Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,793,874	A→T	6.6%	G1342G (GGT→GGA) ‡	gltB ←	L‑glutamate synthase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,793,874	0	A	T	6.6%	213.1 / 3.4	70	G1342G (GGT→GGA) ‡	gltB	L‑glutamate synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base T (2/3);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCATGCCAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGG  >  NC_002947/5793731‑5794019                                                                                                                                                 |                                                                                                                                                  gTCATGCCAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGc                                                                                                                                              >  1:52106/1‑149 (MQ=255) gTCATGCCAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGc                                                                                                                                              <  2:52106/149‑1 (MQ=255)       ccAGAACCGAAGTTGTAACCGGTCTTGCCCATAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcga                                                                                                                                        <  8:212158/149‑5 (MQ=255)       ccAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCaaa                                                                                                                                        <  6:255199/149‑1 (MQ=255)          gAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCAGGTCATGTATTCACAGAAGTGATCGCCAGTGCCCTCGACAACAGCATTGGCGCCGGAGTTACGTACATCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAg                                                                                                                                     >  1:54735/1‑149 (MQ=255)              cGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCCTCGCCCGCAGTACCTGCGGCAAACAGCtt                                                                                                                                  <  1:29882/149‑1 (MQ=255)              cGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTg                                                                                                                                 >  2:249552/1‑149 (MQ=255)               gAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTgc                                                                                                                                >  3:288874/1‑149 (MQ=255)               gAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcga                                                                                                                                        <  4:177227/141‑5 (MQ=255)               gAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcga                                                                                                                                        >  3:177227/1‑137 (MQ=255)                     gTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAGCTGTTTgccgcag                                                                                                                           <  8:5516/148‑3 (MQ=255)                     gTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCa                                                                                                                           <  1:249552/148‑1 (MQ=255)                      tAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAg                                                                                                                          <  1:223798/148‑1 (MQ=255)                      tAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAg                                                                                                                          <  2:152270/148‑1 (MQ=255)                        aCCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTgg                                                                                                                       >  3:199635/1‑149 (MQ=255)                        aCCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTgg                                                                                                                       <  6:219620/149‑1 (MQ=255)                          cGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCACTGGCa                                                                                                                     >  4:107982/1‑149 (MQ=255)                                   ccAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTAcagg                                                                                                            <  8:265706/149‑1 (MQ=255)                                    cAGAACGCAGACAAAGCCGCCGTTCATGTATTCACAGGAGTTATCGCCAGTGCCCTCGACAACAGGATCGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTAcagg                                                                                                            <  3:187529/148‑1 (MQ=255)                                        aCGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcgag                                                                                                                                       >  5:98185/1‑112 (MQ=255)                                        aCGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGGTACTGCgggcgag                                                                                                                                       <  6:98185/117‑6 (MQ=255)                                          gCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGgtgt                                                                                                     >  4:135198/1‑149 (MQ=255)                                           cAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGACAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCTCAGTAACTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACATGCAGGTGtt                                                                                                    <  6:75390/149‑1 (MQ=255)                                                    ccgccgGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGAt                                                                                           >  4:84304/1‑149 (MQ=255)                                                      gccgGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATgg                                                                                         >  4:283952/1‑149 (MQ=255)                                                         ggTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGgcgc                                                                                      >  2:195870/1‑149 (MQ=255)                                                              tGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGc                                                                                 <  1:195870/149‑1 (MQ=255)                                                                     acaGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGtt                                                                                                    <  6:183858/123‑1 (MQ=255)                                                                     acaGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGtt                                                                                                    >  5:183858/1‑123 (MQ=255)                                                                     acaGCAGTGATCGCCAGTGCCCTCGACAACAGAATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCAGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGttt                                                                          >  5:229389/1‑149 (MQ=255)                                                                         cagTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAg                                                                                                                                     >  8:102805/1‑86 (MQ=255)                                                                         cagTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAg                                                                                                                                     <  7:102805/86‑1 (MQ=255)                                                                           gTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACg                                                                    <  1:142911/149‑1 (MQ=255)                                                                             gATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTgc                                                                                                                                >  5:72767/1‑87 (MQ=255)                                                                             gATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTgc                                                                                                                                <  6:72767/87‑1 (MQ=255)                                                                              aTCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACggg                                                                  <  7:284703/148‑1 (MQ=255)                                                                              aTCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGc                                                                 >  3:244498/1‑149 (MQ=255)                                                                                  ccAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGcc                                                             >  2:200923/1‑149 (MQ=255)                                                                                      tGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCt                                                         <  3:107982/149‑1 (MQ=255)                                                                                         ccTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAATCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTggc                                                      >  7:71953/1‑149 (MQ=255)                                                                                         ccTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTggc                                                      >  2:273952/1‑149 (MQ=255)                                                                                           tCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCt                                                                                     <  4:30955/116‑1 (MQ=255)                                                                                           tCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCt                                                                                     >  3:30955/1‑116 (MQ=255)                                                                                           tCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTggcgg                                                    >  5:13360/1‑149 (MQ=255)                                                                                                aacaGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACggg                                                                  >  6:96238/1‑130 (MQ=255)                                                                                                aacaGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACggg                                                                  <  5:96238/130‑1 (MQ=255)                                                                                                  caGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATg                                             <  3:135198/149‑1 (MQ=255)                                                                                                  caGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATg                                             <  1:273952/149‑1 (MQ=255)                                                                                                                  gTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCgg                             <  3:283952/149‑1 (MQ=255)                                                                                                                     aCGAACAGCGAAGCG‑CTCGCCCGCAGTAACTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGCCAGATGTTGACGACGATGGCGCTGAGCTAGGTTTCGAACGCGCTGCCGGCAGGCGGCACGAAGGTGACCTTGCCACCGCTCa                          >  6:308325/1‑149 (MQ=255)                                                                                                                      cGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCAt                         <  4:199635/149‑1 (MQ=255)                                                                                                                         aCAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTg                                          <  6:237961/129‑1 (MQ=255)                                                                                                                         aCAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTg                                          >  5:237961/1‑129 (MQ=255)                                                                                                                          cAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCt                     >  4:290196/1‑149 (MQ=255)                                                                                                                          cAGCGAAGAG‑AGCGCCCGCAGTACCTGCGGCAAACAGCGTGCAGACAGTGTAACCTTACAGGAAGGTTTTGCCTACTATGGCGCTGTGCTGTGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGAACTTGCCACCTGTCATGcc                      <  2:54735/148‑1 (MQ=255)                                                                                                                           aGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCtt                    >  3:294594/1‑149 (MQ=255)                                                                                                                           aGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCtt                    >  1:146311/1‑149 (MQ=255)                                                                                                                           aGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCtt                    >  2:325538/1‑149 (MQ=255)                                                                                                                            gCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCttt                   >  5:103021/1‑149 (MQ=255)                                                                                                                            gCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCttt                   >  1:229118/1‑149 (MQ=255)                                                                                                                              gAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGc                 <  6:103021/149‑1 (MQ=255)                                                                                                                                   g‑cTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGc                       >  7:155998/1‑138 (MQ=255)                                                                                                                                   g‑cTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGc                       <  8:155998/138‑1 (MQ=255)                                                                                                                                   g‑cTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACg            >  6:272658/1‑149 (MQ=255)                                                                                                                                     cTCGCACGCACTACCTGCGCGAAACACCTTGCCGCCAGTGGCACCGTACAGGGAGCTCTTCCCGCCGCTGCCGCTGTGCTGGCTTTCGCAAGGGCTGACTGCTCCCCCCACGATGGTGGCATTGCCACCGGTAATGCCTTTGCCGACg            >  2:310691/1‑148 (MQ=255)                                                                                                                                      tCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGt           >  5:64048/1‑148 (MQ=255)                                                                                                                                             cAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTg   <  1:200923/149‑1 (MQ=255)                                                                                                                                              aGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTgg  <  1:270396/149‑1 (MQ=255)                                                                                                                                              aGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTg   >  4:49951/1‑148 (MQ=255)                                                                                                                                              aGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTg   <  3:49951/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GTCATGCCAGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCG‑CTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGG  >  NC_002947/5793731‑5794019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑