Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,866,697	A→C	9.8%	intergenic (+20/+37)	PP_5140 → / ← thyA	MerR family transcriptional regulator/thymidylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,866,697	0	A	C	9.8%	174.8 / 9.6	61	intergenic (+20/+37)	PP_5140/thyA	MerR family transcriptional regulator/thymidylate synthase
Reads supporting (aligned to +/- strand):  ref base A (27/28);  new base C (2/4);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTCGG  >  NC_002947/5866557‑5866843                                                                                                                                             |                                                                                                                                                    gcggcggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGa                                                                                                                                             <  4:24816/149‑1 (MQ=255)  cggcggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGaa                                                                                                                                            <  7:294095/149‑1 (MQ=255)     cggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAagag                                                                                                                                         <  4:199853/149‑1 (MQ=255)     cggcgCTGCAACGCGCAGGGGAGGGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAagag                                                                                                                                         <  5:155044/149‑1 (MQ=255)          cTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGAagcttttgg                                                                                                                                    <  5:243710/149‑8 (MQ=255)          cTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAg                                                                                                                                    >  6:198455/1‑149 (MQ=255)          cTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAg                                                                                                                                    <  4:160404/149‑1 (MQ=255)                 gcgcAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTaa                                                                                                                              >  8:177074/1‑148 (MQ=255)                 gcgcAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTaa                                                                                                                              >  4:61441/1‑148 (MQ=255)                               aTGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCTGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTAt                                                                                                                <  8:246743/148‑1 (MQ=255)                                  cgcgATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGa                                                                                                            >  6:105180/1‑149 (MQ=255)                                   gcgATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGAc                                                                                                           >  3:317808/1‑149 (MQ=255)                                   gcgATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGAc                                                                                                           >  2:48673/1‑149 (MQ=255)                                             agcaACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCcgc                                                                                                                                               <  4:120364/103‑1 (MQ=255)                                             agcaACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCcgc                                                                                                                                               >  3:120364/1‑103 (MQ=255)                                             agcaACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGgc                                                                                                 <  3:61441/149‑1 (MQ=255)                                              gcaACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGgcg                                                                                                >  4:148491/1‑149 (MQ=255)                                              gcaACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGgcg                                                                                                <  5:105180/149‑1 (MQ=255)                                              gcaACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGgcg                                                                                                <  7:177074/149‑1 (MQ=255)                                                      agaCAAACGGGGCGGTCTCGGTACCGGATACCGCACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAACGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATc                                                                                        <  4:5423/149‑1 (MQ=255)                                                          aaaCGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGgcg                                                                                    >  1:140727/1‑149 (MQ=255)                                                              ggggCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATg                                                                                <  2:184384/149‑1 (MQ=255)                                                                    gTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGTGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCt                                                                           >  2:201232/1‑148 (MQ=255)                                                                       tCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATa                                                                       <  5:198455/149‑1 (MQ=255)                                                                       tCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATa                                                                       <  5:78456/149‑1 (MQ=255)                                                                               ggATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTcca                                                               <  5:313249/149‑1 (MQ=255)                                                                                 aTACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagc                                                             >  5:291710/1‑149 (MQ=255)                                                                                    ccGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGTGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagcca                                                           <  1:201232/148‑1 (MQ=255)                                                                                     cGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagccag                                                          >  6:106344/1‑148 (MQ=255)                                                                                     cGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagccag                                                          >  1:161985/1‑148 (MQ=255)                                                                                         taTTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATc                                                     <  5:43710/148‑1 (MQ=255)                                                                                         taTTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATc                                                     <  5:39039/148‑1 (MQ=255)                                                                                          aTTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCa                                                    <  4:317808/149‑1 (MQ=255)                                                                                          aTTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCa                                                    <  2:140727/149‑1 (MQ=255)                                                                                              ccATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑Cg                                               >  6:285870/1‑149 (MQ=255)                                                                                                aTGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGc                                             >  8:229023/1‑149 (MQ=255)                                                                                                  gtgGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCt                                            >  7:166420/1‑148 (MQ=255)                                                                                                   tgGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCa                                          <  5:308984/149‑1 (MQ=255)                                                                                                     gggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCaa                                         <  3:148491/148‑1 (MQ=255)                                                                                                              cACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAg                               >  1:94060/1‑149 (MQ=255)                                                                                                               aCGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGc                              >  3:21519/1‑149 (MQ=255)                                                                                                               aCGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCGGTCATCGGCGCATGCTCCTCATAGCCTTCCAGCCAGAAATCACT‑AGGCTCAATCTTCTCCAGc                              >  7:79140/1‑149 (MQ=255)                                                                                                                   gCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagcca                                                           >  5:159191/1‑117 (MQ=255)                                                                                                                   gCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagcca                                                           <  6:159191/117‑1 (MQ=255)                                                                                                                   gCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACt                          >  5:132264/1‑149 (MQ=255)                                                                                                                        gAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGgcg                                                                                    >  6:30765/1‑87 (MQ=255)                                                                                                                         aGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGc                     <  2:161985/148‑1 (MQ=255)                                                                                                                            cAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGATATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCTCa                  >  1:70310/1‑148 (MQ=255)                                                                                                                                ctcTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTtctc                                  >  3:32644/3‑128 (MQ=255)                                                                                                                                ctcTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTtctc                                  <  4:32644/126‑1 (MQ=255)                                                                                                                                aGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTACGGCTCAATCTTCTCCAGCCACTCCGGCTCATACt              <  1:48673/149‑1 (MQ=255)                                                                                                                                aGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCTCATACtt             <  2:94060/149‑1 (MQ=255)                                                                                                                                 gCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCt                    >  1:316795/1‑141 (MQ=255)                                                                                                                                 gCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCt                    <  2:316795/141‑1 (MQ=255)                                                                                                                                   ttCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGc           >  2:163167/1‑148 (MQ=255)                                                                                                                                     cgcgGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCa         <  6:291710/148‑1 (MQ=255)                                                                                                                                     cgcgGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCa         <  8:166420/148‑1 (MQ=255)                                                                                                                                      gcgGGTTCACCCGCGAAGCGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCa                                >  3:187555/1‑124 (MQ=255)                                                                                                                                      gcgGGTTCACCCGCGAAGCGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCa                                <  4:187555/124‑1 (MQ=255)                                                                                                                                       cgGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGtt      >  7:205763/1‑149 (MQ=255)                                                                                                                                           tGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTCgg  >  4:184239/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                    GCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACT‑CGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTCGG  >  NC_002947/5866557‑5866843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑