Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,057,540 G→A 5.5% S246N (AGC→AAC)  recG → junction‑specific ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,057,5400GA 5.5% 223.9 / 2.1 73S246N (AGC→AAC) recGjunction‑specific ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (35/34);  new base A (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GAAGCCATCCGCTACCTGCACAACCCGCCGGCCGACGCTGACCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCGATGATGCGTCTG  >  NC_002947/6057392‑6057685
                                                                                                                                                    |                                                                                                                                                 
gAAGCCATCCGCTACCTGCACAACCCGCCGGCCGACGCTGACCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAg                                                                                                                                                   >  2:173660/1‑149 (MQ=255)
     cATCCGCTACCTGCACAACCCGCCGGCCGACGCTGACCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCtgc                                                                                                                                              >  5:222242/1‑149 (MQ=255)
     cATCCCCTACCTGCACAACCCGCCGGCCGACGCTGACCTCTTCTAGCTCGCCGCAGGCCAGCACTGGTCTCAACTCCGCCTGGCCTTCGAAGAGTTGCTGTCCCTTCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCtgc                                                                                                                                              <  2:63808/149‑1 (MQ=255)
           cTACCTGCACAACCCGCCGGCCGACGCTGACCTCGACGAGCACGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTgcgc                                                                                                                                        <  5:277010/149‑1 (MQ=255)
               cTGCACAACCCGCCGGCCGACGCTGACCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCgg                                                                                                                                     >  3:180763/1‑148 (MQ=255)
                  cacaACCCGCCGGCCGACGCTGACCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTa                                                                                                                                   <  3:220449/147‑1 (MQ=255)
                       cccGCCGGCCGACGCTGAACTCGACGAGGTCGCCGAAGGCCAGCAATTGGCTCAACACCTCCTGTTCTTCTAAGAGTTTCTTACCCTTCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCa                                                                                                                            <  2:308692/149‑1 (MQ=255)
                        ccgccgGCCGACGCTGACCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTAc                                                                                                                                  <  1:258211/142‑1 (MQ=255)
                        ccgccgGCCGACGCTGACCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTAc                                                                                                                                  >  2:258211/1‑142 (MQ=255)
                                    gCTGACCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCt                                                                                                               <  6:286842/149‑1 (MQ=255)
                                        aCCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAg                                                                                                           <  7:243532/149‑1 (MQ=255)
                                          cTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCtgcgt                                                                                                                                            <  4:318718/114‑1 (MQ=255)
                                          cTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCtgcgt                                                                                                                                            >  3:318718/1‑114 (MQ=255)
                                          cTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGgc                                                                                                         >  2:2698/1‑149 (MQ=255)
                                             gacgaGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGgcgc                                                                                                       <  2:323354/148‑1 (MQ=255)
                                              acgaGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCaa                                                                                                     >  6:152512/1‑149 (MQ=255)
                                                gaGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGgcgcgtgg                                                                                                   <  1:173660/149‑5 (MQ=255)
                                                gaGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATa                                                                                                   >  1:1808/1‑149 (MQ=255)
                                                  gCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGAGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATAcc                                                                                                 >  1:255758/1‑149 (MQ=255)
                                                       ccGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGc                                                                                             >  7:140898/1‑148 (MQ=255)
                                                              ccAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTgg                                                                                     <  1:119552/149‑1 (MQ=255)
                                                               cAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTggg                                                                                    >  7:274980/1‑149 (MQ=255)
                                                                aGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGc                                                                                   >  3:53895/1‑149 (MQ=255)
                                                                      gggCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAg                                                                             >  5:246973/1‑149 (MQ=255)
                                                                       ggCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAg                                                                             <  1:286110/148‑1 (MQ=255)
                                                                               acCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGgc                                                                    >  5:210741/1‑149 (MQ=255)
                                                                               acCGCCTGGCCTTCGAAGAGTTGCTGACCCATCACCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGgc                                                                    <  4:180763/149‑1 (MQ=255)
                                                                                    cTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGgcgcg                                                                 >  4:95990/1‑147 (MQ=255)
                                                                                       gCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGCGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCgcag                                                              <  2:255758/147‑1 (MQ=255)
                                                                                         cTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCagcagc                                                          >  1:196482/1‑149 (MQ=255)
                                                                                         cTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGAGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCagcagc                                                          <  8:189831/149‑1 (MQ=255)
                                                                                         cTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCACCGGTACTGCCCAGGGACACGCGCCTGCAGGCGCAATACCAGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCagcagc                                                          >  5:156572/1‑149 (MQ=255)
                                                                                          ttCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCg                                                         >  8:1015/1‑149 (MQ=255)
                                                                                            cGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCgg                                                        <  6:210741/148‑1 (MQ=255)
                                                                                            cGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCg                                                         <  8:274980/147‑1 (MQ=255)
                                                                                               agagTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAg                                                    <  2:322519/149‑1 (MQ=255)
                                                                                                gagTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGc                                                   <  1:2698/149‑1 (MQ=255)
                                                                                                   ttGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCaa                                                <  6:246973/149‑1 (MQ=255)
                                                                                                   ttGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCaa                                                >  6:238516/1‑149 (MQ=255)
                                                                                                         aCCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGaa                                            <  7:1015/147‑1 (MQ=255)
                                                                                                          cccATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAAtt                                         <  5:152512/149‑1 (MQ=255)
                                                                                                           ccATCAGCTGTCGCAGCAACGCCTGCGCGAAGGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTg                                        >  1:69734/1‑149 (MQ=255)
                                                                                                           ccATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTg                                        >  7:185787/1‑149 (MQ=255)
                                                                                                           ccATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTg                                        >  7:178444/1‑149 (MQ=255)
                                                                                                            cATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGc                                       >  5:293975/1‑149 (MQ=255)
                                                                                                             aTCAGCTCTCGCAGCAACGCCTCCGCCAAAGCCTGCGCAGCCTGCGTCCGCCGCAACTGCCCAGCCCCACGCCCCAGCAGGCGCAATACCAGGCAAACCTGCGCTACCAGCCGACCGGCGCGCACCAGCGGGTAGCCAACGAAAtagac                                      >  5:59951/1‑145 (MQ=255)
                                                                                                              tCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGt                                                      >  5:197922/1‑132 (MQ=255)
                                                                                                              tCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGt                                                      <  6:197922/132‑1 (MQ=255)
                                                                                                              tCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCt                                     <  3:95990/149‑1 (MQ=255)
                                                                                                                aGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTAc                                   <  4:53895/149‑1 (MQ=255)
                                                                                                                 gatGTCGCAGCAACTCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCAAGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGAAGCAGCGGGTAGCCAACGAAATTGCGTAc                                   >  6:105571/3‑148 (MQ=255)
                                                                                                                 gCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCACAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACg                                  >  3:3707/1‑149 (MQ=255)
                                                                                                                  cTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGa                                 >  4:293516/1‑149 (MQ=255)
                                                                                                                    gTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGTAGCGGGTAGCCAACGa                                             <  6:12342/135‑1 (MQ=255)
                                                                                                                    gTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGTAGCGGGTAGCCAACGa                                             >  5:12342/1‑135 (MQ=255)
                                                                                                                     tCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGAc                                <  8:185787/147‑1 (MQ=255)
                                                                                                                       gcagcaACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCACAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCtt                             <  4:3707/148‑1 (MQ=255)
                                                                                                                        cagcaACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAg                           >  5:221414/1‑149 (MQ=255)
                                                                                                                            aaCGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAg                       <  6:221414/149‑1 (MQ=255)
                                                                                                                            aaCGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAg                       <  8:178444/149‑1 (MQ=255)
                                                                                                                              cGCCTGCGCGAAAGCCTGCGCCGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTCGGCTTCCCGCCGCCCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCa                     >  5:302714/1‑149 (MQ=255)
                                                                                                                                    cgcgAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAAc                <  8:140898/148‑1 (MQ=255)
                                                                                                                                      cgAAAGCCTGCGGAGCCTGCGTGCGCGGGTACTGGCCTGGGCCACGCGCCTGCAGGGGCAATACCTGGCTAAGCTGGGCTTCCAGCGGACGGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCGTACGACCTTAGCCTGCACGAACGga             <  6:59951/149‑1 (MQ=255)
                                                                                                                                      cgAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCga             <  4:146542/149‑1 (MQ=255)
                                                                                                                                       gAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCgat            >  7:166924/1‑149 (MQ=255)
                                                                                                                                       gAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCCTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCgat            >  4:116509/1‑149 (MQ=255)
                                                                                                                                           gCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGGAGCAGCGGGTAGCAAACGAAATTGCCTACGACCTTAGCCAGCACGAACCGatgatg        >  1:242324/1‑149 (MQ=255)
                                                                                                                                            ccTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCGATGATGc       >  7:133728/1‑149 (MQ=255)
                                                                                                                                            ccTGCCCAGCCTTCGTTCGCCTTTACTTCCCATGTCAACGCTCCTGAAGGCGCAATACCTGGCTAACCTGGGCTTCCATCCGACCGGCGCGCAGCAGCGGGTAGCCAACTAAATTGCCTACGACCTTAGCCAGCACGAACCGATGATGc       <  5:105571/149‑1 (MQ=255)
                                                                                                                                             cTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCACGCGCAATACCAGGCTAACCTCGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTCGCCAGCCCGAACCGAGGATGCg      >  8:106290/1‑149 (MQ=255)
                                                                                                                                              tGCGCAACCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCGATGATGCg      >  4:298027/1‑148 (MQ=255)
                                                                                                                                              tGCGCAACCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCGATGATGCGt     >  4:302731/1‑149 (MQ=255)
                                                                                                                                                 gcAACCTGCGTGCTCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCGATGATGCGTCTg  <  3:298027/149‑1 (MQ=255)
                                                                                                                                                 gcAACCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCGATGATGCGTCTg  <  3:302731/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GAAGCCATCCGCTACCTGCACAACCCGCCGGCCGACGCTGACCTCGACGAGCTCGCCGAAGGCCAGCACTGGGCTCAACACCGCCTGGCCTTCGAAGAGTTGCTGACCCATCAGCTGTCGCAGCAACGCCTGCGCGAAAGCCTGCGCAGCCTGCGTGCGCCGGTACTGCCCAGGGCCACGCGCCTGCAGGCGCAATACCTGGCTAACCTGGGCTTCCAGCCGACCGGCGCGCAGCAGCGGGTAGCCAACGAAATTGCCTACGACCTTAGCCAGCACGAACCGATGATGCGTCTG  >  NC_002947/6057392‑6057685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑