Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,114,219 A→T 6.9% E450V (GAA→GTA)  clsA → cardiolipin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,114,2190AT 6.9% 163.3 / 3.1 58E450V (GAA→GTA) clsAcardiolipin synthase
Reads supporting (aligned to +/- strand):  ref base A (29/24);  new base T (2/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

TGGTGCTGGTGGATGATGACGTCAGCGCAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTTTTCGCGCTAAAGGCCGCTC  >  NC_002947/6114075‑6114363
                                                                                                                                                |                                                                                                                                                
tggtgCTGGTGGATGATGACGTCAGCGCAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACaa                                                                                                                                              <  5:106063/149‑1 (MQ=255)
 ggtgCTGGTGGATGATGACGTCAGCGCAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAg                                                                                                                                             >  3:262409/1‑149 (MQ=255)
   tgCTGGTGGATGATGACGTCAGCGCAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAgcg                                                                                                                                           >  8:252534/1‑149 (MQ=255)
      tggtggATGATGACGTCAGCGCAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCgg                                                                                                                                        >  5:156783/1‑149 (MQ=255)
               atgaCGTCAGCGCAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTAct                                                                                                                               <  1:63484/149‑1 (MQ=255)
                tgaCGTCAGCGCAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTAct                                                                                                                               <  6:156783/148‑1 (MQ=255)
                    gTCAGCGCAATTGGTAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCTAACAAGCGCGGGAAATTACTGCTGa                                                                                                                          <  7:252534/149‑1 (MQ=255)
                    gTCAGCGCAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTActgctg                                                                                                                           >  2:7709/1‑148 (MQ=255)
                        gcgcAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTActgc                                                                                                                             >  5:218754/1‑142 (MQ=255)
                        gcgcAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTActgc                                                                                                                             <  6:218754/142‑1 (MQ=255)
                                   aGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATAc                                                                                                           <  8:14401/149‑1 (MQ=255)
                                     tGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATAcc                                                                                                          >  6:244542/1‑148 (MQ=255)
                                       ccAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAAt                                                                                                                                   >  6:81587/1‑121 (MQ=255)
                                       ccAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAAt                                                                                                                                   <  5:81587/121‑1 (MQ=255)
                                         aaCCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATc                                                                                                      >  7:31973/1‑148 (MQ=255)
                                               gACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTgca                                                                                               <  4:262409/149‑1 (MQ=255)
                                                    ccGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAAt                                                                                          >  2:142362/1‑149 (MQ=255)
                                                                ccTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGa                                                                              <  5:244542/149‑1 (MQ=255)
                                                                 cTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGAt                                                                             >  5:64578/1‑149 (MQ=255)
                                                                   gAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTg                                                                           >  7:128733/1‑149 (MQ=255)
                                                                    aaCTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAATACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCTGAtt                                                                            <  6:106141/147‑1 (MQ=255)
                                                                        tCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGTACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCg                                                                                >  4:143358/1‑139 (MQ=255)
                                                                        tCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGTACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCg                                                                                >  4:148233/1‑139 (MQ=255)
                                                                        tCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGTACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCg                                                                                <  3:148233/139‑1 (MQ=255)
                                                                        tCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGTACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCg                                                                                <  3:143358/139‑1 (MQ=255)
                                                                           agaTTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGAtt                                                                            <  2:20155/140‑1 (MQ=255)
                                                                           agaTTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGAtt                                                                            >  1:20155/1‑140 (MQ=255)
                                                                                  ccTGCTGACAGTCGATCGCGGCTTCGCCGCCCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCACCAATAGGGGATGCGGATTGCGCGGCTGATATCGc                                                            >  6:18133/1‑149 (MQ=255)
                                                                                  ccTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGc                                                            >  4:146487/1‑149 (MQ=255)
                                                                                  ccTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGc                                                            >  4:264364/1‑149 (MQ=255)
                                                                                        gACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTc                                                      >  1:74331/1‑149 (MQ=255)
                                                                                         aCAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTc                                                      <  1:7709/148‑1 (MQ=255)
                                                                                           aGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCttt                                                   <  8:31973/149‑1 (MQ=255)
                                                                                            gTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTa                                                  <  3:146487/149‑1 (MQ=255)
                                                                                              cGATCGCGGTTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAgg                                                <  5:146429/149‑1 (MQ=255)
                                                                                               gATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAgg                                                <  2:74331/148‑1 (MQ=255)
                                                                                                 tCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTg                                             <  1:275039/149‑1 (MQ=255)
                                                                                                            ccGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGt                                  >  5:315927/1‑149 (MQ=255)
                                                                                                               aCCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTg                                >  6:57535/1‑148 (MQ=255)
                                                                                                               aCCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTg                                >  4:105499/1‑148 (MQ=255)
                                                                                                                ccAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGaa                              <  8:128733/149‑1 (MQ=255)
                                                                                                                  aGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAg                            >  4:208776/1‑149 (MQ=255)
                                                                                                                   ggTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGc                           <  3:105499/149‑1 (MQ=255)
                                                                                                                    gTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCg                          <  6:64578/149‑1 (MQ=255)
                                                                                                                    gTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAACAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCg                          >  1:198076/1‑149 (MQ=255)
                                                                                                                      cGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGc                        <  3:208776/149‑1 (MQ=255)
                                                                                                                            caTGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTTTTc                  <  3:264364/149‑1 (MQ=255)
                                                                                                                             aTGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTTTTcg                 <  1:142362/149‑1 (MQ=255)
                                                                                                                                    aGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACATGCCCGTTGAAAGCGGCCTTTTCGCGCTa            >  4:189463/1‑147 (MQ=255)
                                                                                                                                    aGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTTTTCGCGCTa            >  7:197276/1‑147 (MQ=255)
                                                                                                                                     ggAAGACTACGCACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTCCAGCAATTGGGGATGCGGATTCCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTCAACGCCGCCATTACGCGCTAAAg         >  4:267065/1‑149 (MQ=255)
                                                                                                                                      gAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGCCTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTATTCGCGCTAAAgg        >  7:72983/1‑149 (MQ=255)
                                                                                                                                      gAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTTTTCGCGCTAAAgg        >  7:122727/1‑149 (MQ=255)
                                                                                                                                      gAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTTTTCGCGCTAAAgg        >  1:5291/1‑149 (MQ=255)
                                                                                                                                            ttCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGcc                       <  5:209196/128‑1 (MQ=255)
                                                                                                                                            ttCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGcc                       >  6:209196/1‑128 (MQ=255)
                                                                                                                                            ttCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTTTTCGCGCTAAAGGCCGCTc  >  2:215797/1‑149 (MQ=255)
                                                                                                                                            ttCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTTTTCGCGCTAAAGGCCGCTc  >  6:138800/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGGTGCTGGTGGATGATGACGTCAGCGCAATTGGGAGTGCCAACCTGGACAACCGCTCGTTCCGCCTGAACTTCGAGATTACCCTGCTGACAGTCGATCGCGGCTTCGCCGACCAGGTCGAGCACATGCTCCAGGAAGACTTCGAACAAGCGCGGGAAATTACTGCTGAAGACACCCAAGATACCCATCGACTGCAGCAATTGGGGATGCGGATTGCGCGGCTGATATCGCCGATTCTTTAGGGTGAACACGCCCGTTGAAAGCGGCCTTTTCGCGCTAAAGGCCGCTC  >  NC_002947/6114075‑6114363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑