Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 587,076 C→G 7.9% P43R (CCG→CGG)  PP_0497 → Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947587,0760CG 7.9% 144.4 / 2.6 50P43R (CCG→CGG) PP_0497Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base G (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

GCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATCAGCGAGTTG  >  NC_002947/586929‑587221
                                                                                                                                                    |                                                                                                                                                 
gcgTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTcc                                                                                                                                                   >  1:285953/1‑148 (MQ=255)
     aCCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACGTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGc                                                                                                                                             >  1:72360/1‑149 (MQ=255)
       cTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCtt                                                                                                                                           <  2:189354/149‑1 (MQ=255)
        tGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCttt                                                                                                                                          >  5:222831/1‑149 (MQ=255)
         ggATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCttt                                                                                                                                          >  7:123285/1‑148 (MQ=255)
          gATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCGGACGGCtttgc                                                                                                                                        <  1:290530/149‑3 (MQ=255)
          gATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCttt                                                                                                                                          <  5:298150/147‑1 (MQ=255)
          gATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCa                                                                                                                                        <  4:160174/149‑1 (MQ=255)
            ttCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAAc                                                                                                                                      >  6:128051/1‑149 (MQ=255)
            ttCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAAc                                                                                                                                      >  6:141004/1‑149 (MQ=255)
              ccGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAAcc                                                                                                                                     <  2:72360/148‑1 (MQ=255)
                  atatGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCAGACGGCTTTCAACCTGAAg                                                                                                                                >  1:77589/1‑149 (MQ=255)
                   tatGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGc                                                                                                                               <  2:285953/149‑1 (MQ=255)
                           aTCACTTCGCTACAACCCTCAAACTTGCCTGCATGCACTACCGCTCTATTTCCGAAGTTTCCCGCCAGCTGTAGATCAACCGCGCGCAGTTCAACAAGTACCTGAGG‑GGTCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGc                                                                                                                       <  5:171629/149‑1 (MQ=255)
                                ttCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCGGACGGCtttgc                                                                                                                                        >  5:314032/1‑125 (MQ=255)
                                ttCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCGGACGGCtttgc                                                                                                                                        <  6:314032/127‑3 (MQ=255)
                                ttCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGa                                                                                                                     >  5:307433/1‑146 (MQ=255)
                                ttCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGa                                                                                                                     <  6:307433/146‑1 (MQ=255)
                                    cTACAAACCTCAAACTTGCCTGCAGTCACTNCCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACttcttc                                                                                                              >  1:27916/1‑149 (MQ=255)
                                    cTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACttcttc                                                                                                              >  1:32897/1‑149 (MQ=255)
                                    cTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACttcttc                                                                                                              <  3:47615/149‑1 (MQ=255)
                                       cAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCgg                                                                                                            <  7:64941/148‑1 (MQ=255)
                                         aaCCTGAAACTTGCCTTCAGTCAGTACCGCTCTATTTCGGTAGTTTGGCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTTCCTGTGCGGGGAAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGgtg                                                                                                          <  1:240275/149‑1 (MQ=255)
                                          aCCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTGCCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTc                                                                                                        >  1:297932/1‑149 (MQ=255)
                                             tCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCg                                                                                                             >  6:222084/1‑141 (MQ=255)
                                             tCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCg                                                                                                             <  5:222084/141‑1 (MQ=255)
                                               aaaCTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAg                                                                                                     <  8:123285/147‑1 (MQ=255)
                                                aaCTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGAt                                                                                                  <  5:128051/149‑1 (MQ=255)
                                                 aCTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCATCAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGAtt                                                                                                 <  4:106618/149‑1 (MQ=255)
                                                   ttGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTAc                                                                                               >  3:130214/1‑149 (MQ=255)
                                                   ttGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGAAGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTAc                                                                                               >  7:59154/1‑149 (MQ=255)
                                                                           tttCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTGCCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCa                                                                        <  2:297932/148‑1 (MQ=255)
                                                                               cGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCg                                                                   >  5:198119/1‑149 (MQ=255)
                                                                                           ccAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCg                                                       <  6:198119/149‑1 (MQ=255)
                                                                                                  tCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGcgcg                                                <  2:32897/149‑1 (MQ=255)
                                                                                                  tCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGcgcg                                                <  2:27916/149‑1 (MQ=255)
                                                                                                            gcgcgcAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGccc                                      >  3:227158/1‑149 (MQ=255)
                                                                                                              gcgcAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAAc                                                                                      >  4:33091/1‑99 (MQ=255)
                                                                                                              gcgcAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAAc                                                                                      <  3:33091/99‑1 (MQ=255)
                                                                                                               ctcAGTTCAACAAGTACCTGATC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAg                                   <  2:77589/147‑1 (MQ=255)
                                                                                                                    ttCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATAGCCCCAGGcc                                >  8:68906/1‑147 (MQ=255)
                                                                                                                    ccgatctaGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGCAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCa                              <  5:223/142‑1 (MQ=255)
                                                                                                                     tCAACAAGTAGCTGATC‑TGGCAAAGCCGTCCGTCTGATTTCAACCTGAAGCGTATCGGCGAATTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCGGCCTAGAAGTTCGCCCGTCTGATCTGTGCGCGGGTATCGCCCCAGGCCCa                              <  8:59154/148‑1 (MQ=255)
                                                                                                                         caaGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAAc                         >  5:169291/1‑149 (MQ=255)
                                                                                                                            gTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCa                              >  6:223/1‑141 (MQ=255)
                                                                                                                               ccTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGt                    >  6:53944/1‑148 (MQ=255)
                                                                                                                               ccTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGt                    >  4:121435/1‑148 (MQ=255)
                                                                                                                                  gAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGAcc                >  8:1162/1‑149 (MQ=255)
                                                                                                                                       gttgaaaGCCGTCGGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGAt            >  2:184395/5‑149 (MQ=255)
                                                                                                                                               ccGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATCAGCGAgt    <  6:222831/149‑1 (MQ=255)
                                                                                                                                                 gTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATCAGCGAgttg  >  5:182596/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGC‑GGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATCAGCGAGTTG  >  NC_002947/586929‑587221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑