096/99‑1 (MQ=255)                                                                              aTGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTcc                                                                <  1:32256/149‑1 (MQ=255)                                                                              aTGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGGGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTcc                                                                >  4:138650/1‑149 (MQ=255)                                                                                 gtggCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCgg                                                             <  8:178395/149‑1 (MQ=255)                                                                                          cACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAg                                                     <  5:176099/148‑1 (MQ=255)                                                                                                aggaaGTTGCCACCCT‑AAACGGTGTGTTG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCttt                                              <  6:308272/146‑1 (MQ=255)                                                                                                  cAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCtgtt                                             >  6:256974/1‑148 (MQ=255)                                                                                                    aGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCaa                                          >  6:236617/1‑149 (MQ=255)                                                                                                    aGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCaa                                          <  5:169614/149‑1 (MQ=255)                                                                                                            cccA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCg                                  <  7:116832/149‑1 (MQ=255)                                                                                                            cccA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCg                                  >  8:116832/1‑149 (MQ=255)                                                                                                             ccA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCgg                                 >  8:280810/1‑149 (MQ=255)                                                                                                                  aaCGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAg                             <  5:242550/149‑1 (MQ=255)                                                                                                                          gATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCt                     >  2:74986/1‑149 (MQ=255)                                                                                                                            tG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTg                   <  5:236617/149‑1 (MQ=255)                                                                                                                             ggcctcttCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGc                            <  1:221359/136‑1 (MQ=21)                                                                                                                             ggcctcttCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGc                            >  2:221359/7‑142 (MQ=21)                                                                                                                             g‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTgg                  >  8:176388/1‑149 (MQ=255)                                                                                                                                ctcttCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATACACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCtttg                  >  8:264502/4‑147 (MQ=25)                                                                                                                                  aTTCGTTGTCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGt                 <  8:221868/148‑1 (MQ=255)                                                                                                                                  aTTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatcca                                                                           <  4:35917/90‑1 (MQ=255)                                                                                                                                     cGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCtt              <  6:81660/148‑1 (MQ=255)                                                                                                                                      gTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGt            <  7:105355/149‑1 (MQ=255)                                                                                                                                       tGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTc           <  6:71870/149‑1 (MQ=255)                                                                                                                                          gCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGtt        <  5:272323/149‑1 (MQ=255)                                                                                                                                          gCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAAGATCCACATCCACTTCCCGGCTCCTCAGGTGCTTTTCAACCTCACCGGTCGGCTTGACATTGGTCTTGTCggt        >  5:206947/1‑147 (MQ=255)                                                                                                                                                ccGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACgg  >  6:3416/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCA‑AAACGGTGTGATG‑‑C‑ATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGG  >  NC_002947/5790000‑5790287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑