Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,857,702 A→C 6.7% E84A (GAG→GCG) ‡ PP_5134 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,857,7020AC 6.7% 223.7 / 7.0 75E84A (GAG→GCG) ‡PP_5134hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (34/36);  new base C (2/3);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

GTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGT  >  NC_002947/5857554‑5857843
                                                                                                                                                    |                                                                                                                                              
gtgtCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGAAGCGCCCAGGCGa                                                                                                                                                <  3:298139/149‑1 (MQ=255)
   tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAgcg                                                                                                                                             >  2:49821/1‑149 (MQ=255)
     ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGCTCGcc                                                                                                                                           >  3:242510/1‑149 (MQ=255)
     ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGcc                                                                                                                                           <  6:325316/149‑1 (MQ=255)
     ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGcc                                                                                                                                           >  8:128566/1‑149 (MQ=255)
      cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCt                                                                                                                                          >  8:95126/1‑149 (MQ=255)
      cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCt                                                                                                                                          >  4:203600/1‑149 (MQ=255)
       aCCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTg                                                                                                                                         >  2:253862/1‑149 (MQ=255)
        ccGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTgg                                                                                                                                        <  7:270879/149‑1 (MQ=255)
         cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGCTCGCCTggg                                                                                                                                       <  1:8427/149‑2 (MQ=255)
         cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGCTCGCCTggg                                                                                                                                       <  4:242510/149‑2 (MQ=255)
          gCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGCTCGCCTgggc                                                                                                                                      <  3:181401/149‑3 (MQ=255)
            cccAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACgg                                                                                                                                    <  1:235523/149‑1 (MQ=255)
             ccAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGc                                                                                                                                   >  2:134118/1‑149 (MQ=255)
              cAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGcc                                                                                                                                  >  8:120863/1‑149 (MQ=255)
                gCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCt                                                                                                                                <  8:27015/149‑1 (MQ=255)
                  actactCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGc                                                                                                                              <  6:201438/149‑1 (MQ=255)
                       tCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGc                                                                                                                         >  6:121692/1‑149 (MQ=255)
                         gCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTg                                                                                                                       >  2:1554/1‑149 (MQ=255)
                          ccATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGCCGGCCCTGCCCGGCTgg                                                                                                                      >  3:257619/1‑149 (MQ=255)
                           cATGTGGAAGGTTCACTGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACATGCCCGTCCTGGAAGATTCCATCGCGCTGGTCGACTACCTTGAACGCCTGGAGCGCGCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                                        <  4:234184/146‑1 (MQ=255)
                           cATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGTCCCTGCCCGGCTg                                                                                                                       >  7:242111/1‑147 (MQ=255)
                           cATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                                     <  1:49821/149‑1 (MQ=255)
                              gtgGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCg                                                                                                                  >  5:81045/1‑149 (MQ=255)
                                ggAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGt‑t                                                                                                               <  7:120863/149‑1 (MQ=255)
                                 gAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGt‑tt                                                                                                              <  5:121692/149‑1 (MQ=255)
                                  aaGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGt‑ttt                                                                                                             >  5:101075/1‑149 (MQ=255)
                                    ggTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCt                                                                                                           <  2:73529/149‑1 (MQ=255)
                                      tGCAGGGATGTCGGTGTAATGTGAAAATTTGCTGTAGCTGTACGACACGCCCGGCATGGAATATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCACAGGCGAGCGCCTGGACGTACCTGCCATGCTGGAGAGT‑TTTccg                                                                                                          <  8:242111/148‑3 (MQ=255)
                                        cACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCa                                                                                                        <  2:25070/148‑1 (MQ=255)
                                        cACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAg                                                                                                       >  5:16467/1‑149 (MQ=255)
                                          cGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAggg                                                                                                     >  6:160097/1‑149 (MQ=255)
                                          cGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAggg                                                                                                     <  6:101075/149‑1 (MQ=255)
                                                       aCGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAgcgcgc                                                                                        <  1:253862/149‑1 (MQ=255)
                                                         ggTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGcc                                                                                       >  6:108618/1‑148 (MQ=255)
                                                            gAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACg                                                                                   >  8:147720/1‑149 (MQ=255)
                                                                   tGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGa                                                                             <  3:190698/148‑1 (MQ=255)
                                                                   tGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGa                                                                             <  1:134118/148‑1 (MQ=255)
                                                                    gCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                            <  5:23944/148‑1 (MQ=255)
                                                                     cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGcc                                                                                                                                  >  7:217479/1‑94 (MQ=255)
                                                                     cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGcc                                                                                                                                  <  8:217479/94‑1 (MQ=255)
                                                                     cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                            >  4:135803/1‑147 (MQ=255)
                                                                     cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGc                                                                           <  6:81045/148‑1 (MQ=255)
                                                                     cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGc                                                                           <  7:128566/148‑1 (MQ=255)
                                                                      tGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCag                                                                         >  3:275514/1‑149 (MQ=255)
                                                                        gAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTg                                                                                                                       >  4:303463/1‑102 (MQ=255)
                                                                         aGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                                      <  5:160097/149‑1 (MQ=255)
                                                                         aGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                                      <  7:95126/149‑1 (MQ=255)
                                                                          gCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggagg                                                                     <  8:300260/149‑1 (MQ=255)
                                                                             gTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGcc                                                                   >  2:264091/1‑148 (MQ=255)
                                                                              tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCa                                                                          <  1:50678/140‑1 (MQ=255)
                                                                              tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCa                                                                          >  2:50678/1‑140 (MQ=255)
                                                                                 gACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAgg                                                               >  6:102241/1‑148 (MQ=255)
                                                                                   cacGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAt                                                            <  6:16467/149‑1 (MQ=255)
                                                                                      gCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgc                                                         <  5:108618/149‑1 (MQ=255)
                                                                                        cctgCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                       <  3:203600/146‑1 (MQ=255)
                                                                                        ccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                       <  4:275514/149‑1 (MQ=255)
                                                                                               ggAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgc                                                >  4:74168/1‑149 (MQ=255)
                                                                                                     tGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCa                                          <  2:122183/149‑1 (MQ=255)
                                                                                                              ccTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCg                                 >  1:209985/1‑149 (MQ=255)
                                                                                                                 gCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGcc                              >  8:180518/1‑149 (MQ=255)
                                                                                                                  cTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                               <  1:264091/147‑1 (MQ=255)
                                                                                                                    cGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTg                            >  3:186288/1‑148 (MQ=255)
                                                                                                                      aCTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAt                         <  7:147720/149‑1 (MQ=255)
                                                                                                                          ccTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGTAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgt                       <  7:72710/147‑1 (MQ=255)
                                                                                                                          ccTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgtg                      <  3:135803/148‑1 (MQ=255)
                                                                                                                           cTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt                    >  2:262775/1‑149 (MQ=255)
                                                                                                                           cTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt                    <  5:102241/149‑1 (MQ=255)
                                                                                                                           cTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt                    >  8:292606/1‑149 (MQ=255)
                                                                                                                              gAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTATTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                   >  1:248975/1‑148 (MQ=255)
                                                                                                                                aCGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATg               >  1:245806/1‑149 (MQ=255)
                                                                                                                                aCGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGACCAGTCATGCCGGCCTGTATGTGATCGATg               >  7:22192/1‑149 (MQ=255)
                                                                                                                                   ccTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcgc            <  4:257619/149‑1 (MQ=255)
                                                                                                                                   ccTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcgc            <  1:262775/149‑1 (MQ=255)
                                                                                                                                           gtccAGGCGCTCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCACGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCg    >  6:42817/3‑149 (MQ=255)
                                                                                                                                             ccAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGt  >  3:172855/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGT  >  NC_002947/5857554‑5857843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑