Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,857,703 G→T 6.7% E84D (GAG→GAT) ‡ PP_5134 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,857,7030GT 6.7% 238.8 / 4.6 74E84D (GAG→GAT) ‡PP_5134hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/36);  new base T (2/3);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

TCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGT  >  NC_002947/5857557‑5857843
                                                                                                                                                  |                                                                                                                                             
tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAgcg                                                                                                                                             >  2:49821/1‑149 (MQ=255)
  ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGCTCGcc                                                                                                                                           >  3:242510/1‑149 (MQ=255)
  ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGcc                                                                                                                                           <  6:325316/149‑1 (MQ=255)
  ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGcc                                                                                                                                           >  8:128566/1‑149 (MQ=255)
   cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCt                                                                                                                                          >  8:95126/1‑149 (MQ=255)
   cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCt                                                                                                                                          >  4:203600/1‑149 (MQ=255)
    aCCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTg                                                                                                                                         >  2:253862/1‑149 (MQ=255)
     ccGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTgg                                                                                                                                        <  7:270879/149‑1 (MQ=255)
      cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGCTCGCCTggg                                                                                                                                       <  4:242510/149‑2 (MQ=255)
      cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGCTCGCCTggg                                                                                                                                       <  1:8427/149‑2 (MQ=255)
       gCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGCTCGCCTgggc                                                                                                                                      <  3:181401/149‑3 (MQ=255)
         cccAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACgg                                                                                                                                    <  1:235523/149‑1 (MQ=255)
          ccAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGc                                                                                                                                   >  2:134118/1‑149 (MQ=255)
           cAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGcc                                                                                                                                  >  8:120863/1‑149 (MQ=255)
             gCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCt                                                                                                                                <  8:27015/149‑1 (MQ=255)
               actactCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGc                                                                                                                              <  6:201438/149‑1 (MQ=255)
                    tCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGc                                                                                                                         >  6:121692/1‑149 (MQ=255)
                      gCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTg                                                                                                                       >  2:1554/1‑149 (MQ=255)
                       ccATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGCCGGCCCTGCCCGGCTgg                                                                                                                      >  3:257619/1‑149 (MQ=255)
                        cATGTGGAAGGTTCACTGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACATGCCCGTCCTGGAAGATTCCATCGCGCTGGTCGACTACCTTGAACGCCTGGAGCGCGCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                                        <  4:234184/146‑1 (MQ=255)
                        cATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGTCCCTGCCCGGCTg                                                                                                                       >  7:242111/1‑147 (MQ=255)
                        cATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                                     <  1:49821/149‑1 (MQ=255)
                           gtgGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCg                                                                                                                  >  5:81045/1‑149 (MQ=255)
                             ggAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGt‑t                                                                                                               <  7:120863/149‑1 (MQ=255)
                              gAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGt‑tt                                                                                                              <  5:121692/149‑1 (MQ=255)
                               aaGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGt‑ttt                                                                                                             >  5:101075/1‑149 (MQ=255)
                                 ggTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCt                                                                                                           <  2:73529/149‑1 (MQ=255)
                                   tGCAGGGATGTCGGTGTAATGTGAAAATTTGCTGTAGCTGTACGACACGCCCGGCATGGAATATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCACAGGCGAGCGCCTGGACGTACCTGCCATGCTGGAGAGT‑TTTccg                                                                                                          <  8:242111/148‑3 (MQ=255)
                                     cACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCa                                                                                                        <  2:25070/148‑1 (MQ=255)
                                     cACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAg                                                                                                       >  5:16467/1‑149 (MQ=255)
                                       cGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAggg                                                                                                     <  6:101075/149‑1 (MQ=255)
                                       cGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAggg                                                                                                     >  6:160097/1‑149 (MQ=255)
                                                    aCGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAgcgcgc                                                                                        <  1:253862/149‑1 (MQ=255)
                                                      ggTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGcc                                                                                       >  6:108618/1‑148 (MQ=255)
                                                         gAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACg                                                                                   >  8:147720/1‑149 (MQ=255)
                                                                tGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGa                                                                             <  3:190698/148‑1 (MQ=255)
                                                                tGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGa                                                                             <  1:134118/148‑1 (MQ=255)
                                                                 gCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                            <  5:23944/148‑1 (MQ=255)
                                                                  cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGcc                                                                                                                                  <  8:217479/94‑1 (MQ=255)
                                                                  cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGcc                                                                                                                                  >  7:217479/1‑94 (MQ=255)
                                                                  cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                            >  4:135803/1‑147 (MQ=255)
                                                                  cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGc                                                                           <  7:128566/148‑1 (MQ=255)
                                                                  cTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGc                                                                           <  6:81045/148‑1 (MQ=255)
                                                                   tGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCag                                                                         >  3:275514/1‑149 (MQ=255)
                                                                     gAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTg                                                                                                                       >  4:303463/1‑102 (MQ=255)
                                                                      aGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                                      <  7:95126/149‑1 (MQ=255)
                                                                      aGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                                      <  5:160097/149‑1 (MQ=255)
                                                                       gCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggagg                                                                     <  8:300260/149‑1 (MQ=255)
                                                                          gTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGcc                                                                   >  2:264091/1‑148 (MQ=255)
                                                                           tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCa                                                                          >  2:50678/1‑140 (MQ=255)
                                                                           tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCa                                                                          <  1:50678/140‑1 (MQ=255)
                                                                              gACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAgg                                                               >  6:102241/1‑148 (MQ=255)
                                                                                cacGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAt                                                            <  6:16467/149‑1 (MQ=255)
                                                                                   gCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgc                                                         <  5:108618/149‑1 (MQ=255)
                                                                                     cctgCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                       <  3:203600/146‑1 (MQ=255)
                                                                                     ccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                       <  4:275514/149‑1 (MQ=255)
                                                                                            ggAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgc                                                >  4:74168/1‑149 (MQ=255)
                                                                                                  tGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCa                                          <  2:122183/149‑1 (MQ=255)
                                                                                                           ccTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCg                                 >  1:209985/1‑149 (MQ=255)
                                                                                                              gCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGcc                              >  8:180518/1‑149 (MQ=255)
                                                                                                               cTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                               <  1:264091/147‑1 (MQ=255)
                                                                                                                 cGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTg                            >  3:186288/1‑148 (MQ=255)
                                                                                                                   aCTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAt                         <  7:147720/149‑1 (MQ=255)
                                                                                                                       ccTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGTAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgt                       <  7:72710/147‑1 (MQ=255)
                                                                                                                       ccTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgtg                      <  3:135803/148‑1 (MQ=255)
                                                                                                                        cTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt                    >  2:262775/1‑149 (MQ=255)
                                                                                                                        cTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt                    <  5:102241/149‑1 (MQ=255)
                                                                                                                        cTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt                    >  8:292606/1‑149 (MQ=255)
                                                                                                                           gAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTATTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                   >  1:248975/1‑148 (MQ=255)
                                                                                                                             aCGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATg               >  1:245806/1‑149 (MQ=255)
                                                                                                                             aCGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGACCAGTCATGCCGGCCTGTATGTGATCGATg               >  7:22192/1‑149 (MQ=255)
                                                                                                                                ccTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcgc            <  4:257619/149‑1 (MQ=255)
                                                                                                                                ccTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcgc            <  1:262775/149‑1 (MQ=255)
                                                                                                                                        gtccAGGCGCTCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCACGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCg    >  6:42817/3‑149 (MQ=255)
                                                                                                                                          ccAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGt  >  3:172855/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
TCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGT‑TTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGT  >  NC_002947/5857557‑5857843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑