Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	536,422	T→G	5.5%	noncoding (36/190 nt)	PP_mr06 ←	P27 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	536,422	0	T	G	5.5%	217.5 / 3.7	73	noncoding (36/190 nt)	PP_mr06	P27 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (32/37);  new base G (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

CCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAGCG  >  NC_002947/536278‑536567                                                                                                                                                  |                                                                                                                                                   ccAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGc                                                                                                                                                >  7:221190/1‑149 (MQ=255)  tggTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGc                                                                                                                                                >  7:96694/3‑148 (MQ=255)  cAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTT‑CCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGc                                                                                                                                                >  4:213395/1‑147 (MQ=255)     tCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAg                                                                                                                                             >  4:191629/1‑148 (MQ=255)     tCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGCGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGa                                                                                                                                            <  3:95398/149‑1 (MQ=255)       gcgcCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCCAAAACGACATCCGGCCTCGGCCAGa                                                                                                                                            <  1:14866/148‑1 (MQ=255)        cgccgcCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGa                                                                                                                                            >  6:145062/1‑146 (MQ=255)        cgccgcCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGa                                                                                                                                            <  5:145062/146‑1 (MQ=255)              catcAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGt                                                                                                                                   <  7:178128/146‑1 (MQ=255)                   aaaaTTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTaaaaa                                                                                                                              <  8:96694/149‑1 (MQ=255)                   aaaaTTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAACCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTaaaaa                                                                                                                              >  2:145306/1‑149 (MQ=255)                      aTTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCa                                                                                                                           <  7:148267/149‑1 (MQ=255)                       ttGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGGGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAg                                                                                                                          <  5:89197/149‑1 (MQ=255)                       ttGCGGTCCCTGCGTGGCGGGGGCTATCCAAGAGCGTAGGCGTCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTTCTCCCGATTCGTTTCCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAg                                                                                                                          <  6:186166/149‑1 (MQ=255)                          gggTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                        <  6:64481/148‑1 (MQ=255)                          gggTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                        <  3:302870/148‑1 (MQ=255)                          gggTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                        <  2:41099/148‑1 (MQ=255)                            gTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGt                                                                                                                      >  4:150428/1‑148 (MQ=255)                               cctgcctgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAAc                                                                                                                  >  8:87091/1‑149 (MQ=255)                               cctgcatgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTaaa                                                                                                                   <  7:101721/148‑1 (MQ=255)                                   cctgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGt                                                                                                              <  1:86367/149‑1 (MQ=255)                                     tgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCTGGCCGAGGCCGGATgtcgttt                                                                                                                                    >  6:160974/1‑119 (MQ=255)                                     tgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCTGGCCGAGGCCGGATgtcgttt                                                                                                                                    <  5:160974/125‑7 (MQ=255)                                        cGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCTATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCaa                                                                                                         <  3:191629/149‑1 (MQ=255)                                          ggggCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                        <  3:199448/132‑1 (MQ=255)                                          ggggCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATACGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                        >  4:199448/1‑132 (MQ=255)                                             gCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTaaa                                                                                                    <  3:68559/149‑1 (MQ=255)                                             gCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTaa                                                                                                     >  5:86602/1‑148 (MQ=255)                                             gCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTT‑CCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAAc                                                                                                   <  3:213395/149‑1 (MQ=255)                                              cTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACAGTAAAAACCAGGAGTAAACCCGTGGCAATTAAAc                                                                                                   <  1:206537/149‑1 (MQ=255)                                               tATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACt                                                                                                  >  2:48543/1‑149 (MQ=255)                                                 tCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACt                                                                                                  <  3:304448/147‑1 (MQ=255)                                                 tCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACt                                                                                                  <  3:309441/147‑1 (MQ=255)                                                 tCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑Cg                                                                                               <  3:232156/149‑1 (MQ=255)                                                   cAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGaa                                                                                             <  5:57083/149‑1 (MQ=255)                                                   cAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGaa                                                                                             <  7:87091/149‑1 (MQ=255)                                                    aaGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCCCGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑Cg                                                                                               >  6:329569/1‑146 (MQ=255)                                                    aaGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAGCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGAAAACCCGTGGCAATTAAACT‑Cg                                                                                               >  5:73018/1‑146 (MQ=255)                                                       aCCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGAc                                                                                          >  5:18927/1‑148 (MQ=255)                                                           tAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACaag                                                                                       >  8:136169/1‑147 (MQ=255)                                                             ggcggcGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAgg                                                                                   <  6:86602/149‑1 (MQ=255)                                                                      aGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAACCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCAtcgtcg                                                                          <  1:145306/149‑1 (MQ=255)                                                                            aaaaCATCAGCCCAAGCAGATGGTGATCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAg                                                                    >  3:201826/1‑149 (MQ=255)                                                                                  tCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAACCCCGTGGCAATTAAACT‑CGATGCAAAGAAGCCAATCGAGGGTGAATTCAAct                                                              >  3:230524/1‑148 (MQ=255)                                                                                  tCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCTCTGAAGTCAAc                                                               >  2:195466/1‑148 (MQ=255)                                                                                   cAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACg                                                              <  1:48543/149‑1 (MQ=255)                                                                                        cACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTg                                                        >  5:314010/1‑149 (MQ=255)                                                                                        cACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CC‑AAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGc                                                       >  1:16256/1‑149 (MQ=255)                                                                                                      tCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTg                                          >  7:211476/1‑149 (MQ=255)                                                                                                       cccGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGt                                         >  6:303532/1‑149 (MQ=255)                                                                                                        ccGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTc                                        >  7:114437/1‑149 (MQ=255)                                                                                                        ccGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTc                                        <  7:136169/149‑1 (MQ=255)                                                                                                         cGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTcc                                       <  3:150428/149‑1 (MQ=255)                                                                                                           aTTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGc                                     >  8:283036/1‑149 (MQ=255)                                                                                                               gTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTc                                 >  1:309544/1‑149 (MQ=255)                                                                                                                     gAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCt                           <  5:111472/149‑1 (MQ=255)                                                                                                                     gAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCt                           <  3:63464/149‑1 (MQ=255)                                                                                                                      aaTCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCt                           >  7:109831/1‑148 (MQ=255)                                                                                                                           gACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACAAGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATAGTCGCTGAAGTCAACGAGGCTGCCAAAGTCTCTCTGTCCGATGTCGTGGCTTATgac                     >  8:246270/1‑147 (MQ=255)                                                                                                                                 cAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCgtgg                >  6:82718/1‑149 (MQ=255)                                                                                                                                  aaaaCGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCgtggt               <  2:309544/149‑1 (MQ=255)                                                                                                                                     aCGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGtgtg            <  5:329569/149‑1 (MQ=255)                                                                                                                                      cGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGGTGCCCGTGGtgtgg           <  3:73507/149‑2 (MQ=255)                                                                                                                                      cGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGa           <  8:211476/149‑1 (MQ=255)                                                                                                                                          aTCTGGCCGAGGCCGGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAgg                                                                                   <  2:137029/73‑1 (MQ=255)                                                                                                                                          aTCTGGCCGAGGCCGGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAgg                                                                                   >  1:137029/1‑73 (MQ=255)                                                                                                                                          aTCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGt       <  1:195466/149‑1 (MQ=255)                                                                                                                                            ccGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGt       >  4:270922/1‑147 (MQ=255)                                                                                                                                             cGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCgtg                 >  6:29076/1‑136 (MQ=255)                                                                                                                                             cGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCgtg                 <  5:29076/136‑1 (MQ=255)                                                                                                                                             cGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAg    >  7:219149/1‑149 (MQ=255)                                                                                                                                               gCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAgcg  <  3:270922/149‑1 (MQ=255)                                                                                                                                               gCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGGGAGTGTAAgcg  >  8:89488/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAGCG  >  NC_002947/536278‑536567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑