Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	536,428	A→G	5.5%	noncoding (30/190 nt)	PP_mr06 ←	P27 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	536,428	0	A	G	5.5%	218.1 / 2.1	72	noncoding (30/190 nt)	PP_mr06	P27 ncRNA
Reads supporting (aligned to +/- strand):  ref base A (30/38);  new base G (2/2);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

TCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAGCGCAATGA  >  NC_002947/536282‑536573                                                                                                                                                    |                                                                                                                                                   tCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAg                                                                                                                                                   >  4:191629/1‑148 (MQ=255) tCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGCGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGa                                                                                                                                                  <  3:95398/149‑1 (MQ=255)   gcgcCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCCAAAACGACATCCGGCCTCGGCCAGa                                                                                                                                                  <  1:14866/148‑1 (MQ=255)    cgccgcCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGa                                                                                                                                                  >  6:145062/1‑146 (MQ=255)    cgccgcCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGa                                                                                                                                                  <  5:145062/146‑1 (MQ=255)          catcAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGt                                                                                                                                         <  7:178128/146‑1 (MQ=255)               aaaaTTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTaaaaa                                                                                                                                    <  8:96694/149‑1 (MQ=255)               aaaaTTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAACCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTaaaaa                                                                                                                                    >  2:145306/1‑149 (MQ=255)                  aTTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCa                                                                                                                                 <  7:148267/149‑1 (MQ=255)                   ttGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGGGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAg                                                                                                                                <  5:89197/149‑1 (MQ=255)                   ttGCGGTCCCTGCGTGGCGGGGGCTATCCAAGAGCGTAGGCGTCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTTCTCCCGATTCGTTTCCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAg                                                                                                                                <  6:186166/149‑1 (MQ=255)                      gggTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                              <  2:41099/148‑1 (MQ=255)                      gggTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                              <  3:302870/148‑1 (MQ=255)                      gggTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                              <  6:64481/148‑1 (MQ=255)                        gTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGt                                                                                                                            >  4:150428/1‑148 (MQ=255)                           cctgcctgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAAc                                                                                                                        >  8:87091/1‑149 (MQ=255)                           cctgcatgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTaaa                                                                                                                         <  7:101721/148‑1 (MQ=255)                               cctgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGt                                                                                                                    <  1:86367/149‑1 (MQ=255)                                 tgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCTGGCCGAGGCCGGATgtcgttt                                                                                                                                          >  6:160974/1‑119 (MQ=255)                                 tgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCTGGCCGAGGCCGGATgtcgttt                                                                                                                                          <  5:160974/125‑7 (MQ=255)                                    cGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCTATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCaa                                                                                                               <  3:191629/149‑1 (MQ=255)                                      ggggCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                              <  3:199448/132‑1 (MQ=255)                                      ggggCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATACGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                                                              >  4:199448/1‑132 (MQ=255)                                         gCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTaaa                                                                                                          <  3:68559/149‑1 (MQ=255)                                         gCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTaa                                                                                                           >  5:86602/1‑148 (MQ=255)                                         gCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTT‑CCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAAc                                                                                                         <  3:213395/149‑1 (MQ=255)                                          cTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACAGTAAAAACCAGGAGTAAACCCGTGGCAATTAAAc                                                                                                         <  1:206537/149‑1 (MQ=255)                                           tATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACt                                                                                                        >  2:48543/1‑149 (MQ=255)                                             tCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACt                                                                                                        <  3:304448/147‑1 (MQ=255)                                             tCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACt                                                                                                        <  3:309441/147‑1 (MQ=255)                                             tCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑Cg                                                                                                     <  3:232156/149‑1 (MQ=255)                                               cAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGaa                                                                                                   <  7:87091/149‑1 (MQ=255)                                               cAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGaa                                                                                                   <  5:57083/149‑1 (MQ=255)                                                aaGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCCCGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑Cg                                                                                                     >  6:329569/1‑146 (MQ=255)                                                aaGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAGCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGAAAACCCGTGGCAATTAAACT‑Cg                                                                                                     >  5:73018/1‑146 (MQ=255)                                                   aCCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGAc                                                                                                >  5:18927/1‑148 (MQ=255)                                                       tAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACaag                                                                                             >  8:136169/1‑147 (MQ=255)                                                         ggcggcGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAgg                                                                                         <  6:86602/149‑1 (MQ=255)                                                                  aGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAACCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCAtcgtcg                                                                                <  1:145306/149‑1 (MQ=255)                                                                        aaaaCATCAGCCCAAGCAGATGGTGATCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAg                                                                          >  3:201826/1‑149 (MQ=255)                                                                              tCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAACCCCGTGGCAATTAAACT‑CGATGCAAAGAAGCCAATCGAGGGTGAATTCAAct                                                                    >  3:230524/1‑148 (MQ=255)                                                                              tCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCTCTGAAGTCAAc                                                                     >  2:195466/1‑148 (MQ=255)                                                                               cAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACg                                                                    <  1:48543/149‑1 (MQ=255)                                                                                    cACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTg                                                              >  5:314010/1‑149 (MQ=255)                                                                                    cACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CC‑AAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGc                                                             >  1:16256/1‑149 (MQ=255)                                                                                                  tCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTg                                                >  7:211476/1‑149 (MQ=255)                                                                                                   cccGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGt                                               >  6:303532/1‑149 (MQ=255)                                                                                                    ccGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTc                                              >  7:114437/1‑149 (MQ=255)                                                                                                    ccGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTc                                              <  7:136169/149‑1 (MQ=255)                                                                                                     cGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTcc                                             <  3:150428/149‑1 (MQ=255)                                                                                                       aTTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGc                                           >  8:283036/1‑149 (MQ=255)                                                                                                           gTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTc                                       >  1:309544/1‑149 (MQ=255)                                                                                                                 gAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCt                                 <  3:63464/149‑1 (MQ=255)                                                                                                                 gAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCt                                 <  5:111472/149‑1 (MQ=255)                                                                                                                  aaTCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCt                                 >  7:109831/1‑148 (MQ=255)                                                                                                                       gACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACAAGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATAGTCGCTGAAGTCAACGAGGCTGCCAAAGTCTCTCTGTCCGATGTCGTGGCTTATgac                           >  8:246270/1‑147 (MQ=255)                                                                                                                             cAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCgtgg                      >  6:82718/1‑149 (MQ=255)                                                                                                                              aaaaCGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCgtggt                     <  2:309544/149‑1 (MQ=255)                                                                                                                                 aCGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGtgtg                  <  5:329569/149‑1 (MQ=255)                                                                                                                                  cGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGGTGCCCGTGGtgtgg                 <  3:73507/149‑2 (MQ=255)                                                                                                                                  cGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGa                 <  8:211476/149‑1 (MQ=255)                                                                                                                                      aTCTGGCCGAGGCCGGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAgg                                                                                         >  1:137029/1‑73 (MQ=255)                                                                                                                                      aTCTGGCCGAGGCCGGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAgg                                                                                         <  2:137029/73‑1 (MQ=255)                                                                                                                                      aTCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGt             <  1:195466/149‑1 (MQ=255)                                                                                                                                        ccGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGt             >  4:270922/1‑147 (MQ=255)                                                                                                                                         cGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCgtg                       >  6:29076/1‑136 (MQ=255)                                                                                                                                         cGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCgtg                       <  5:29076/136‑1 (MQ=255)                                                                                                                                         cGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAg          >  7:219149/1‑149 (MQ=255)                                                                                                                                           gCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAgcg        <  3:270922/149‑1 (MQ=255)                                                                                                                                           gCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGGGAGTGTAAgcg        >  8:89488/1‑149 (MQ=255)                                                                                                                                                ggCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAGCGCaa     <  6:18927/147‑1 (MQ=255)                                                                                                                                                 gCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAGCACAATGa  >  2:201324/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACA‑CCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACT‑CGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCCGCTGTCGTGGCTGATGCCCGTGGTGTGACTGTAAGCGCAATGA  >  NC_002947/536282‑536573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑