Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,792,846 A→C 6.7% L162R (CTG→CGG)  gltD ← glutamate synthase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,792,8460AC 6.7% 175.4 / 2.4 59L162R (CTG→CGG) gltDglutamate synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (32/23);  new base C (2/2);  total (34/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGTTCA  >  NC_002947/5792699‑5792993
                                                                                                                                                   |                                                                                                                                                   
ccGGTGAACACTTCACGGCGGTTGTTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAg                                                                                                                                                    <  2:237297/149‑1 (MQ=255)
           ttCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggc                                                                                                                                         >  5:322014/1‑149 (MQ=255)
             cACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggccg                                                                                                                                       >  4:64092/1‑149 (MQ=255)
               gggcggTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACAGATCTCAGGGGTTTTGTCGAACACCACCGTGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGgc                                                                                                                                     <  2:103812/148‑1 (MQ=255)
               cggcggTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGgc                                                                                                                                     <  3:233812/149‑1 (MQ=255)
                 gcggTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAATGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGTGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCCGGCCCggcgggcctgggc                                                                                                                                   <  8:153651/149‑6 (MQ=255)
                      tGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgac                                                                                                                               >  8:218742/1‑147 (MQ=38)
                      tGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgac                                                                                                                               <  7:218742/148‑2 (MQ=38)
                       gCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCgatga                                                                                                                             >  2:91501/1‑149 (MQ=255)
                        cTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCgatgat                                                                                                                            <  8:171524/149‑1 (MQ=255)
                           aGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATgg                                                                                                                          >  6:287167/1‑148 (MQ=255)
                              aCGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAc                                                                                                                      <  1:91501/149‑1 (MQ=255)
                                ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                                    >  1:68525/1‑149 (MQ=255)
                                         cAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGTCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCgg                                                                                                           >  4:178284/1‑149 (MQ=255)
                                          aGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGt                                                                                                          >  8:128526/1‑149 (MQ=255)
                                           gCTTGAACTCGGGGATACCGAAGGTCAGCCGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGtt                                                                                                         <  5:287167/149‑1 (MQ=255)
                                           gCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGtt                                                                                                         <  1:16784/149‑1 (MQ=255)
                                               gAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTgg                                                                                                       <  1:316096/147‑1 (MQ=255)
                                                aaCTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCtt                                                                                                    >  7:131957/1‑149 (MQ=255)
                                                aaCTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCtt                                                                                                    <  7:222312/149‑1 (MQ=255)
                                                         aTACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGa                                                                                           <  2:68525/149‑1 (MQ=255)
                                                          tACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGAc                                                                                          <  3:91138/149‑1 (MQ=255)
                                                             cGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATg                                                                                       >  2:84438/1‑149 (MQ=255)
                                                             cGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATg                                                                                       >  6:210039/1‑149 (MQ=255)
                                                             cGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATg                                                                                       >  8:250163/1‑149 (MQ=255)
                                                                     agcagGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACg                                                                               >  5:319351/1‑149 (MQ=255)
                                                                     agcagGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACg                                                                               >  5:172837/1‑149 (MQ=255)
                                                                         ggCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAg                                                                           <  7:128526/149‑1 (MQ=255)
                                                                            cACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGTCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGccc                                                                        <  3:178284/149‑1 (MQ=255)
                                                                            cACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAAGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGcc                                                                         >  4:118872/1‑148 (MQ=255)
                                                                                      aGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAgg                                                              <  7:250163/149‑1 (MQ=255)
                                                                                      aGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAg                                                               <  6:172837/148‑1 (MQ=255)
                                                                                        ggTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCg                                                            >  8:119314/1‑149 (MQ=255)
                                                                                        ggTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCg                                                            >  2:50193/1‑149 (MQ=255)
                                                                                        ggTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCg                                                            <  7:119314/149‑1 (MQ=255)
                                                                                        ggTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCg                                                            <  3:64092/149‑1 (MQ=255)
                                                                                        ggTTTTTGTCGAACACCACCGGGGTTACCCCCGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCg                                                            >  3:181908/1‑149 (MQ=255)
                                                                                          tttttGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGgt                                                          <  8:131957/149‑1 (MQ=255)
                                                                                               gTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCgg                                                     >  6:280198/1‑149 (MQ=255)
                                                                                                 cGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTg                                                   >  3:28608/1‑149 (MQ=255)
                                                                                                  gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                  >  4:234197/1‑149 (MQ=255)
                                                                                                  gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                  >  6:46847/1‑149 (MQ=255)
                                                                                                    acacCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATg                                                >  3:172799/1‑149 (MQ=255)
                                                                                                     caccacCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGt                                               <  1:84438/149‑1 (MQ=255)
                                                                                                            ggggTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTtctc                                        <  3:235654/149‑1 (MQ=255)
                                                                                                             gggTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTtctc                                        >  4:312356/1‑148 (MQ=255)
                                                                                                             gggTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTcc                                       <  2:99286/149‑1 (MQ=255)
                                                                                                                ttACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGGCATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCAcc                                    >  6:198646/1‑149 (MQ=255)
                                                                                                                    cccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGc                                >  5:71711/1‑149 (MQ=255)
                                                                                                                         cgcgcACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATg                           <  3:234197/149‑1 (MQ=255)
                                                                                                                                cAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGGCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcg                    >  2:146898/1‑149 (MQ=255)
                                                                                                                                      gTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTNCGGACGCCAGCCCATGGCGAAAGCGGTGTAGGTGATGTACTTCTCCACCGAGCCGATGGTCCCCGCGCCg               >  1:104615/1‑148 (MQ=255)
                                                                                                                                       tCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGa              >  1:146801/1‑148 (MQ=255)
                                                                                                                                       tCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGa              >  4:295889/1‑148 (MQ=255)
                                                                                                                                        cGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGaaa            >  8:177421/1‑149 (MQ=255)
                                                                                                                                          gcgcAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAcc          >  3:126032/1‑149 (MQ=255)
                                                                                                                                            gcAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCTCGCCGAAACcgt        >  8:64147/1‑149 (MQ=255)
                                                                                                                                                 ccAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGtt    >  5:246734/1‑148 (MQ=255)
                                                                                                                                                  cAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGTTCa  <  4:181908/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGTTCA  >  NC_002947/5792699‑5792993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑