Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,086,776	A→T	8.0%	M100L (ATG→TTG)	oruR →	ornithine utilization transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,086,776	0	A	T	8.0%	144.9 / 2.4	50	M100L (ATG→TTG)	oruR	ornithine utilization transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base T (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

CGAGGCGTCGGCGTTGCTGGCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTCGACGACGGCCAACGTGTCTGGT  >  NC_002947/6086628‑6086923                                                                                                                                                        |                                                                                                                                                    cGAGGCGTCGGCGTTGCTGGCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGTAACTGGGCCAGTGCa                                                                                                                                                     <  8:228638/149‑1 (MQ=255)    ggCGTCGGCGTTGCTGGCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATgc                                                                                                                                                  <  7:255264/149‑1 (MQ=255)      cGTCGGCGTTGCTGGCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATgcgc                                                                                                                                                <  6:119503/149‑1 (MQ=255)          ggCGTTGCTGGCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCgtgt                                                                                                                                            <  3:307034/149‑1 (MQ=255)                   ggCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATgcgc                                                                                                                                                >  3:317124/1‑136 (MQ=255)                   ggCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATgcgc                                                                                                                                                <  4:317124/136‑1 (MQ=255)                    gCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACgg                                                                                                                                  >  4:17649/1‑149 (MQ=255)                    gCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACgg                                                                                                                                  <  7:41702/149‑1 (MQ=255)                            cGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCAt                                                                                                                                                    <  6:328656/123‑1 (MQ=255)                            cGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCAt                                                                                                                                                    >  5:328656/1‑123 (MQ=255)                                      ccGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATg                                                                                                                                                   <  6:170689/114‑1 (MQ=255)                                       cGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGGAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGGCTGGAGCTGGGACAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCgctg                                                                                                               >  4:231442/1‑149 (MQ=255)                                         gCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCgctgct                                                                                                             >  2:100961/1‑149 (MQ=255)                                                tGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGt                                                                                                      >  8:70773/1‑149 (MQ=255)                                                   ggCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGcc                                                                                                   <  3:17649/149‑1 (MQ=255)                                                    gCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCa                                                                                                  >  2:30093/1‑149 (MQ=255)                                                    gCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCa                                                                                                  <  1:30093/149‑1 (MQ=255)                                                         ccGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGCGCCCGCCCACCCCCCCCAGATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCAc                                                                                                 <  1:269044/148‑1 (MQ=255)                                                          cGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCTTGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCtctc                                                                                                          <  1:15403/135‑1 (MQ=255)                                                          cGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCTTGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCtctc                                                                                                          <  1:10126/135‑1 (MQ=255)                                                          cGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCTTGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCtctc                                                                                                          >  2:10126/1‑135 (MQ=255)                                                          cGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCTTGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCtctc                                                                                                          >  2:15403/1‑135 (MQ=255)                                                          cGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTgg                                                                                            >  1:198358/1‑149 (MQ=255)                                                          cGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTgg                                                                                            >  5:210522/1‑149 (MQ=255)                                                                         caccCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCTCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGcc                                                                             >  4:54960/1‑149 (MQ=255)                                                                           cccAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTg                                                                           >  1:35739/1‑149 (MQ=255)                                                                                 agGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACTTTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTg                                                                     <  2:35739/149‑1 (MQ=255)                                                                                       tGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTAt                                                               >  4:1845/1‑149 (MQ=255)                                                                                       tGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCATGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTAt                                                               >  6:13856/1‑149 (MQ=255)                                                                                        gCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATc                                                              >  6:159056/1‑149 (MQ=255)                                                                                            gTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCgg                                                           >  3:14878/1‑148 (MQ=255)                                                                                            gTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCgg                                                           >  7:304647/1‑148 (MQ=255)                                                                                             tttGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTTCAGTATCCGGCa                                                         <  3:54960/149‑1 (MQ=255)                                                                                             tttGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCa                                                         >  5:8395/1‑149 (MQ=255)                                                                                                 cgcCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCActgc                                                     <  5:13856/149‑1 (MQ=255)                                                                                                   ccAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCActgctg                                                   <  4:148201/149‑1 (MQ=255)                                                                                                    cAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTgg                                                  <  5:159056/149‑1 (MQ=255)                                                                                                       tGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGaa                                               >  5:310411/1‑149 (MQ=255)                                                                                                       tGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGaa                                               <  6:210522/149‑1 (MQ=255)                                                                                                                  cccGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTc                                       <  8:87208/149‑1 (MQ=255)                                                                                                                   ccGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCa                                      <  2:198358/149‑1 (MQ=255)                                                                                                                           aGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTgcgc                              <  3:1845/149‑1 (MQ=255)                                                                                                                           aGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTgcgc                              <  4:14878/149‑1 (MQ=255)                                                                                                                                   ggCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTcg                       <  6:310411/148‑1 (MQ=255)                                                                                                                                       tGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTCgacgac                  >  2:95804/1‑149 (MQ=255)                                                                                                                                       tGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTCgacgac                  >  5:270531/1‑149 (MQ=255)                                                                                                                                        ggAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTCGacgacg                 <  7:70773/149‑1 (MQ=255)                                                                                                                                         gAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTCGacgacg                 <  5:287137/148‑1 (MQ=255)                                                                                                                                                 ccAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTCGACGACGGCCAACgtg        >  8:174289/1‑149 (MQ=255)                                                                                                                                                      gcATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTCGACGACGGCCAACGTGTCTgg   <  3:231442/149‑1 (MQ=255)                                                                                                                                                       cATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTCGACGACGGCCAACGTGTCTGGt  <  6:8395/149‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                    CGAGGCGTCGGCGTTGCTGGCCGGCAGCGGCATTGGCCCGGCAGACCTGGGGCATGCCGATGCGCGCATCACCACCCAGCAGGAGCTGCAGGTTTGCGCCAATGCTGTG‑‑‑GCCCGGCGCGAGGACATTGGCCTGGAACTGGGCCAGCGCATGCGCGTGTCGTGCTACGGCATGCTCGGTTACGCGCTGCTCTCCAGTGCCACTTTGGGTGACGCCCTACGCCTGGCGCTGCAGTATCCGGCACTGCTGGGAACAGTCTTCAAGCTGCGCTTGCTCGACGACGGCCAACGTGTCTGGT  >  NC_002947/6086628‑6086923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑