Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	504,929	C→A	7.7%	P104H (CCC→CAC)	PP_0416 →	phosphoglycolate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	504,929	0	C	A	7.7%	196.4 / 5.4	65	P104H (CCC→CAC)	PP_0416	phosphoglycolate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (25/35);  new base A (3/2);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

GTTGGCAATGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGATCATTGGTGGCGACACCT  >  NC_002947/504784‑505075                                                                                                                                                  |                                                                                                                                                    gTTGGCAATGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCgg                                                                                                                                                  >  6:146685/1‑149 (MQ=255)  ttGGCAATGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGgt                                                                                                                                                 <  2:65256/149‑1 (MQ=255)     gCAATGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGgtgt                                                                                                                                               <  2:211794/148‑1 (MQ=255)     gCAATGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGGGGTAGACCACGgtaag                                                                                                                                              >  7:240175/1‑146 (MQ=255)      cAATGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAATCCACGAACTTACCGGGGTAGACCACGgtaagt                                                                                                                                             <  1:39691/149‑5 (MQ=255)        aTGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTgcgc                                                                                                                                           <  1:290578/149‑1 (MQ=255)        aTGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTgcgc                                                                                                                                           <  2:326294/149‑1 (MQ=255)         tGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTgcgc                                                                                                                                           >  8:200518/1‑148 (MQ=255)           gcgcCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGa                                                                                                                                         <  5:6151/148‑1 (MQ=255)               ccAGGTGCTGGTGCGCCGCGCGTAGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGAAGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACAccc                                                                                                                                    >  6:96636/1‑149 (MQ=255)                 aGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTg                                                                                                                                  <  1:200615/149‑1 (MQ=255)                      cTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCgctg                                                                                                                             >  5:130861/1‑149 (MQ=255)                      cTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCgct                                                                                                                              <  3:184106/148‑1 (MQ=255)                         gTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCgctggct                                                                                                                          >  7:118819/1‑149 (MQ=255)                              ccgcGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTgcgc                                                                                                                      >  8:3899/1‑148 (MQ=255)                              ccgcGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTgcgc                                                                                                                      >  8:15550/1‑148 (MQ=255)                                         cGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTg                                                                                                          <  7:200518/149‑1 (MQ=255)                                           gggggCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGga                                                                                                        <  7:141475/149‑1 (MQ=255)                                                cATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATgg                                                                                                   >  3:295600/1‑149 (MQ=255)                                                 aTCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGgc                                                                                                  >  5:68194/1‑149 (MQ=255)                                                        aTGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATc                                                                                           <  7:15550/149‑1 (MQ=255)                                                           ccGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCAc                                                                                         >  3:284584/1‑148 (MQ=255)                                                                  ggACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCgctggc                                                                                                                           <  2:88780/107‑1 (MQ=255)                                                                  ggACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGc                                                                                 >  5:174865/1‑149 (MQ=255)                                                                  ggACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGc                                                                                 >  2:89631/1‑149 (MQ=255)                                                                   gACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGTTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGcc                                                                                >  5:12145/1‑149 (MQ=255)                                                                   gACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGc                                                                                 <  8:118819/148‑1 (MQ=255)                                                                      gATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑t                                                                              >  7:243791/1‑147 (MQ=255)                                                                            cTGGCAGAACAGGCGCTGGCGCTGTTCAGGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCtt                                                                      <  4:199436/149‑1 (MQ=255)                                                                             tGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGTTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCttt                                                                     <  6:12145/149‑1 (MQ=255)                                                                               gCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGACAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTttg                                                                    <  2:322946/148‑1 (MQ=255)                                                                               gCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGgcg                                                                                                 >  8:215798/1‑120 (MQ=255)                                                                               gCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGgcg                                                                                                 <  7:215798/120‑1 (MQ=255)                                                                                cAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTttgtt                                                                  <  5:200101/149‑1 (MQ=255)                                                                                 aGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTg                                                                                              >  4:28250/1‑121 (MQ=255)                                                                                 aGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTg                                                                                              <  3:28250/121‑1 (MQ=255)                                                                                 aGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTttgttg                                                                 <  7:3899/149‑1 (MQ=255)                                                                                    aCAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGcc                                                              >  1:148028/1‑149 (MQ=255)                                                                                    aCAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGcc                                                              <  2:148028/149‑1 (MQ=255)                                                                                     cAGGCGCTGGCGCTGTTTATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGc                                                                                                             <  6:97239/102‑1 (MQ=255)                                                                                     cAGGCGCTGGCGCTGTTTATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGc                                                                                                             >  5:97239/1‑102 (MQ=255)                                                                                      aGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCg                                                            >  1:284762/1‑149 (MQ=255)                                                                                          gctggcgctgTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGATGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCgctgc                                                        >  7:48210/1‑149 (MQ=255)                                                                                              gcgctgTTCATTGACTCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGAGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGctgctg                                                      <  2:155430/147‑1 (MQ=255)                                                                                               cgctgTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGAc                                                   <  6:130861/149‑1 (MQ=255)                                                                                                  tgTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAg                                                <  8:243791/149‑1 (MQ=255)                                                                                                   gTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGAc                                                   >  7:32843/1‑145 (MQ=255)                                                                                                    ttCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGa                                               <  2:284762/148‑1 (MQ=255)                                                                                                      cATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATg                                             >  6:193443/1‑148 (MQ=255)                                                                                                       aTGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGa                                            <  8:32843/148‑1 (MQ=255)                                                                                                        tGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGATATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAg                                          <  5:96636/149‑1 (MQ=255)                                                                                                             gCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTgg                                                     <  3:318883/133‑1 (MQ=255)                                                                                                             gCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTgg                                                     >  4:318883/1‑133 (MQ=255)                                                                                                                  tGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAAt                                 <  1:4841/148‑1 (MQ=255)                                                                                                                  tGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGGGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGATGCTGGACCAGATGAAGATCGGTAAt                                 <  1:10114/148‑1 (MQ=255)                                                                                                                     cGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATttct                             >  4:292638/1‑149 (MQ=255)                                                                                                                      gAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATttctt                            <  4:284584/149‑1 (MQ=255)                                                                                                                                 gcacaCCGGGGTAGACCACGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGATCa                 >  7:1101/5‑149 (MQ=255)                                                                                                                                 ccTTACGGTGGTCTACCCGGGTGTGCGCGAAACCCTGCGCTGGCTGGGAATGATGGGCGGGTTTATGGCGCTTATCACAAACAAGCCTTTCCCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGa                    <  1:89631/146‑1 (MQ=255)                                                                                                                                  cacaCCGGGGTAGACCACGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGATCAt                >  8:227050/4‑149 (MQ=255)                                                                                                                                  cTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGATCAt                <  3:292638/149‑1 (MQ=255)                                                                                                                                     aCCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGATCATtgg             >  8:1895/1‑149 (MQ=255)                                                                                                                                      ccGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGATCATtggt            <  5:146685/149‑1 (MQ=255)                                                                                                                                         gggtagaCCACGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGATCATTGGTGGc         <  7:227050/143‑1 (MQ=255)                                                                                                                                           gTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGATCATTGGTGGCGa       <  7:1895/149‑1 (MQ=255)                                                                                                                                            atgtccccGGTGTGTGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTTGAGATGGCGCTGATCACCAACAAGCG‑TGAGCGCTTTGTTGGGCCGCTGCTGGACCAGATGAAGTTCGGTAATTTCTTCCGCTGGATCATTGGTGGCGac      <  1:261649/145‑1 (MQ=255)                                                                                                                                                 cccGGAGTGAGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCCCTTAGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGATGAATCATTGGTGGCGacaaga  >  2:261649/1‑145 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GTTGGCAATGGCGCCCAGGTGCTGGTGCGCCGCGCGTTGGCGGGGGGCATCGACCATGCCGACGTGGACGATGCGCTGGCAGAACAGGCGCTGGCGCTGTTCATGGACGCTTATGCCGAAAGCCACGAACTTACCGTGGTCTACCCCGGTGTGCGCGACACCCTGCGCTGGCTGCGCAAGCAGGGCGTGGAGATGGCGCTGATCACCAACAAGCC‑TGAGCGCTTTGTTGGCCCGCTGCTGGACCAGATGAAGATCGGTAATTTCTTCCGCTGGATCATTGGTGGCGACACCT  >  NC_002947/504784‑505075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑