Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,793,873 T→G 7.1% T1343P (ACT→CCT)  gltB ← L‑glutamate synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,793,8730TG 7.1% 214.8 / 7.0 70T1343P (ACT→CCT) gltBL‑glutamate synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (34/31);  new base G (2/3);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CCGCCAGTCATGCCAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGG  >  NC_002947/5793725‑5794019
                                                                                                                                                     |                                                                                                                                                  
ccgccAGTCATGCCAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGt                                                                                                                                                    >  4:275057/1‑149 (MQ=255)
      gTCATGCCAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGc                                                                                                                                              <  2:52106/149‑1 (MQ=255)
      gTCATGCCAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGc                                                                                                                                              >  1:52106/1‑149 (MQ=255)
            ccAGAACCGAAGTTGTAACCGGTCTTGCCCATAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcga                                                                                                                                        <  8:212158/149‑5 (MQ=255)
            ccAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCaaa                                                                                                                                        <  6:255199/149‑1 (MQ=255)
               gAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCAGGTCATGTATTCACAGAAGTGATCGCCAGTGCCCTCGACAACAGCATTGGCGCCGGAGTTACGTACATCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAg                                                                                                                                     >  1:54735/1‑149 (MQ=255)
                   cGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCCTCGCCCGCAGTACCTGCGGCAAACAGCtt                                                                                                                                  <  1:29882/149‑1 (MQ=255)
                   cGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTg                                                                                                                                 >  2:249552/1‑149 (MQ=255)
                    gAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTgc                                                                                                                                >  3:288874/1‑149 (MQ=255)
                    gAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcga                                                                                                                                        >  3:177227/1‑137 (MQ=255)
                    gAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcga                                                                                                                                        <  4:177227/141‑5 (MQ=255)
                          gTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAGCTGTTTgccgcag                                                                                                                           <  8:5516/148‑3 (MQ=255)
                          gTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCa                                                                                                                           <  1:249552/148‑1 (MQ=255)
                           tAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAg                                                                                                                          <  2:152270/148‑1 (MQ=255)
                           tAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAg                                                                                                                          <  1:223798/148‑1 (MQ=255)
                             aCCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTgg                                                                                                                       <  6:219620/149‑1 (MQ=255)
                             aCCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTgg                                                                                                                       >  3:199635/1‑149 (MQ=255)
                               cGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCACTGGCa                                                                                                                     >  4:107982/1‑149 (MQ=255)
                                        ccAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTAcagg                                                                                                            <  8:265706/149‑1 (MQ=255)
                                         cAGAACGCAGACAAAGCCGCCGTTCATGTATTCACAGGAGTTATCGCCAGTGCCCTCGACAACAGGATCGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTAcagg                                                                                                            <  3:187529/148‑1 (MQ=255)
                                             aCGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcgag                                                                                                                                       <  6:98185/117‑6 (MQ=255)
                                             aCGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcgag                                                                                                                                       >  5:98185/1‑112 (MQ=255)
                                               gCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGgtgt                                                                                                     >  4:135198/1‑149 (MQ=255)
                                                cAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGACAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCTCAGTAACTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACATGCAGGTGtt                                                                                                    <  6:75390/149‑1 (MQ=255)
                                                         ccgccgGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGAt                                                                                           >  4:84304/1‑149 (MQ=255)
                                                           gccgGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATgg                                                                                         >  4:283952/1‑149 (MQ=255)
                                                              ggTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGgcgc                                                                                      >  2:195870/1‑149 (MQ=255)
                                                                   tGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGc                                                                                 <  1:195870/149‑1 (MQ=255)
                                                                          acaGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGtt                                                                                                    >  5:183858/1‑123 (MQ=255)
                                                                          acaGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGtt                                                                                                    <  6:183858/123‑1 (MQ=255)
                                                                          acaGCAGTGATCGCCAGTGCCCTCGACAACAGAATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCAGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGttt                                                                          >  5:229389/1‑149 (MQ=255)
                                                                              cagTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAg                                                                                                                                     >  8:102805/1‑86 (MQ=255)
                                                                              cagTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAg                                                                                                                                     <  7:102805/86‑1 (MQ=255)
                                                                                gTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACg                                                                    <  1:142911/149‑1 (MQ=255)
                                                                                  gATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTgc                                                                                                                                >  5:72767/1‑87 (MQ=255)
                                                                                  gATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTgc                                                                                                                                <  6:72767/87‑1 (MQ=255)
                                                                                   aTCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACggg                                                                  <  7:284703/148‑1 (MQ=255)
                                                                                   aTCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGc                                                                 >  3:244498/1‑149 (MQ=255)
                                                                                       ccAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGcc                                                             >  2:200923/1‑149 (MQ=255)
                                                                                           tGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCt                                                         <  3:107982/149‑1 (MQ=255)
                                                                                              ccTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAATCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTggc                                                      >  7:71953/1‑149 (MQ=255)
                                                                                              ccTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTggc                                                      >  2:273952/1‑149 (MQ=255)
                                                                                                tCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCt                                                                                     <  4:30955/116‑1 (MQ=255)
                                                                                                tCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCt                                                                                     >  3:30955/1‑116 (MQ=255)
                                                                                                tCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTggcgg                                                    >  5:13360/1‑149 (MQ=255)
                                                                                                     aacaGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACggg                                                                  <  5:96238/130‑1 (MQ=255)
                                                                                                     aacaGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACggg                                                                  >  6:96238/1‑130 (MQ=255)
                                                                                                       caGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATg                                             <  1:273952/149‑1 (MQ=255)
                                                                                                       caGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATg                                             <  3:135198/149‑1 (MQ=255)
                                                                                                                       gTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCgg                             <  3:283952/149‑1 (MQ=255)
                                                                                                                          aCGAACAGCGAAGCG‑CTCGCCCGCAGTAACTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGCCAGATGTTGACGACGATGGCGCTGAGCTAGGTTTCGAACGCGCTGCCGGCAGGCGGCACGAAGGTGACCTTGCCACCGCTCa                          >  6:308325/1‑149 (MQ=255)
                                                                                                                           cGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCAt                         <  4:199635/149‑1 (MQ=255)
                                                                                                                              aCAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTg                                          <  6:237961/129‑1 (MQ=255)
                                                                                                                              aCAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTg                                          >  5:237961/1‑129 (MQ=255)
                                                                                                                               cAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCt                     >  4:290196/1‑149 (MQ=255)
                                                                                                                               cAGCGAAGAG‑AGCGCCCGCAGTACCTGCGGCAAACAGCGTGCAGACAGTGTAACCTTACAGGAAGGTTTTGCCTACTATGGCGCTGTGCTGTGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGAACTTGCCACCTGTCATGcc                      <  2:54735/148‑1 (MQ=255)
                                                                                                                                aGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCtt                    >  3:294594/1‑149 (MQ=255)
                                                                                                                                aGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCtt                    >  1:146311/1‑149 (MQ=255)
                                                                                                                                aGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCtt                    >  2:325538/1‑149 (MQ=255)
                                                                                                                                 gCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCttt                   >  5:103021/1‑149 (MQ=255)
                                                                                                                                 gCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCttt                   >  1:229118/1‑149 (MQ=255)
                                                                                                                                   gAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGc                 <  6:103021/149‑1 (MQ=255)
                                                                                                                                        g‑cTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGc                       >  7:155998/1‑138 (MQ=255)
                                                                                                                                        g‑cTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGc                       <  8:155998/138‑1 (MQ=255)
                                                                                                                                        g‑cTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACg            >  6:272658/1‑149 (MQ=255)
                                                                                                                                          cTCGCACGCACTACCTGCGCGAAACACCTTGCCGCCAGTGGCACCGTACAGGGAGCTCTTCCCGCCGCTGCCGCTGTGCTGGCTTTCGCAAGGGCTGACTGCTCCCCCCACGATGGTGGCATTGCCACCGGTAATGCCTTTGCCGACg            >  2:310691/1‑148 (MQ=255)
                                                                                                                                           tCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGt           >  5:64048/1‑148 (MQ=255)
                                                                                                                                                  cAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTg   <  1:200923/149‑1 (MQ=255)
                                                                                                                                                   aGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTgg  <  1:270396/149‑1 (MQ=255)
                                                                                                                                                   aGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTg   >  4:49951/1‑148 (MQ=255)
                                                                                                                                                   aGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTg   <  3:49951/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCGCCAGTCATGCCAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGG  >  NC_002947/5793725‑5794019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑