Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	672,892	T→A	7.5%	I210F (ATC→TTC)	PP_0574 ←	LuxR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	672,892	0	T	A	7.5%	180.9 / 2.2	62	I210F (ATC→TTC)	PP_0574	LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base A (3/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGGGTCGTGAACCGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGCTTTTTC  >  NC_002947/672745‑673036                                                                                                                                                     |                                                                                                                                                 aaTGGGTCGTGAACCGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATc                                                                                                                                                 >  1:188838/1‑149 (MQ=255) aaTGGGTCGTGAACCGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATc                                                                                                                                                 <  6:277967/149‑1 (MQ=255)  aTGGGTCGTGAACCGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCAGAGACACATAGAGCTTC‑ACGGTGTGTTCGGTAATGCCCAGTTCGCAGGCGATCt                                                                                                                                                >  7:202648/1‑149 (MQ=255)     ggTCGTGAACCGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTgg                                                                                                                                              <  2:378/148‑1 (MQ=255)         gTGAACCGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAAtt                                                                                                                                         <  7:126636/149‑1 (MQ=255)             aCCGCTGGTGCACGGGTGACGAGCTGTGGGTCAGCGCCAGATCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑AAGTTGTTTTCGGTAATGACCATTTCGCAGGCGATCTGGTAATTGGTc                                                                                                                                     >  7:138695/1‑149 (MQ=255)             aCCGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTc                                                                                                                                     <  6:41559/149‑1 (MQ=255)              ccGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTct                                                                                                                                    <  7:95032/149‑1 (MQ=255)               cGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTg                                                                                                                                        >  6:41593/1‑144 (MQ=255)               cGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTg                                                                                                                                        <  5:41593/144‑1 (MQ=255)               cGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTg                                                                                                                                        <  3:171011/144‑1 (MQ=255)               cGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGAAGGCGATCTGGTAATTg                                                                                                                                        >  4:171011/1‑144 (MQ=255)                  tGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTcttgcc                                                                                                                                <  8:67745/149‑1 (MQ=255)                                cGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGgc                                                                                                                  <  5:78556/149‑1 (MQ=255)                                          gTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTc                                                                                                        >  3:264112/1‑149 (MQ=255)                                           tCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTc                                                                                                        >  5:215394/1‑148 (MQ=255)                                             aGCTCCATATCCAGCTGGGTGCGGTTTTGCATATGCGTCAAACGCAATACATGATACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCTATCTTGTAATTGGTATTTCCATTGCCTACCAGTCGCGCCACTTCCa                                                                                                      <  8:138695/148‑1 (MQ=255)                                             aGCACCAGATACAGCTGGTTGACTTAGTCCATATGCGTCAAACGCAATACCTGAGACACATACAGCTTC‑ACATTGATATCGGTAATGCCAACTACACAGTAGAACTTTTAATTGTTCTTGCCATTGAAAACCACGAGCACCACTTc                                                                                                        >  2:196662/1‑146 (MQ=255)                                                 ccAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTCGACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTg                                                                                                  >  1:96819/1‑149 (MQ=255)                                                   agagCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGGCCAGTTCGGAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCg                                                                                               >  4:20792/1‑149 (MQ=255)                                                       ccAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTg                                                                                             >  8:231773/1‑147 (MQ=255)                                                         aGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGgaga                                                                                         >  8:23522/1‑149 (MQ=255)                                                         aGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGgaga                                                                                         <  7:231773/149‑1 (MQ=255)                                                           cTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACa                                                                                       <  7:38616/149‑1 (MQ=255)                                                                tGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGgag                                                                                          >  6:24929/1‑141 (MQ=255)                                                                tGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGgag                                                                                          <  5:24929/141‑1 (MQ=255)                                                                tGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTtc                                                                                  <  4:165067/149‑1 (MQ=255)                                                                  cGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTtctc                                                                                <  2:188838/149‑1 (MQ=255)                                                                   ggTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTtctc                                                                                >  2:16670/1‑148 (MQ=255)                                                                   ggTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTtctc                                                                                >  3:248831/1‑148 (MQ=255)                                                                             atGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTAGCAGGCGATCTGGTAATTGGTCTTGCCATTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGctgctg                                                                     >  5:82762/1‑149 (MQ=255)                                                                               gCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTgg                                                                    >  5:14201/1‑148 (MQ=255)                                                                                        gCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCtcgcat                                                          >  7:142098/1‑147 (MQ=255)                                                                                          aaTACCTGAGACCCCTAGAGCTAC‑ACGGTGTCTTCGGTACTGCCCAGTTCGCAGGCCATCTTGTACTTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCtcgctttc                                                        <  8:298397/149‑1 (MQ=255)                                                                                          aaTACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCtcgctttc                                                        <  6:215394/149‑1 (MQ=255)                                                                                                  agaCACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCgctttcgctttc                                                  <  5:303773/147‑1 (MQ=255)                                                                                                  agaCACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTttctt                                                <  6:14201/149‑1 (MQ=255)                                                                                                      acaTAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTg                                             >  5:239337/1‑148 (MQ=255)                                                                                                             gCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTg                                     <  2:96819/149‑1 (MQ=255)                                                                                                              cTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTgg                                    >  5:257619/1‑149 (MQ=255)                                                                                                              cTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTgg                                    >  8:154656/1‑149 (MQ=255)                                                                                                                   aCGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACg                                >  8:76904/1‑149 (MQ=255)                                                                                                                         ttttCGGTAATGCCGAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCggt                           <  6:82762/148‑1 (MQ=255)                                                                                                                           ttCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCggtggg                        >  5:6769/1‑149 (MQ=255)                                                                                                                            tCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCggtgggt                       >  4:139517/1‑149 (MQ=255)                                                                                                                                  aTGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTg                 <  7:76904/149‑1 (MQ=255)                                                                                                                                      ccAGATCGCCTGCGAACTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCa             >  7:217101/1‑149 (MQ=255)                                                                                                                                      ccAGATCGCCTGCGAACTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCa             >  7:221281/1‑149 (MQ=255)                                                                                                                                       cAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCggtgggt                       >  6:112353/1‑138 (MQ=255)                                                                                                                                       cAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCggtgggt                       <  5:112353/138‑1 (MQ=255)                                                                                                                                       cAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATAACTGTCACGCCGGTGGGTGCCTTTGCCAc            >  1:23334/1‑149 (MQ=255)                                                                                                                                         gTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACgg          >  5:245072/1‑149 (MQ=255)                                                                                                                                         gTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACgg          >  3:12033/1‑149 (MQ=255)                                                                                                                                         gTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACgg          >  6:306386/1‑149 (MQ=255)                                                                                                                                            cGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGc        <  3:20792/148‑1 (MQ=255)                                                                                                                                             gcctgCGAACTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGCtt      <  8:217101/145‑1 (MQ=255)                                                                                                                                             gcctgCGAACTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGCtt      <  8:221281/145‑1 (MQ=255)                                                                                                                                               aGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGCtttt    <  4:264112/149‑1 (MQ=255)                                                                                                                                                 gCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGCTTTTTc  <  5:281567/149‑1 (MQ=255)                                                                                                                                                 gCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGCTTTTTc  <  6:239337/149‑1 (MQ=255)                                                                                                                                                 gCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTCGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGCTTTTTc  <  4:144650/149‑1 (MQ=255)                                                                                                                                                  cGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGCTTTTTc  >  3:275696/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                 AATGGGTCGTGAACCGCTGGTGCACGGGTGACGAGCTGGGGGTCAGCGCCAGAGCCAGCTGGGTGCGGTTGTGCATATGCGTCAAACGCAATACCTGAGACACATAGAGCTTC‑ACGGTGTTTTCGGTAATGCCCAGTTCGCAGGCGATCTGGTAATTGGTCTTGCCCTTGCCTACCAGGCGCGCCACTTCCAGCTGGCGTGGAGACAGTTTCTCGAACGCTGCTGGCAGCTCGCTTTCGCTTTCTTCTGCATCACTGGCACGCCGGTGGGTGCCTTGGCCACGGGCTTTTTC  >  NC_002947/672745‑673036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑