Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 537,572 G→T 8.1% intergenic (+102/‑51) PP_mr07 → / → rpoB P26 ncRNA/DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947537,5720GT 8.1% 262.8 / 11.6 86intergenic (+102/‑51)PP_mr07/rpoBP26 ncRNA/DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (36/43);  new base T (4/3);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

CTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTGGA  >  NC_002947/537424‑537720
                                                                                                                                                    |                                                                                                                                                    
cTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGgctg                                                                                                                                                      >  5:271275/1‑149 (MQ=255)
          aGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCccgccg                                                                                                                                             <  3:161400/148‑1 (MQ=255)
          aGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                                                                                                                                            <  3:86282/149‑1 (MQ=255)
          aGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                                                                                                                                            <  2:39034/149‑1 (MQ=255)
           tcgattggCTGTTGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCTGATAACGAGCTGCAAGACACCCATGTGTGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                                                                                                                                            <  6:209137/142‑1 (MQ=255)
           gCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                                                                                                                                            <  5:26962/148‑1 (MQ=255)
             gAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                                                                                                                                            >  2:101741/1‑146 (MQ=255)
              aaaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGgag                                                                                                                                         >  2:284712/1‑148 (MQ=255)
               aaGGCTGTTGGCTGGTGGCTTATGCCTCGGGGCTTTTTCCGTTATTGGTGGGCGGTCAGGCCGGTCCCGATAACGAGCTGGTAGACAGCCATGTGGGTGGCGCAAAGCAAGGGGTTTGCACGATTTTCTGGGTGCTGCGGCCGGGAGaa                                                                                                                                       <  6:8696/149‑1 (MQ=255)
               aaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa                                                                                                                                       <  1:1433/149‑1 (MQ=255)
                aGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAg                                                                                                                                      <  4:107025/149‑1 (MQ=255)
                  gaaaaaggCCGGTGGCATAAGCCACCAGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGc                                                                                                                                     >  8:3946/7‑148 (MQ=255)
                  gCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGAGCAGcc                                                                                                                                    <  6:159940/149‑1 (MQ=255)
                    tGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGagcagc                                                                                                                                     >  1:321098/1‑146 (MQ=255)
                    tGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGagcagc                                                                                                                                     <  2:321098/146‑1 (MQ=255)
                      aTGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAAc                                                                                                                                <  5:61476/149‑1 (MQ=255)
                       tGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGAGCAGCCa                                                                                                                                   <  3:241661/145‑1 (MQ=255)
                       tGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGAGCAGCCa                                                                                                                                   >  4:241661/1‑145 (MQ=255)
                         gCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAAc                                                                                                                                >  7:288357/1‑146 (MQ=255)
                         gCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACaa                                                                                                                              <  6:271275/148‑1 (MQ=255)
                         gCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAg                                                                                                                             <  5:53340/149‑1 (MQ=255)
                          cTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGc                                                                                                                            <  8:194461/149‑1 (MQ=255)
                           tggtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAg                                                                                                                             >  7:265008/1‑147 (MQ=255)
                            ggtcgCTTATGCCACCGGCCTTTTTCCGATATTGGTGGCCAGTCAGGCCGGTCCCGATAACGAGCTGAATGACACCCATGTGGCTGGCGCAATCCAAGGGGATTGCACGATTATCTGGCTGCTCCCGCCGTGAGAAGCCAAACAAGCAg                                                                                                                          >  8:227912/1‑149 (MQ=255)
                             gtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAg                                                                                                                          <  8:265008/148‑1 (MQ=255)
                                gCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTg                                                                                                                       <  4:319287/148‑1 (MQ=255)
                                gCTTATGCCACCGGCCTTTATCCGTTATTGGTGGCCGCTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGa                                                                                                                      <  7:227912/149‑1 (MQ=255)
                                        cACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTg                                                                                                              <  1:284712/149‑1 (MQ=255)
                                        cACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTg                                                                                                              <  5:12434/149‑1 (MQ=255)
                                        cACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTg                                                                                                              >  4:16188/1‑149 (MQ=255)
                                        cACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTg                                                                                                              >  4:210896/1‑149 (MQ=255)
                                         aCCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTgg                                                                                                             <  5:149114/149‑1 (MQ=255)
                                         aCCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTgg                                                                                                             <  3:16188/149‑1 (MQ=255)
                                         aCCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGCCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTgg                                                                                                             >  6:107094/1‑149 (MQ=255)
                                          ccGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTggg                                                                                                            >  6:120635/1‑149 (MQ=255)
                                          ccGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTggg                                                                                                            >  1:326799/1‑149 (MQ=255)
                                             gCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaaa                                                                                                                                 >  2:263419/1‑125 (MQ=255)
                                             gCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaaa                                                                                                                                 <  1:259350/125‑1 (MQ=255)
                                             gCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaaa                                                                                                                                 >  2:259350/1‑125 (MQ=255)
                                             gCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaaa                                                                                                                                 <  1:263419/125‑1 (MQ=255)
                                               cTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACg                                                                                                       >  5:87831/1‑149 (MQ=255)
                                                     ccGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATg                                                                                                 >  1:79115/1‑149 (MQ=255)
                                                      cGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATg                                                                                                 <  8:288357/148‑1 (MQ=255)
                                                          aTTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTa                                                                                            >  7:268955/1‑149 (MQ=255)
                                                              gtggCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGa                                                                                                   <  5:227420/138‑1 (MQ=255)
                                                              gtggCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGa                                                                                                   >  6:227420/1‑138 (MQ=255)
                                                              gtggCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCa                                                                                        >  5:120570/1‑149 (MQ=255)
                                                                      tCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGc                                                                                               <  7:96192/134‑1 (MQ=255)
                                                                      tCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGc                                                                                               >  8:91692/1‑134 (MQ=255)
                                                                      tCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGc                                                                                               <  7:91692/134‑1 (MQ=255)
                                                                      tCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGc                                                                                               >  8:96192/1‑134 (MQ=255)
                                                                       cAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTgag                                                                               <  8:268955/149‑1 (MQ=255)
                                                                          gCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGaaa                                                                            <  2:211773/149‑1 (MQ=255)
                                                                          gCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGaaa                                                                            <  3:210896/149‑1 (MQ=255)
                                                                          gCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGaa                                                                             >  3:143949/1‑148 (MQ=255)
                                                                              gTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAAc                                                                        <  8:67211/149‑1 (MQ=255)
                                                                                        cGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGc                                                                >  7:170825/1‑147 (MQ=255)
                                                                                               cAAGACACCCATGTGGGTGGCGCAAACCCAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCCAACACGCAGGTGACCACGCGGGGGAACGCTGAGGGCTTACGCATCCCCTGAGAAAACACGTATCCGCAAGGACtg                                                        >  5:239465/1‑147 (MQ=255)
                                                                                               cAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACttt                                                       <  6:62409/149‑1 (MQ=255)
                                                                                                  gACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAACCAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAg                                                     >  2:182146/1‑148 (MQ=255)
                                                                                                     acCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCaa                                                              <  8:141235/136‑1 (MQ=255)
                                                                                                     acCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCaa                                                              >  7:141235/1‑136 (MQ=255)
                                                                                                     acCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAg                                                 >  6:2039/1‑149 (MQ=255)
                                                                                                     acCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAg                                                 <  7:3946/149‑1 (MQ=255)
                                                                                                       ccATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGtt                                               >  6:172484/1‑149 (MQ=255)
                                                                                                       ccATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGtt                                               >  7:301782/1‑149 (MQ=255)
                                                                                                          tgtgGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGcc                                            <  2:326799/149‑1 (MQ=255)
                                                                                                              ggtggCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGa                                                           >  7:257354/1‑130 (MQ=255)
                                                                                                              ggtggCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGa                                                           <  8:257354/130‑1 (MQ=255)
                                                                                                                  gcgcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAACCAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCa                                    <  1:182146/149‑1 (MQ=255)
                                                                                                                   cgcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCAt                                   <  8:170825/149‑1 (MQ=255)
                                                                                                                    gcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATc                                                                   >  2:323643/1‑116 (MQ=255)
                                                                                                                    gcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATc                                                                   <  1:323643/116‑1 (MQ=255)
                                                                                                                    gcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCAt                                   <  7:48834/148‑1 (MQ=255)
                                                                                                                    gcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCAt                                   >  8:48834/1‑148 (MQ=255)
                                                                                                                           aaGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGc                           <  4:143949/149‑1 (MQ=255)
                                                                                                                                    gCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCACGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTAcctc                   <  5:107094/148‑1 (MQ=255)
                                                                                                                                      aCGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTg                >  7:293463/1‑149 (MQ=255)
                                                                                                                                       cGATTTTCTGGCTGACCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAAGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTgg               <  1:276410/149‑1 (MQ=255)
                                                                                                                                        gcttgtttGGCTTCTCCCGGCGGGAGCAGCCAGACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGc              >  2:177544/8‑149 (MQ=255)
                                                                                                                                        gcttgtttGGCTTCTCCCGGCGGGAGCAGCCAGACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGc              >  2:172426/8‑149 (MQ=255)
                                                                                                                                        gATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTg                <  8:259251/147‑1 (MQ=255)
                                                                                                                                        gATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTg                >  7:259251/1‑147 (MQ=255)
                                                                                                                                         aTTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGc              >  8:279281/1‑148 (MQ=255)
                                                                                                                                          ttttCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGTCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCa            <  5:120635/149‑1 (MQ=255)
                                                                                                                                          ttttCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCa            >  4:62698/1‑149 (MQ=255)
                                                                                                                                           tttCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCAGGACGTCATGGATGTGCCTTACCTCCTGGCCAt           >  4:3241/1‑149 (MQ=255)
                                                                                                                                                 gcttctCCCGGCGGGAGCAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCt     >  8:61028/1‑149 (MQ=255)
                                                                                                                                                 gctgcgCCCGCCGGGAGAAGCCAAACCAGCAGGTGACCAAGCTGGGGACCGCTGAGGGCGTACTCATACACTGAGAACAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGGCATGGATGTGCCTTACCTCCTGGCCATCCAGCt     <  6:239465/149‑1 (MQ=255)
                                                                                                                                                   tgctCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTg    >  6:28577/1‑148 (MQ=255)
                                                                                                                                                    gctCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTGGa  >  1:205483/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTGGA  >  NC_002947/537424‑537720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑