Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	710,379	T→C	8.0%	Y59Y (TAT→TAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	710,379	0	T	C	8.0%	135.5 / 2.6	50	Y59Y (TAT→TAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (21/25);  new base C (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

TTCCGGACACCGCCTTCCCCATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGCCGATCGAGCACAAGGTCGAGGT  >  NC_002947/710231‑710525                                                                                                                                                      |                                                                                                                                                    ttCCGGACACCGCCTTCCCCATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTAt                                                                                                                                                     >  5:271809/1‑149 (MQ=255) ttCCGGACACCGCCTTCCCCATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTAt                                                                                                                                                     >  2:294737/1‑149 (MQ=255)    cGGACACCGCCTTCCCCATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGc                                                                                                                                                   <  3:91795/148‑1 (MQ=255)     ggACACCGCCTTCCCCATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGcc                                                                                                                                                  >  1:178081/1‑148 (MQ=255)       acacCGCCTTCCCCATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAAc                                                                                                                                               <  8:127360/149‑1 (MQ=255)           cGCCTTCCCCATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAAc                                                                                                                                               >  5:269166/1‑145 (MQ=255)           cGCCTTCCCCATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAAc                                                                                                                                               <  6:269166/145‑1 (MQ=255)                   ccATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCACCGGCAAGATTca                                                                                                                                    >  2:317471/1‑148 (MQ=255)                   ccATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGAGTca                                                                                                                                    >  5:259029/1‑148 (MQ=255)                     aTGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACAt                                                                                                                                 >  8:143039/1‑149 (MQ=255)                     aTGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGAGCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGAGCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACAt                                                                                                                                 <  7:220393/149‑1 (MQ=255)                                 ctgcctCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCt                                                                                                                     <  2:178081/149‑1 (MQ=255)                                    cctCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAATTTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGaa                                                                                                                  >  8:113475/1‑149 (MQ=255)                                      tCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGCAc                                                                                                                 >  6:180679/1‑148 (MQ=255)                                      tCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGAATTGGCCTG‑TACCAGAAGCTGCGCGAAATAGGCAAGGATCGTCCCAACTTCGTCCTGCACGACGGCCCGCCCTATGCAAACGGCAACATTCACATCGGTCATGCGCTGaaa                                                                                                                 >  5:157358/1‑147 (MQ=255)                                              aaCCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTc                                                                                                        <  6:271809/149‑1 (MQ=255)                                              aaCCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTc                                                                                                        <  2:76555/149‑1 (MQ=255)                                                 cGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATAGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAg                                                                                                     <  3:163964/149‑1 (MQ=255)                                                   cAGATCCTGCAGCGCTGGGACAGCATTGGCCTGTTACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCAAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTAATGCGCTGaa                                                                                                                  >  1:204372/1‑135 (MQ=255)                                                   cAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGaa                                                                                                                  <  2:204372/134‑1 (MQ=255)                                                                  tGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTc                                                                                    <  7:143039/149‑1 (MQ=255)                                                                      aCAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGTTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAg                                                                                <  1:110160/149‑1 (MQ=255)                                                                          cATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGAccc                                                                            >  2:29833/1‑149 (MQ=255)                                                                          cATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTACGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAg                                                                                                     >  2:155581/1‑124 (MQ=255)                                                                          cATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTACGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAg                                                                                                     <  1:155581/124‑1 (MQ=255)                                                                          cATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTACGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAg                                                                                                     >  8:111201/1‑124 (MQ=255)                                                                          cATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTACGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAg                                                                                                     <  7:111201/124‑1 (MQ=255)                                                                                  tG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGc                                                                   >  7:198234/1‑149 (MQ=255)                                                                                        cAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGa                                                              >  1:303714/1‑149 (MQ=255)                                                                                        cAGAAGCTGAGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGa                                                              >  2:209194/1‑149 (MQ=255)                                                                                                    gAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGt                                                  <  8:198234/149‑1 (MQ=255)                                                                                                      aaTTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGc                                                <  7:245104/149‑1 (MQ=255)                                                                                                           gCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGACAGGt                                           >  5:249365/1‑149 (MQ=255)                                                                                                            cAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGtt                                          >  4:69925/1‑149 (MQ=255)                                                                                                             aaGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTg                                         <  1:147501/149‑1 (MQ=255)                                                                                                              aGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGGAGCGGCTTCTATGCGCCGTACGTGCCAGGTTg                                         <  1:29833/149‑1 (MQ=255)                                                                                                                    gTCCCAATTTCGGCCTGCACGACGGCCAGCCCTATGCCAACTGAAAGATTCACATCGGGCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGc                                  <  6:249365/149‑1 (MQ=255)                                                                                                                       ccAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCAc                               >  7:212154/1‑149 (MQ=255)                                                                                                                         aaGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACgg                             >  1:161037/1‑149 (MQ=255)                                                                                                                          aGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGc                            <  3:307412/149‑1 (MQ=255)                                                                                                                               gTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACATGCCAGGTTGGGACTGCCACGGCCTGc                        <  2:303714/148‑1 (MQ=255)                                                                                                                                tCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGcc                       >  5:181029/1‑148 (MQ=255)                                                                                                                                tCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGCCg                      <  1:209194/149‑1 (MQ=255)                                                                                                                                  cTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGCCGAt                    <  1:86337/149‑1 (MQ=255)                                                                                                                                      acgacgGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATACGCCGTACGTGCCAGGTTGGGACTGCCACGGc                            >  2:19061/1‑137 (MQ=255)                                                                                                                                      acgacgGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATACGCCGTACGTGCCAGGTTGGGACTGCCACGGc                            <  1:19061/137‑1 (MQ=255)                                                                                                                                        gacgGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGCCCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGCCTGCCACGGCCTGCCGATCGAGca              >  5:181720/1‑149 (MQ=255)                                                                                                                                        gacgGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGCCGATCGAGca              <  6:181029/149‑1 (MQ=255)                                                                                                                                            gcccgcccTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGCCGATCGAGCACAAg          <  1:317471/149‑1 (MQ=255)                                                                                                                                                 cccTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGAT‑‑GCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGCCGATCGAGCACAAGGTCGAgg   <  1:294737/149‑1 (MQ=255)                                                                                                                                                   cTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGCCGATCGAGCACAAGGTCGAgg   <  2:161037/149‑1 (MQ=255)                                                                                                                                                     aTGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGCCGATCGAGCACAAGGTCGAGGt  >  6:15131/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                    TTCCGGACACCGCCTTCCCCATGAAGGCCGGCCTGCCTCAGCGCGAACCGCAGATCCTGCAGCGCTGGGACAGCATTGGCCTG‑TACCAGAAGCTGCGCGAAATTGGCAAGGATCGTCCCAAGTTCGTCCTGCACGACGGCCCGCCCTATGCCAACGGCAAGATTCACATCGGTCATGCGCTGAACAAGATCCTCAAGGACATGATCGTCCGTTCCAAGACCCTGG‑CCGGCTTCGATGCGCCGTACGTGCCAGGTTGGGACTGCCACGGCCTGCCGATCGAGCACAAGGTCGAGGT  >  NC_002947/710231‑710525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑