Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,948,346 G→T 100% A43E (GCG→GAG)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,948,3460GT96.7% 198.0 / ‑1.1 61A43E (GCG→GAG) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (30/29);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGTTCATAAGGGGAA  >  NC_002947/5948201‑5948481
                                                                                                                                                 |                                                                                                                                       
cAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCAt                                                                                                                                      >  7:136741/1‑149 (MQ=255)
 aGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATg                                                                                                                                     <  4:89505/149‑1 (MQ=255)
  ggTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGc                                                                                                                                    <  3:73390/149‑1 (MQ=255)
                     gcttgGCGAGACATAGATTTCGTGTGGGCCGGCCTGGTAGGACGCATCAGCCGAACGCAGGAATCCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCAt                                                                                                                                      <  6:56550/128‑1 (MQ=255)
                     gctggGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCATGGAGAATAACCAGCACGCCGTCACCCGAGATCTCTTCGCAGCTTATCTCAt                                                                                                                                      >  5:56550/1‑128 (MQ=255)
                     gctggGCGAGACATAGATATCGGCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCg                                                                                                                     <  5:285234/145‑1 (MQ=255)
                         ggCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATc                                                                                                               <  7:2125/147‑1 (MQ=255)
                         ggCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCAc                                                                                                             <  4:112573/149‑1 (MQ=255)
                              gaCATACATATCCTCGGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCCTCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCt                                                                                                        <  3:143133/149‑1 (MQ=255)
                                 aTAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGtt                                                                                                     >  1:148167/1‑149 (MQ=255)
                                 aTAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGtt                                                                                                     >  8:274832/1‑149 (MQ=255)
                                 aTAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGtt                                                                                                     >  3:12531/1‑149 (MQ=255)
                                  tAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGttt                                                                                                    <  2:148167/149‑1 (MQ=255)
                                          gTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAAc                                                                                            >  5:166351/1‑149 (MQ=255)
                                          gTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAAc                                                                                            >  6:68404/1‑149 (MQ=255)
                                          gTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAAc                                                                                            >  7:210896/1‑149 (MQ=255)
                                          gTCTGGGCCGGCCAGATAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAAc                                                                                            >  2:11635/1‑149 (MQ=255)
                                           tCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCATCGCCGCTTTTCTCATGCTTCTTCAGCACGGCGAAAATCACGTCCTGTTTGCGCGAACg                                                                                           >  3:21256/1‑149 (MQ=255)
                                               ggccggccAGTTATGACGCATCAGCCGAACGCATGAAACCGATAGCATCCTGGAGATTCTCCTGCACCGCGTCACCCGAGATGTCTTCGCCGTTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACgg                                                                                          <  3:18669/146‑1 (MQ=255)
                                                      cAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGACAATCACGTCCTGTTTGCGCGAACGGGCCATGtttt                                                                                >  5:100094/1‑149 (MQ=255)
                                                      cAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAgg                                                                                                                        <  3:282670/109‑1 (MQ=255)
                                                      cAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAgg                                                                                                                        >  4:282670/1‑109 (MQ=255)
                                                       aGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTc                                                                               <  6:77935/149‑1 (MQ=255)
                                                         gTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGa                                                                             <  8:73698/149‑1 (MQ=255)
                                                            ggACGCATCAGCCGAACGCAGGAAACCGAAACCATACTGGAGAATCTCCAGCACGCCGTGACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATg                                                                           <  5:307251/148‑1 (MQ=255)
                                                              aCGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGccc                                                                        <  3:227382/149‑1 (MQ=255)
                                                                gCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCAt                                                                      <  7:274832/149‑1 (MQ=255)
                                                                gCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCAt                                                                      >  7:140137/1‑149 (MQ=255)
                                                                  aTCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCt                                                                    >  6:263429/1‑149 (MQ=255)
                                                                                   aaccgaaaccATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCaaa                                                   <  5:263429/149‑1 (MQ=255)
                                                                                    accgaaaccATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCaaaa                                                  <  6:100094/149‑1 (MQ=255)
                                                                                     ccgaaaccATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGGCATGTTTTCGATGCCAATCTGTTCGGCCATTTCCAAAAg                                                 >  2:197281/1‑149 (MQ=255)
                                                                                      cgaaaccATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTATTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATCTTTTCGATCCCCATCTGTTCGGCCATTTCCaa                                                    >  8:248355/1‑145 (MQ=255)
                                                                                          accATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCgg                                            <  8:23012/149‑1 (MQ=255)
                                                                                              tCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCg                                             >  5:22129/1‑144 (MQ=255)
                                                                                              tCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCg                                             <  6:22129/144‑1 (MQ=255)
                                                                                                cTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCAt                                                                                     >  5:157907/1‑102 (MQ=255)
                                                                                                cTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCAt                                                                                     <  6:157907/102‑1 (MQ=255)
                                                                                                   gagaATCTCCAGCACGCCGTCACCCGATATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCgg                                     <  8:136741/147‑1 (MQ=255)
                                                                                                     gaATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCTAAAAGATCGGTAATCGGCttt                                 >  1:284059/1‑149 (MQ=255)
                                                                                                           ccAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCAATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTg                           >  7:3568/1‑149 (MQ=255)
                                                                                                            cAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATg                                                                           <  6:72783/100‑1 (MQ=255)
                                                                                                            cAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATg                                                                           >  5:72783/1‑100 (MQ=255)
                                                                                                            cAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGa                          <  8:210896/149‑1 (MQ=255)
                                                                                                                aCGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGtt                       >  4:27073/1‑148 (MQ=255)
                                                                                                                aCGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGtt                       >  6:56333/1‑148 (MQ=255)
                                                                                                                aCGCCGTCACCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGtt                       >  5:28507/1‑148 (MQ=255)
                                                                                                                     gTCCCCCGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGAAGCCGATCTGTTCGGCCATATCCAAAAGATCGGTAATCGGCTTTTGCTTGa                          >  7:189715/1‑140 (MQ=255)
                                                                                                                     gTCACCCGTGATCTCTTCGACGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGGTAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCGAAATGATCGGTAATCGGCTTTTGCTTGa                          <  8:189715/140‑1 (MQ=255)
                                                                                                                         cccGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGtt             <  3:27073/149‑1 (MQ=255)
                                                                                                                          ccGAGATCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGtt             >  8:94216/1‑148 (MQ=255)
                                                                                                                            gagaTCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTtca                     >  7:244186/1‑138 (MQ=255)
                                                                                                                            gagaTCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTtca                     <  8:244186/138‑1 (MQ=255)
                                                                                                                              gaTCTCTTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGTTCAt          >  5:276689/1‑147 (MQ=255)
                                                                                                                                   ctTCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGATTTCAGTCAGGTTCATAAgggg    >  5:139707/1‑148 (MQ=255)
                                                                                                                                    ttCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCTAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGTTCATAAggg     <  2:284059/146‑1 (MQ=255)
                                                                                                                                    ttCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGTTCATAAgggg    <  1:11635/147‑1 (MQ=255)
                                                                                                                                    ttCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGTTCATAAggg     <  2:93624/146‑1 (MQ=255)
                                                                                                                                    ttCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGTTCATAAgg      <  6:28507/145‑1 (MQ=255)
                                                                                                                                    ttCGCCGCTTTTCTCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGTTCATAAGGGGaa  >  2:234023/1‑149 (MQ=255)
                                                                                                                                    ttCGCCGCTTTTCTCATGCTTCGTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGTTCATAAGGGGaa  >  7:49551/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                       
CAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGGCCATTTCCAAAAGATCGGTAATCGGCTTTTGCTTGAGTTCAGTCAGGTTCATAAGGGGAA  >  NC_002947/5948201‑5948481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑