| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 496,096 | T→A | 12.5% | intergenic (‑63/‑33) | PP_0408 ← / → PP_0409 | hypothetical protein/two‑component system sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 496,096 | 0 | T | A | 12.5% | 170.7 / 11.6 | 69 | intergenic (‑63/‑33) | PP_0408/PP_0409 | hypothetical protein/two‑component system sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base T (27/33); new base A (3/6); total (30/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGCTGGAGCCTGACCAGCAGCAGTGG > NC_002947/495955‑496242 | actCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGc > 1:14162/3‑149 (MQ=255)actCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGc > 5:222843/3‑149 (MQ=255) aTCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGcc < 1:16203/149‑1 (MQ=255) tCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCg < 5:161449/149‑1 (MQ=255) cggcggCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGt > 5:223097/1‑149 (MQ=255) gcggcCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGTca < 3:166423/149‑2 (MQ=255) cggcCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACCTTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCtt < 2:105661/149‑1 (MQ=255) cggcCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCtt < 4:152360/149‑1 (MQ=255) cggcCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGTcac < 6:223097/149‑3 (MQ=255) cggcCAGGGCGCCGAGTGGAACGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGTcac < 2:181793/149‑3 (MQ=255) ggcCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCttt < 3:200177/149‑1 (MQ=255) aGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGcc > 2:266694/1‑148 (MQ=255) gcgcCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGt < 3:57125/149‑1 (MQ=255) ccGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGa < 6:247740/149‑1 (MQ=255) gAGTGTTAGGACCGAGGCAAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGTACATGAGTCAACCAAAGGCCAGAATGAAAGCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGc < 6:173520/149‑1 (MQ=255) gAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGtata < 4:246280/149‑1 (MQ=255) aGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCGAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGa < 3:260737/149‑1 (MQ=255) aCCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTCCATGGCCGTCTATGCCAAGTCGAGCGTATAGATg > 5:258083/1‑148 (MQ=255) ccGAGGCCAGGCAGCACATTGCTAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGACATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATg > 7:201565/1‑147 (MQ=255) ccGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGt < 5:90600/122‑1 (MQ=255) ccGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGt < 5:95153/122‑1 (MQ=255) ccGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGt > 6:95153/1‑122 (MQ=255) ccGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGt > 6:90600/1‑122 (MQ=255) gAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGc > 6:184541/1‑149 (MQ=255) ccAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCGAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGt > 7:62782/1‑149 (MQ=255) cacaTTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGcc > 6:50211/1‑149 (MQ=255) acaTTGCCAACGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCt > 1:238007/1‑149 (MQ=255) aTTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGt > 5:172984/1‑149 (MQ=255) ttGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCATAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGtt < 5:235761/149‑1 (MQ=255) ttGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGtt > 5:49363/1‑149 (MQ=255) gCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTg < 6:49363/148‑1 (MQ=255) ccAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGt > 5:75497/1‑149 (MQ=255) cAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGtt < 1:287222/149‑1 (MQ=255) aGCTTCGTGCGATAAAGTGTGGACATCAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTa < 8:62782/149‑1 (MQ=255) gCGTCTTGCGATAAAGTGTTGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTAt < 4:294920/149‑1 (MQ=255) cgtcgtGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATc > 7:12953/1‑149 (MQ=255) tcgtGCGATAAAGTGTGGACATGAGTCACCCCAAGGCCAGAATGAACCCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGGTATCgg < 6:228011/149‑1 (MQ=255) tGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGgctg > 7:38516/1‑149 (MQ=255) gATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCtt > 2:20356/1‑149 (MQ=255) aTAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTTTAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTtg > 4:105856/1‑149 (MQ=255) aGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGc > 1:220513/1‑149 (MQ=255) aGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCAAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGc > 8:249898/1‑149 (MQ=255) gtgGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGc > 3:92071/1‑149 (MQ=255) aTGAGTCAACCCAAGTCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGa < 6:222843/149‑1 (MQ=255) aTGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGa < 1:5502/149‑1 (MQ=255) tCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTc > 6:264358/1‑82 (MQ=255) tCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTc < 5:264358/82‑1 (MQ=255) tCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGcc > 1:125407/1‑149 (MQ=255) aaCCCAAGTCCAGAATGAAACCGGCAGCCCAATATTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTg < 5:45874/149‑1 (MQ=255) aCCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCAAGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTgg < 1:266694/149‑1 (MQ=255) cccAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTggg < 2:238007/149‑1 (MQ=255) ccAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGc < 8:12953/149‑1 (MQ=255) aGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCaa < 6:172984/149‑1 (MQ=255) gCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTgg < 1:88995/142‑1 (MQ=255) gCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTgg > 2:88995/1‑142 (MQ=255) cAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCTAGTGCGTCGCCTGTTTGTTATCTGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGccc > 6:116606/1‑149 (MQ=255) attaaaCCGGCAGCCTAATAGTATTTGCCCGTGACGTCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAgg < 8:201565/146‑1 (MQ=255) aaCCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCt < 2:125407/149‑1 (MQ=255) aaCCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGGTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCt < 4:231869/149‑1 (MQ=255) cTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCtgt < 7:249898/148‑1 (MQ=255) aTAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTgtggtg > 4:224213/1‑149 (MQ=255) tAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGc > 8:184754/1‑149 (MQ=255) gTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGCTGGAGCCTGAcc < 6:75497/149‑1 (MQ=255) tGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGCTGGAGCCTGAc > 4:171571/1‑147 (MQ=255) tGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGCTGGAGCCTGACca < 5:50211/149‑1 (MQ=255) cGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGCTGGAGCCTGACcagca < 5:184541/149‑1 (MQ=255) ggCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGCTGGAGCCTGACcagcag < 8:38516/149‑1 (MQ=255) cATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGCTGGAg < 7:234081/134‑1 (MQ=255) cATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGCTGGAg > 8:234081/1‑134 (MQ=255) gctgcATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCATCTGTGGTGCTGGAGCCTGACCAGCAGCAGTgg > 8:69909/1‑149 (MQ=255) | CATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAAGTCGAGCGTATAGATGAAGCGAGTGCGTCGCCTGTTGGTTATCGGCTGCTTGTGGCTGCCCTTGATTGCCTGGGCCAAGCCCGAGGCGCTGCCCTCTGTGGTGCTGGAGCCTGACCAGCAGCAGTGG > NC_002947/495955‑496242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |