Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	503,849	C→T	13.8%	intergenic (+52/‑99)	PP_0414 → / → rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	503,849	0	C	T	13.8%	144.5 / 11.5	58	intergenic (+52/‑99)	PP_0414/rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (22/28);  new base T (5/3);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

CGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTCTCTCTGCCGATT  >  NC_002947/503703‑503990                                                                                                                                                    |                                                                                                                                              cgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGcac                                                                                                                                             <  2:27033/149‑1 (MQ=255)      ttGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGtt                                                                                                                                        >  1:138934/1‑149 (MQ=255)        gCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGCACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGtt                                                                                                                                        <  1:104752/147‑1 (MQ=255)             ttGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTg                                                                                                                                  <  2:133880/148‑1 (MQ=255)               gCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCGCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGt                                                                                                                                <  2:271802/148‑1 (MQ=255)                cGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                                               <  7:293844/148‑1 (MQ=255)                  ttCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGac                                                                                                                            <  2:138934/149‑1 (MQ=255)                        gtgtTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAAt                                                                                                                      <  2:119962/149‑1 (MQ=255)                             gttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATa                                                                                                                 >  4:290225/1‑149 (MQ=255)                              ttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAc                                                                                                                <  5:212190/149‑1 (MQ=255)                                gctgctGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAcc                                                                                                               >  1:302577/1‑148 (MQ=255)                                  tgctgACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCaa                                                                                                             >  8:189405/1‑148 (MQ=255)                                   gctgACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAAt                                                                                                            >  8:216463/1‑148 (MQ=255)                                      gACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGc                                                                                                        >  1:13155/1‑149 (MQ=255)                                         ccTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCt                                                                                                      >  1:5828/1‑148 (MQ=255)                                                tGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGc                                                                                               <  7:189405/148‑1 (MQ=255)                                                  gTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCttt                                                                                            <  6:180776/149‑1 (MQ=255)                                                  gTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCttt                                                                                            <  2:195314/149‑1 (MQ=255)                                                   ttGCATGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTc                                                                                           >  2:119761/1‑149 (MQ=255)                                                      cGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTCCATCGCTTTCCCt                                                                                        >  4:83543/1‑149 (MQ=255)                                                        tGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGa                                                                                      <  7:216463/149‑1 (MQ=255)                                                          ggCGTAGCGATAAGCTGAGGACTCAACAGCCGGCTTGAAGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAg                                                                                     <  2:204330/148‑1 (MQ=255)                                                             gTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTAc                                                                                 <  8:305759/149‑1 (MQ=255)                                                                 cGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAAt                                                                              <  2:5828/148‑1 (MQ=255)                                                                   agaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcg                                                                           >  7:266713/1‑149 (MQ=255)                                                                    gaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcgc                                                                          <  2:302577/149‑1 (MQ=255)                                                                     aaGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcgcg                                                                         >  6:167823/1‑149 (MQ=255)                                                                        cTGAGGTCTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCTGGTAATGCCG‑GAAGAGGCCTGCACAGGTTGCGTTGGTCGACACCAATTGATACCAAt                                                                                                            <  7:9085/111‑1 (MQ=255)                                                                               cTCAACAGCCGGCTTGATTATGGCTTGCCTGTACGGGCCTCTTCGGGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGaa                                                               <  2:305701/149‑1 (MQ=255)                                                                               cTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGaa                                                               >  1:305701/1‑149 (MQ=255)                                                                                  aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGGGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGa                                                                                      <  2:64757/123‑1 (MQ=255)                                                                                  aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGa                                                                                      >  1:64757/1‑123 (MQ=255)                                                                                       ccGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCaa                                                       <  8:266713/149‑1 (MQ=255)                                                                                         ggCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCaa                                                       >  5:263078/1‑147 (MQ=255)                                                                                          gCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAg                                                    >  5:252456/1‑149 (MQ=255)                                                                                          gCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAg                                                    <  6:252456/149‑1 (MQ=255)                                                                                               atgatgGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTgc                                                <  5:167823/148‑1 (MQ=255)                                                                                                 gatgGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTgcgc                                              >  1:202908/1‑148 (MQ=255)                                                                                                      cTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTCCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCa                                        <  3:83543/149‑1 (MQ=255)                                                                                                          ccTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGccc                                    >  3:127959/1‑149 (MQ=255)                                                                                                           cTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGccc                                    >  4:53749/1‑148 (MQ=255)                                                                                                            tGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGc                                      <  2:35630/145‑1 (MQ=255)                                                                                                            tGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGc                                      >  1:35630/1‑145 (MQ=255)                                                                                                             gTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTAGCCAACAGGTGCGCCATGCAGCCCTAc                                 <  6:108270/149‑1 (MQ=255)                                                                                                              tACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACg                                >  5:78981/1‑149 (MQ=255)                                                                                                                 cGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTa                             <  6:78981/149‑1 (MQ=255)                                                                                                                  ggCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAt                            >  3:155600/1‑149 (MQ=38)                                                                                                                  ggCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAt                            >  1:161028/1‑149 (MQ=38)                                                                                                                   gCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAtt                           <  2:13155/149‑1 (MQ=255)                                                                                                                   gCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTTCGCCATGCAGCCCTAc                                 <  4:289204/143‑1 (MQ=38)                                                                                                                   gCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTAc                                 >  3:289204/1‑143 (MQ=38)                                                                                                                     ctctTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACAGTGAATTTGCCAACAGGTGCGCCATGAAGCCCTACGCTATTGc                         >  7:201774/1‑149 (MQ=255)                                                                                                                      tctTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATCCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGcc                        >  1:266460/1‑149 (MQ=255)                                                                                                                       tcttccgGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAtt                           >  7:192074/6‑146 (MQ=39)                                                                                                                       tcttccgGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAtt                           >  7:197127/6‑146 (MQ=39)                                                                                                                       tcttccgGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAtt                           <  8:192074/141‑1 (MQ=39)                                                                                                                       tcttccgGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAtt                           <  8:197127/141‑1 (MQ=39)                                                                                                                           gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGTCCCCt                    <  4:127959/148‑1 (MQ=255)                                                                                                                                              ggCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTCTCTCTGCCGAtt  <  3:290225/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              CGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTCTCTCTGCCGATT  >  NC_002947/503703‑503990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑