Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,819,408	G→T	6.3%	R226L (CGC→CTC)	proI →	pyrroline‑5‑carboxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,819,408	0	G	T	6.3%	202.7 / 3.2	63	R226L (CGC→CTC)	proI	pyrroline‑5‑carboxylate reductase
Reads supporting (aligned to +/- strand):  ref base G (27/32);  new base T (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GCGTACTTCTTCCTGCTGATCGAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACTGGGCAAATAAG  >  NC_002947/5819260‑5819551                                                                                                                                                      |                                                                                                                                                gCGTACTTCTTCCTGCTGATCGAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTgcg                                                                                                                                                 <  4:290533/149‑1 (MQ=255)  cGTACTTCTTCCTGCTGATCGAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTgcgc                                                                                                                                                <  6:3994/149‑1 (MQ=255)       ttcttcCTGCTGATCGAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGcgccgccg                                                                                                                                           >  4:72415/1‑149 (MQ=255)          ttcCTGCTGATCGAGGCCATGACCGCGGTAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGt                                                                                                                                        >  1:168272/1‑149 (MQ=255)              tgctgATCGAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACt                                                                                                                                    >  5:241530/1‑149 (MQ=255)                ctgATCGAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTc                                                                                                                                  >  6:96230/1‑149 (MQ=255)                    tCGAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTcgccc                                                                                                                              <  5:302727/149‑1 (MQ=255)                      gAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTc                                                                                                                                  >  3:102535/1‑143 (MQ=255)                      gAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTc                                                                                                                                  <  4:102535/143‑1 (MQ=255)                       aGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTcgcccgcc                                                                                                                           <  2:212240/149‑1 (MQ=255)                             tGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGcac                                                                                                                      >  5:310646/1‑148 (MQ=255)                                ccGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACg                                                                                                                  <  4:199780/149‑1 (MQ=255)                                            aaaaaCTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATc                                                                                                      <  6:242681/149‑1 (MQ=255)                                            aaaaaCTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATc                                                                                                      <  7:102631/149‑1 (MQ=255)                                            aaaaaCTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATc                                                                                                      <  5:96230/149‑1 (MQ=255)                                               aaCTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAg                                                                                                   <  3:317811/149‑1 (MQ=255)                                                      ccTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGGAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTcc                                                                                            >  6:264640/1‑149 (MQ=255)                                                      ccTGCCACGCGAAACGGACTCCCAACTGACCTTGCAGACTGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCCC‑TGAACTGCGCGGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTcc                                                                                            <  6:198470/149‑1 (MQ=255)                                                          ccACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGc                                                                                        >  8:122734/1‑149 (MQ=255)                                                          ccACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGc                                                                                        >  8:241395/1‑149 (MQ=255)                                                                         tCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGc                                                                         >  5:272066/1‑149 (MQ=255)                                                                           ccAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCa                                                                       >  6:105825/1‑149 (MQ=255)                                                                                  aCCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCAtcgtc                                                                  <  6:241530/147‑1 (MQ=255)                                                                                  aCCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGa                                                                >  3:302872/1‑149 (MQ=255)                                                                                   ccTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAg                                                               <  7:241395/149‑1 (MQ=255)                                                                                    cTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAg                                                               >  4:151127/1‑148 (MQ=255)                                                                                       gCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCTCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAgg                                                           >  4:35077/1‑149 (MQ=255)                                                                                       gCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCTCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAgg                                                           >  4:39184/1‑149 (MQ=255)                                                                                       gCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAgg                                                           >  7:31332/1‑149 (MQ=255)                                                                                               cccTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGCGTGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCgctg                                                      >  1:72074/1‑147 (MQ=255)                                                                                               cccTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCgctgcag                                                   >  5:161379/1‑149 (MQ=255)                                                                                               cccTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCgctgcag                                                   >  3:19329/1‑149 (MQ=255)                                                                                                ccTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCgctgcagg                                                  <  3:72415/149‑1 (MQ=255)                                                                                                  tGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGc                                                              >  4:230693/1‑135 (MQ=255)                                                                                                  tGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGc                                                              <  3:230693/135‑1 (MQ=255)                                                                                                      cgccgcGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGTCACCACGGAAGCGGCGATCAAGTCGTTCCAg                                                                                          <  8:157316/103‑1 (MQ=255)                                                                                                               cATGGTAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGct                                   <  5:19293/149‑1 (MQ=255)                                                                                                               cATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAgg                                                           <  2:275905/125‑1 (MQ=255)                                                                                                                 tGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGctct                                 >  4:13877/1‑149 (MQ=255)                                                                                                                  ggCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCAcgcg                                     >  1:254707/1‑144 (MQ=255)                                                                                                                  ggCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCAcgcg                                     <  2:254707/144‑1 (MQ=255)                                                                                                                       ttGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAg                           >  6:251266/1‑149 (MQ=255)                                                                                                                       ttGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAg                           >  4:305212/1‑149 (MQ=255)                                                                                                                          ctaGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTGGCCCGCCGGCACCACGGATGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTg                        <  4:221672/147‑1 (MQ=255)                                                                                                                          cAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTg                        <  7:80329/149‑1 (MQ=255)                                                                                                                           aaGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGTCGATCAAGTCGTTCCAGGCCAGCGGCTTCGaa                                                                           <  1:272872/97‑1 (MQ=255)                                                                                                                           aaGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGaa                                                                           >  2:272872/1‑97 (MQ=255)                                                                                                                           aaGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTgg                       <  6:272066/149‑1 (MQ=255)                                                                                                                            aGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGc                      >  5:317536/1‑149 (MQ=255)                                                                                                                            aGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGc                      <  5:251266/149‑1 (MQ=255)                                                                                                                                aTGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGaa                  <  6:161379/149‑1 (MQ=255)                                                                                                                                 tGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGaac                 <  5:264640/149‑1 (MQ=255)                                                                                                                                  gTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCgctgcaggctgc                                              >  5:272559/1‑119 (MQ=255)                                                                                                                                  gTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCgctgcaggctgc                                              <  6:272559/119‑1 (MQ=255)                                                                                                                                  gTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTCCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTg                                >  1:325245/1‑133 (MQ=255)                                                                                                                                  gTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTCCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTg                                <  2:325245/133‑1 (MQ=255)                                                                                                                                     gATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACt             <  8:31332/149‑1 (MQ=255)                                                                                                                                      aTGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACTg            <  5:105825/149‑1 (MQ=255)                                                                                                                                         ccGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACTggg          <  3:305212/148‑1 (MQ=255)                                                                                                                                          cGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACTGGGc         >  7:269943/1‑148 (MQ=255)                                                                                                                                                aaCTGCTCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACTTGGCAAATaa   <  3:39184/149‑1 (MQ=255)                                                                                                                                                aaCTGCTCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACTGGGCAAATaa   <  3:35077/149‑1 (MQ=255)                                                                                                                                                aaCTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACTGGGCAAATaa   <  6:107409/149‑1 (MQ=255)                                                                                                                                                 aCTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACTGGGCAAATAAg  <  3:13877/149‑1 (MQ=255)                                                                                                                                                   tGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGaa                  >  7:293703/1‑131 (MQ=255)                                                                                                                                                   tGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGaa                  <  8:293703/131‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                GCGTACTTCTTCCTGCTGATCGAGGCCATGACCGCGGCAGGCGAAAAACTCGGCCTGCCACGCGAAACGGCCTCCCAACTGACCTTGCAGACAGCCCTGGGCGCCGCGCGCATGGCAGTTGCAAGCGATGTCGATGCCGC‑TGAACTGCGCCGCCGCGTCACTTCGCCCGCCGGCACCACGGAAGCGGCGATCAAGTCGTTCCAGGCCAGCGGCTTCGAAGCCATCGTCGAGCAGGCGCTGCAGGCTGCAGCCACGCGCTCTGCCGAGCTGGCCGAACAACTGGGCAAATAAG  >  NC_002947/5819260‑5819551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑