Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,041,143 T→A 8.8% N12I (AAT→ATT)  PP_5293 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,041,1430TA 8.8% 185.2 / 8.0 68N12I (AAT→ATT) PP_5293hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/30);  new base A (3/3);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

CTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGCTGG  >  NC_002947/6040998‑6041291
                                                                                                                                                 |                                                                                                                                                    
ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAATGCCGAAATCCGGCAATgg                                                                                                                                                   <  2:259426/149‑1 (MQ=255)
  ctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGc                                                                                                                                                 >  3:67688/1‑149 (MQ=255)
   tttGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCg                                                                                                                                                >  8:166489/1‑149 (MQ=255)
            ggCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATc                                                                                                                                        <  7:166489/148‑1 (MQ=255)
               gTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAACGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACa                                                                                                                                    <  2:74590/149‑1 (MQ=255)
                tCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAg                                                                                                                                   >  5:279906/1‑149 (MQ=255)
                   aTAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGtt                                                                                                                                <  5:172492/149‑1 (MQ=255)
                    tAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAATGCCGAAATCCGGCAATggg                                                                                                                                                  >  8:214122/1‑130 (MQ=255)
                    tAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAATGCCGAAATCCGGCAATggg                                                                                                                                                  <  7:214122/130‑1 (MQ=255)
                      aaaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGa                                                                                                                             <  6:198913/149‑1 (MQ=255)
                      aaaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGa                                                                                                                             <  2:146446/149‑1 (MQ=255)
                       aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                            >  5:239670/1‑149 (MQ=255)
                        aGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAAt                                                                                                                           <  1:2093/149‑1 (MQ=255)
                          ggTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCg                                                                                                                         <  1:183802/149‑1 (MQ=255)
                                gCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCa                                                                                                                     <  6:144620/147‑1 (MQ=255)
                                      gcTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGc                                                                                                             <  5:291097/149‑1 (MQ=255)
                                           aGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcctc                                                                                                        <  5:217653/149‑1 (MQ=255)
                                             gTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcctcct                                                                                                      >  8:92503/1‑149 (MQ=255)
                                              tGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGCGGTGATCGACAGGTTCGACTCGCTCATGCCCCGCACCTCCtt                                                                                                     >  5:171062/1‑149 (MQ=255)
                                                 cATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGc                                                                                                  >  5:204576/1‑149 (MQ=255)
                                                 cATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGc                                                                                                  >  4:288226/1‑149 (MQ=255)
                                                  aTAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGcc                                                                                                 >  1:302946/1‑149 (MQ=255)
                                                       aCCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGttt                                                                                            >  3:125826/1‑149 (MQ=255)
                                                        ccAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCCCCTCCTTGGCCGGttt                                                                                            >  1:9774/1‑148 (MQ=255)
                                                         cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGttt                                                                                            >  5:330824/1‑147 (MQ=255)
                                                         cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGttt                                                                                            <  6:330824/147‑1 (MQ=255)
                                                           gCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAATGCCGAAATCCGGCAATGGGCgat                                                                                                                                              <  7:142620/95‑3 (MQ=37)
                                                           gCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAATGCCGAAATCCGGCAATGGGCgat                                                                                                                                              >  8:142620/1‑93 (MQ=37)
                                                               gCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGc                                                                                    <  6:239670/149‑1 (MQ=255)
                                                                 ccAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGc                                                                                    >  7:286243/1‑147 (MQ=255)
                                                                   agcagGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGt                                                                                >  4:176128/1‑149 (MQ=255)
                                                                   agcagGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGt                                                                                >  5:78280/1‑149 (MQ=255)
                                                                         tGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcc                                                                          <  4:24674/149‑1 (MQ=255)
                                                                             cAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgct                                                                      >  7:4957/1‑149 (MQ=255)
                                                                             cAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgct                                                                      >  8:154278/1‑149 (MQ=255)
                                                                              aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGttt                                                                                            >  5:186051/1‑126 (MQ=255)
                                                                              aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGttt                                                                                            <  6:186051/126‑1 (MQ=255)
                                                                               aGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcctg                                                                        <  3:274291/145‑1 (MQ=255)
                                                                               aGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcctg                                                                        >  4:274291/1‑145 (MQ=255)
                                                                                       tACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGa                                                             >  1:22941/1‑148 (MQ=255)
                                                                                         ccAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaaaa                                                          <  2:302946/149‑1 (MQ=255)
                                                                                          cAGCCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAt                                                         <  2:111684/149‑1 (MQ=255)
                                                                                                   cAGCGCGGAGAGGTGGCAGAACTTCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCAt                                                <  2:41616/149‑1 (MQ=255)
                                                                                                    aGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAt                                                         <  6:310350/139‑1 (MQ=255)
                                                                                                    aGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAt                                                         >  5:310350/1‑139 (MQ=255)
                                                                                                      cgcgGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGcc                                             <  8:4957/149‑1 (MQ=255)
                                                                                                       gcgGAGAGGTGGCAGAACATAGCCCATTGCAGGATTTCGGAATTGGGCGGGGTGAACGACAGGTTAGAATCGCTCATGCACCGCACCTCCTTCGACGGTTTCACTCAGCAAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTACATGcc                                             >  2:164879/1‑148 (MQ=255)
                                                                                                         ggAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCtt                                          <  2:22941/149‑1 (MQ=255)
                                                                                                              ggTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTgc                                      <  6:204576/148‑1 (MQ=255)
                                                                                                                  gCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCa                                  >  6:190943/1‑148 (MQ=255)
                                                                                                                    aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAg                               >  5:291150/1‑149 (MQ=255)
                                                                                                                    aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGGTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTACTGCGCCAAAg                               >  1:235453/1‑149 (MQ=255)
                                                                                                                     gAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGc                              <  6:279906/149‑1 (MQ=255)
                                                                                                                       aCATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGa                            <  2:9774/149‑1 (MQ=255)
                                                                                                                         aTCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGc                          <  5:190943/149‑1 (MQ=255)
                                                                                                                           cGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCAt                        >  3:229771/1‑149 (MQ=255)
                                                                                                                           cGCCCAATGCCGAAATCCGGCAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCAt                        >  1:319403/1‑149 (MQ=255)
                                                                                                                              ccATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGc                     <  3:288226/149‑1 (MQ=255)
                                                                                                                               cATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGGCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCg                    >  1:224290/1‑149 (MQ=255)
                                                                                                                                aTTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGt                   >  8:243939/1‑149 (MQ=255)
                                                                                                                                aTTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGt                   <  4:125826/149‑1 (MQ=255)
                                                                                                                                    ccGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGa                >  3:227371/1‑148 (MQ=255)
                                                                                                                                      ggATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCg                                                     >  6:145702/1‑109 (MQ=255)
                                                                                                                                      ggATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCg                                                     <  5:145702/109‑1 (MQ=255)
                                                                                                                                            cGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTcc        <  4:67688/148‑1 (MQ=255)
                                                                                                                                            cGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCATCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCg       >  8:117/1‑149 (MQ=255)
                                                                                                                                              gCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGc     <  7:154278/149‑1 (MQ=255)
                                                                                                                                              gCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGc     >  1:35810/1‑149 (MQ=255)
                                                                                                                                                 ttGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGCTgg  <  4:227371/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGCTGG  >  NC_002947/6040998‑6041291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑