Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,035,933 A→G 8.7% I155V (ATC→GTC) ‡ algC → phosphomannomutase/phosphoglucomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,035,9330AG 8.7% 120.6 / 3.0 47I155V (ATC→GTC) ‡algCphosphomannomutase/phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base A (23/19);  new base G (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

GGCAGCCACAACCCGTCGGACTACAACGGTTTCAAGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATCGAAGCCCTGGGCTGCGAAGTGATCCCGCT  >  NC_002947/6035789‑6036075
                                                                                                                                                |                                                                                                                                              
ggCAGCCACAACCCGTCGGACTACAACGGTTTCAAGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCt                                                                                                                                            >  2:118932/1‑149 (MQ=255)
     ccACAACCCGTCGGACTACAACGGTTTCAAGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGGATCTCGAcc                                                                                                                                       <  8:140235/149‑1 (MQ=255)
          aCCCGTCGGACTACAACGGTTTCAACATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGGATCTCGAccttttc                                                                                                                                  <  2:87355/149‑6 (MQ=255)
            ccGTCGGACTACAACGGTTTCAAGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGCCCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGCTCCTCGCCCGCTACtt                                                                                                                                >  2:56476/1‑149 (MQ=255)
                 ggACTACAACGGTTTCAAGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGGCGAGATCCTCGACCGCTACTTCAAg                                                                                                                            <  1:50169/148‑1 (MQ=255)
                     tACAACGGTTTCAAGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAg                                                                                                                            >  7:152254/1‑144 (MQ=255)
                             tttCAAGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGc                                                                                                                >  6:106405/1‑148 (MQ=255)
                                cAAGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACg                                                                                                            >  6:199810/1‑149 (MQ=255)
                                 aaGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGc                                                                                                                           >  1:161928/1‑133 (MQ=255)
                                 aaGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGc                                                                                                                           <  2:161928/133‑1 (MQ=255)
                                 aaGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACg                                                                                                            <  7:187104/148‑1 (MQ=255)
                                        tCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTg                                                                                                    <  1:118932/149‑1 (MQ=255)
                                         cATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCt                                                                                                                                    <  6:25463/116‑1 (MQ=255)
                                         cATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCt                                                                                                                                    >  5:25463/1‑116 (MQ=255)
                                                ggTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGaaaaa                                                                                            <  5:199810/149‑1 (MQ=255)
                                                                  gAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGc                                                                                                  <  4:198225/125‑1 (MQ=255)
                                                                  gAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGc                                                                                                  >  3:198225/1‑125 (MQ=255)
                                                                  gAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGTTGGa                                                                          >  4:34779/1‑149 (MQ=255)
                                                                  gAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGa                                                                          >  5:98882/1‑149 (MQ=255)
                                                                  gAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGa                                                                          >  4:103846/1‑149 (MQ=255)
                                                                    aCAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACGCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACt                                                                        >  1:229357/1‑149 (MQ=255)
                                                                     cAGATCCAGGCCCTGCTGCCCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTg                                                                       >  3:299233/1‑149 (MQ=255)
                                                                             ggCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAAcc                                                               <  3:299309/149‑2 (MQ=255)
                                                                              gCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGTTCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTTGACTGCGGCAActg                                                              <  3:34779/149‑3 (MQ=255)
                                                                                      gACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGgc                                                             >  3:276725/1‑142 (MQ=255)
                                                                                      gACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGgc                                                             <  4:276725/142‑1 (MQ=255)
                                                                                            ccTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTgg                                                <  2:229357/149‑1 (MQ=255)
                                                                                                aaaaCCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACgg                                                              >  5:156354/1‑131 (MQ=255)
                                                                                                aaaaCCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACgg                                                              <  6:156354/131‑1 (MQ=255)
                                                                                                aaaaCCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTg                                                 <  8:102240/144‑1 (MQ=255)
                                                                                                aaaaCCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTg                                                 >  7:102240/1‑144 (MQ=255)
                                                                                                 aaaCCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCa                                           <  3:103846/149‑1 (MQ=255)
                                                                                                  aaCCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCAc                                          >  7:18666/1‑149 (MQ=255)
                                                                                                     cAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCg                                        >  3:28372/1‑148 (MQ=255)
                                                                                                     cAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCg                                        >  4:288705/1‑148 (MQ=255)
                                                                                                       aCGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCaa                                     <  5:106405/149‑1 (MQ=255)
                                                                                                             tGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAATTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATc                               >  8:232393/1‑149 (MQ=255)
                                                                                                              gACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATc                               >  5:53460/1‑148 (MQ=255)
                                                                                                              gACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATc                               >  3:177407/1‑148 (MQ=255)
                                                                                                               aCCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATc                               <  3:288705/147‑1 (MQ=255)
                                                                                                                         aaGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATCGAAGCCCTGGGc                   <  4:28372/149‑1 (MQ=255)
                                                                                                                               gcgTGGAAAAGGTCGAGATCCTCGAACGCTACTTCAAGCAGATGGTCGGCGAGGTGAAGCTGGGGAAAAAGCTCAAAGTGGTGGTGGACTGCTGCAACGGCGCCGCTGTCGTGGTTGCACCGCAAGTGATCGAAGCCCTGGGCTGCGaa             <  2:25553/149‑1 (MQ=255)
                                                                                                                                      agcggTCGAGGATCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATCGAAGCCCTGGGCTGCGAAGTGATcc      >  5:45349/4‑149 (MQ=255)
                                                                                                                                        aGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATCGAAGCCCTGGGCTGCGAAGTGATCCCg    >  1:95932/1‑149 (MQ=255)
                                                                                                                                         ggTCGAGGATCTCGACCGCTACTTCTAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGCTCGAAGCCCTGGGCTGCGAAGTGATCCCGc   >  8:221629/1‑149 (MQ=255)
                                                                                                                                         ggTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATCGAAGCCCTGGGCTGCGAAGTGATCCCGc   <  1:56476/149‑1 (MQ=255)
                                                                                                                                          gTCGAGATCCTCGCCCGCTACTTCAAGCAGATCGTCGGCCCCGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGATGCACCGCAACTGATCGAAGCCCTGGGCTGCGAAGTGATCCCGCt  >  1:57016/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GGCAGCCACAACCCGTCGGACTACAACGGTTTCAAGATCGTCATCGCCGGTGACACCCTGGCCAACGAACAGATCCAGGCCCTGCTGACCCGCCTGAAAACCAACGACCTGACCCTGGCCCAAGGCCGCGTGGAAAAGGTCGAGATCCTCGACCGCTACTTCAAGCAGATCGTCGGCGACGTGAAGCTGGCGAAAAAGCTCAAAGTGGTGGTGGACTGCGGCAACGGCGCCGCTGGCGTGGTTGCACCGCAACTGATCGAAGCCCTGGGCTGCGAAGTGATCCCGCT  >  NC_002947/6035789‑6036075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑